J. Chromatogr. Sci. 56, 518-523 (2018). Presentation of a validated method for the quantitation of dihydrokaempferol-4′-O-glucopyranoside. This flavanonol glucoside is a marker compound in the flower of Alcea rosea L. with significant antioxidant and anticancer activity against the HepG-2 cell line. HPTLC on silica gel with ethyl acetate – methanol – water – acetic acid 600:100:80:3 over 70 mm with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 295 nm. The amount of dihydrokaempferol-4′-O-glucopyranoside in the flowers of A. rosea was 0.733 g/100 g and 0.928 g/100 g after maceration and sonication for 15 min, respectively. Linearity was in the concentration range of 0.9-3.6 µg/zone. The %RSD of the intra-day and inter-day precision was 0.2–1.5 % and 0.5–1.7 %, respectively. The LOD and LOQ were 312 ng/zone and 947 ng/zone, respectively.

(Recent trends in pharmaceutical steroid analysis). Two-dimensional TLC of digoxine on cellulose, impregnated with acetone - formamide 10:2, with 1) xylene - MEK 1:1, 2) chloroform. Detection by spraying with chloramine T and heating at 105°C for 30 minutes. Fluorometry.

( Chinese) - (TLC-densitometry of isoflavones in pueraria lobata.) TLC of daidzein, daidzin, puerarin and daidzein 4,7-diglucoside in the root of P. lobata and in tablets on silica with toluene - methanol -10 % formic acid 7:3:00.2, or ethyl acetate - methanol -50 % formic acid 8:2:0.2. Quantification by densitometry. Relative standard deviations: 1.51 % for puerarin, 1.61 % for daidzein.

J. High Resol. Chromatogr. 10, 362-365 (1987). Application of the "PRISMA" optimization model for TLC separation of ginsenosides. Separation of 8 ginsenosides on silica (TLC, HPTLC and OPLC) with MEK Methanol - water in different ratios 70:22:8. Densitometry by absorbance at 550 nm.

Optimization of TLC/HPTLC of ginsenosides. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 401-413. TLC/HPTLC on silica with chloroform - ethyl acetate - methanol - water 15:40:22:10. Visualization by dipping in 5% sulfuric acid - ethanol. Detection by fluorodensitometry. Discussion of the optimization of chromatographic conditions.

J. of Natural Products 51, 973-976 (1988). TLC on silica with ethyl acetate - methanol 9:1, chloroform - ethyl amine 9:1 and chloroform - methanol 9:1. Detection under UV and by spraying with ceric ammonium sulfate reagent.

Helv. Chim. Acta 72, 668-674 (1989). TLC of saponins with molluscicidal activity on silica with ethyl acetate - methanol - water 17:2:1 and HPTLC on RP-8 with methanol - water 7:3. Detection with Godin reagent. TLC of saponin hydrolysate on silica with ethyl acetate - methanol - water - acetic acid 65:15:15:20 and on cellulose with ethyl acetate - pyridine - acetic acid - water 5:5:1:3. Detection of sugar by spraying with p-anisidine phthalate and of glucosamines with ninhydrin. Also HPLC and medium pressure liquid chromatography.

J. Chinese Herb Med. (Zhongcaoyao) 20, 447-448 (1989). TLC of saponins on silica with butanol - ethanol - NH3 25% 7:4:5. Detection under UV light and by spraying with vanillin - sulfuric acid reagent and heating at 110 - 140°C.