Pharm. Res. 38, 127-140 (2021). HPTLC of [11C]metoclopramide in plasma, kidney, urine and liver extracts on silica gel with ethyl acetate - ethanol - 25 % ammonium hydroxide 16:4:1. Detection using a multisensitive phosphor screen. The hRF value for [11C]metoclopramide was 60. The method allowed kinetic analysis in different organs after radiotracer injection in mice.

Biochem. Biophys. Res. Commun. 525, 61-66 (2020). HPTLC of complete precursors of glycosylphosphatidylinositol (GPI) in Gpi8 deficient strains of Candida albicans on silica gel with chloroform - methanol - water 4:4:1. Metabolic labeling was performed using [2-3H]-myo-inositol. Detection of radiolabeled glycolipid intermediates by scanning for 5 min.

Pharmaceutical Methods 1(1), 39-43 (2010). TLC on silica gel with acetonitrile - chloroform 5:6. The hRf value of irbesartan was 27 and of hydrochlorothiazide 45. Densitometric evaluation at 270 nm. The method was linear in the range of 200-1000 ng/band for irbesartan and 200-600 ng/band for hydrochlorothiazide. The drug was subjected to different stress conditions (acid, base, oxidation, thermal, photolytic). All the degradation product were well resolved from the main drug. The proposed method is stability-indicating and suitable for routine analysis of fixed dose formulation in presence of degradation products.

J. Planar Chromatogr. 17, 449-453 (2004). In thin-layer radiochromatography the high sensitivity of the phosphor imaging analyzer can be further increased by multiple reading of the image plates, because the latent image is not lost quantitatively in the reading process. Summing of the chromatograms obtained in successive readings results in increased signal-to-noise ratio. Thus, by use of this approach either the exposure time can be shortened or higher sensitivity can be achieved. TLC of a14C-labeled test substance in rat serum on silica gel with chloroform - n-hexane - ethanol - ammonia 75:15:9:1. Detection by radioluminography.

and Pterocephalus hookeri (Clarke) Hoeck by thin-layer chromatography) (Chinese). Chinese J. of Ethnopharm. 7 (7), 19-21 (2013). The roots of the grasses Nardostachys chinensis Batal. and Pterocephalus hookeri (Clarke) Hoeck are herbal Tibetan drug for treating epidemic disease, long fever and dysentery. During investigation of the medicinal plants growing in the Qinghai and Tibetan Plateau the following identification method for the two drugs was developed. TLC on silica gel 1) for Nardostachys chinensis Batal., a) with cyclohexane – ethyl acetate 5:1 and b) with petroleum ether (60-90 °C) – acetone 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight. With mobile phase a) 3 zones were separated and with b) 6 zones; 2) for Pterocephalus hookeri (Clarke) Hoeck, a) with benzene – ethyl acetate 16:7 and b) with petroleum ether (60-90 °C) – acetone 3:2, all detection under UV 366 nm. With both mobile phases three blue fluorescent zones were detected.

J. Planar Chromatogr. 19, 42-47 (2006). TLC of 14C metabolites of denaverin (2,2-diphenyl-2-(2-ethylbutoxy)acetic acid-2-(dimethylamino)-ethyl ester) on silica gel with chloroform - cyclohexane - methanol - ammonia 100:70:30:3. Detection with autoradiographic films after exposure for one week. Quantitation after scraping the adsorbent from the plate followed by determination of radioactivity by LSC.

Food Chem. 177, 441-446 (2015). HPTLC of radiolabelled bovine casein-derived peptide Met-Lys-Pro (14C-MKP) and its fragments Met-Lys (MK), Lys-Pro (KP) and Lys (K) in plasma on silica gel with acetonitrile - acetic acid - water 2:1:1. Quantitative determination of the amount of intact 14C-MKP in plasma by radioactivity measurement using Bio-Imaging. The hRF values of MKP, MK, KP and K were 50, 52, 34 and 28, respectively.

Biochem. Biophys. Res. Commun. 487, 702-708 (2017). HPTLC of the reaction products of different 14C-labeled substrates of the mevalonate pathway (MVA, MVA-5-P, MVA-3-P, MVA-5-PP) and the recombinant Flavobacterium johnsoniae protein on silica gel with n-propanol – 28 % ammonia – water 6:3:1. The distribution of radioactivity on the TLC plate was detected using a suitable image analyzer.