Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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J. Agric. Food Chem. 50, 426-430 (2002). TLC of purified soyasaponins on silica gel with chloroform - methanol - water 13:7:2; lower phase. Visualization by saturation with 10% sulfuric acid and heating for 15 min at 120°C. Direct densitometry on the plate against soyasaponin standards (soyasaponin I,II,III) after application without solvent development. Densitometry in reflectance mode. Improved and simple method.
J. Planar Chromatogr. 18, 167-170 (2005). TLC and HPTLC of saponins from 70 species of Acer on silica gel in a horizontal chamber with chloroform - methanol - formic acid - water 200:80:20:19. Detection by spraying with 10 % sulfuric acid in ethanol or anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 5 min, and evaluation in visible and UV light at 366 nm. Detection also by spraying with water or blood reagent.
CBS 109, 10-12 (2012). HPTLC of steviol glycosides (stevioside, rebaudioside, dulcoside A, steviolbioside) on silica gel (pre-washed with methanol and dried at 100 °C for 15 min) with ethyl acetate - methanol - acetic acid 3:1:1 over 60 mm. Detection under white light after immersion in ß-naphthol reagent (2 g in 180 mL ethanol with 12 mL 50 % sulfuric acid) and heating at 120 °C for 5 min. Quantitative absorption measurement at 500 nm after derivatization. LOD was 10 ng/band and LOQ 30 ng/zone. Using the calibration curve method the LOQ was reduced to 12 ng/band via peak height and 20 ng/band via peak area. The calculated expected tolerance range over the whole procedure inclusive sample preparation considered recovery rates at 3 different concentration levels (0.02, 0.13, and 0.20 %) in milk-based matrix. The accuracy (recovery tolerance limit of 92-120 %), repeatability (3.1-5.4 %) and intermediate precision (4.0-8.4 %) were highly satisfying, exemplarily shown for stevioside in milk-based matrix. ANOVA was successfully passed to prove the working range. With the newly developed and validated HPTLC method, steviol glycosides in Stevia leaves, Stevia formulations, and food products were investigated.
Marine Drugs 12 (5), 2633-2667 (2014). The saponin-enriched isobutanol-soluble fraction of a prepurified ethanol – water 7:3 extract of the viscera (all intern organs after removal of the body wall) of Holothuria lessoni Massin & al. (2009) was submitted to high performance centrifugal partition chromatography (HPCPC), before direct MS injection. For the monitoring of the HPCPC, TLC of subfractions on silica gel with the lower phase of chloroform – methanol – water 7:13:8. Detection under UV light and by spraying with 15 % sulfuric acid in ethanol followed by heating at 110 °C for 15 min, and evaluation in daylight, saponins are hued in maroon/dark purple. At least 39 new saponins and 36 reported triterpene glycosides (including holothurins, argusides and leucospilotasides), containing different aglycones and sugar moieties, were identified in the viscera of this sea cucumber.
J. Ethnopharmacol. 189, 186-193 (2016). HPTLC fingerprinting of salannin in the leaves of neem (Azadirachta indica A. Juss.; Meliaceae) on silica gel with ethyl acetate – dichloromethane – acetic acid – formic acid – water 100:25:10:10:11. The plates were allowed to dry at 100 °C for 5 min and then derivatized with Natural Product reagent (NPR) (1 g diphenyl borinic acid amino ethyl ester in 200 mL of ethyl acetate), followed by heating at 100 °C for 2–3 min and then dipped into anisaldehyde-sulfuric acid reagent (1 mL p-anisaldehyde, 10 mL sulfuric acid, 20 mL acetic acid in 170 mL methanol) and dried at 120 °C for 5 min. Qualitative identification under UV light at 254 or 366 nm.
Planta Med. 83, 1011-1019 (2017). The subfractionation on silica gel column of a diethyl ether fraction of a hydromethanolic extract of Lamium album aerial parts was controlled by TLC on silica gel with ethyl acetate – formic acid – water 20:2:3. Detection was performed at UV light after derivatization with 1 % Natural Product Reagent A. From the last subfraction, caffeic acid and tiliroside were isolated. The same TLC monitoring was applied for the fractionation of the butanol fraction of the extract. Some fractions and their subfractions were purified on dextran gel (Sephadex LH-20), and monitored by TLC on silica gel with ethyl acetate – formic acid – acetic acid – water 100:11:11:26, with the same detection. These subfractions contained caryoptoside and other iridoids, glucomartynoside, lamiusides and other phenylpropanoid derivatives.
J. Chromatogr. 258, 258- 261 (1983). TLC of numerous steroids and triterpenoids on silica. Detection with a saturated aq. solution of sulfosalicylic acid or with a saturated solution of picrylsulfonlc acid in glacial acetic acid, followed by heating at 120 °C for 5 minutes. Detection limit 300 ng.
Planta Med. 50, 485-488 (1984). Isolation of iridoid glucosides from a crude methanol extract of swertia japonica by PLC on silica with benzene - ethyl acetate - methanol 6:4:2 (five runs) and with chloroform - methanol - water 10:3:1 (lower layer). Detection by UV. Also OPLC of the permethylated derivatives on silica with chloroform - ether 1:1. Solvent systems suitable for other types of terpenoid glycosides.