induces apoptosis and inhibits multi-drug resistance transporters in human epithelial cancer cells. J. Ethnopharmacol. 158, 33-42 (2014). HPTLC fingerprint of the whole plant of Vernonia cinerea L. on silica gel with toluene - ethyl acetate - formic acid 6:3:1, detection at UV 366 nm. The dichloromethane fraction showed the presence of 9 zones, including the standard lupeol at hRF 60.

Food Chem. 210, 613-622 (2016). HPTLC fingerprinting of triterpenoids in Siam and Sumatra benzoin balsams on silica gel with n-hexane – methanol – acetic acid 8:2:1. Detection by dipping into anisaldehyde sulfuric acid reagent for 1 s (10 mL of sulfuric acid were carefully added to an ice-cold solution of 170 mL methanol and 20 mL acetic acid, followed by the addition of 1 mL of anisaldehyde (p-methoxybenzaldehyde)), followed by heating at 105 °C for 5 min. Qualitative identification under UV 366 nm. Two specific compounds at approximately hRF 5 (violet band) and 50 (beige band) were detected in the Sumatra sample. Siam benzoin is characterized by two specific compounds at approximately hRF 5 and 10 (brown bands) and two others at approximately hRF 20 and 60 (orange bands).

Planta Medica 82(18), 1546-1552 (2016). The fractionation of the butanol-soluble part of the methanol extract of Lysimachia ciliata was monitored through TLC on silica gel with chloroform – methanol – water 23:12:2, detection by spraying with sulfuric acid. The saponin-containing fractions were mixed and submitted to preparative TLC on silica gel (same mobile phase). Saponins, eluted with methanol from the water-sprayed layer, were analyzed by UPLC-ESI-MS/MS; two were identified as desglucoanagalloside B and anagallosaponin IV (29.1 % and 8.2 % of the saponin fraction, respectively).

J. Ethnopharmacol. 231, 494-502 (2019). HPTLC fingerprint of Albizia lebbeck on silica gel with toluene – ethyl acetate – formic acid 5:5:1. Qualitative identification under UV light at 276 nm. The hRF value for standard catechin was 48. Saponin rich fraction was also analyzed by HPTLC on silica gel with petroleum ether – ethyl acetate 1:1. Detection by spraying with anisaldehyde – sulfuric acid reagent, followed by heating at 110–120 °C for 10–12 min.

Anal. Biochem. 128, 323-330 (1983). TLC of glycosides on silica with chloroform - methanol - acetic acid - water 60:20:0.5:5. Detection by UV. Fluorometry.

J. Chromatogr. 325, 317-322 (1985). TLC of adenine, adenosine, inosine, hypoxanthine, 2-AMP, 5-AMP, ADP, ATP, cAMP and dibutyryl cAMP on silica with eleven developing systems. Determination by radioscanning or liquid scintillation counting.

Densitometry by absorbance at 254 nm.

J. Chromatogr. 405, 125-134 (1987). TLC on silica with new solvent system consisting of acetonitrile, isopropanol and aqueous 50 mM potassium chloride or 2.5M NH3. Optimization of the solvent ratios and salt composition. Two-dimensional TLC with the two systems.