Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      128 012
      Different glycolipids in sperm from different freshwater fishes – A high-performance thin-layer chromatography/electrospray ionization mass spectrometry study
      Kathrin ENGEL*, V. DZYUBA, B. DZYUBA, J. SCHILLER (*Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstr. 16–18, 04107 Leipzig, Germany, kathrin.engel@medizin.uni-leipzig.de)

      Rapid Commun. Mass Spectrom. 34, 8875 (2020). HPTLC of glycolipids in sperm from different fish species on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35. Detection by dipping into primuline (dissolved in acetone - water 4:1). Further analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and electrospray ionization ion trap (ESI-IT)MS.

      Classification: 11e
      127 021
      Development of a targeted HPLC-ESI-QqQ-MS/MS method for the quantification of sulfolipids from a cyanobacterium, selected leafy vegetables, and a microalgae species
      Judith FISCHER, M. TREBLIN, T. SITZ, S. ROHN* (*Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany, rohn@tu-berlin.de)

      Anal. Bioanal. Chem. 413, 1941-1954 (2021). HPTLC of sulfoquinovosylmonoacylglycerol (1) and sulfoquinovosyldiacylglycerol (2) in a cyanobacterium (Arthrospira sp.), a microalgae preparation (Chlorella vulgaris) and selected leafy vegetables (spinach, basil) on silica gel with chloroform - methanol - acetic acid - water 425:75:50:18. Detection by dipping into  5 % copper sulfate in 8 % phosphoric acid, followed by heating at 160 ºC for 30 min.

      Classification: 11e
      126 022
      The conserved arginine residue in all siglecs is essential for Siglec-7 binding to sialic acid
      A. YOSHIMURA, R. HATANAKA, H. TANAKA, K. KITAJIMA, C. SATO* (*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan, chi@agr.nagoya-u.ac.jp)

      Biochem. Biophys. Res. Commun. 534, 1069-1075 (2021). HPTLC of gangliosides in human and mouse siglecs on silica gel with chloroform - methanol - 0.2 % calcium chloride 60:25:4. Immunostaining by dipping into cyclohexane containing 0.1 % poly (isobutyl methacrylate) for 1 min, blocked by incubation in PBS containing 1 % BSA at room temperature for 1 h, incubated with premixture of Sig7EcFc WT or R124A and anti-hIgG + M + A-HRP in PBS containing 0.1 % BSA or S2-566 (anti-diSia antibody) in PBS containing 1 % BSA at room temperature for 2 h, and washed 5 times with PBS. Horseradish peroxidase activity was detected by chemiluminescence. Sig7EcFc corresponds to extracellular domain of Siglec-7 fused to the human IgG1 Fc region.

      Classification: 11e
      126 023
      Galabiosylceramide is present in human cerebrospinal fluid
      H. AKIYAMA*, M. IDE, T. YAMAJI, Y. MIZUTANI, Y. NIIMI, T. MUTOH, H. KAMIGUCHI, Y. HIRABAYASHI (*RIKEN Center for Brain Science, Wako, Saitama, 351-0198, Japan, hisako.akiyama@riken.jp)

      Biochem. Biophys. Res. Commun. 536, 73-79 (2021). HPTLC of ceramide dihexosides (lactosylceramide and its structural isomer, galabiosylceramide) in human cerebrospinal fluid on silica gel with chloroform - methanol - water 65:25:3. Detection by spraying with orcinol reagent, followed by heating at 120 ºC for 10 min.

      Classification: 11e
      126 024
      The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase
      S. SAH, S. SHEFALI, A. YADAV, P. SOM, S. KOMATH* (*Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, USA, sskomath@mail.jnu.ac.in)

      Biochem. Biophys. Res. Commun. 525, 61-66 (2020). HPTLC of complete precursors of glycosylphosphatidylinositol (GPI) in Gpi8 deficient strains of Candida albicans on silica gel with chloroform - methanol - water 4:4:1. Metabolic labeling was performed using [2-3H]-myo-inositol. Detection of radiolabeled glycolipid intermediates by scanning for 5 min.

      Classification: 11e
      102 024
      High-performance thin-layer chromatography/mass spectrometry for rapid analysis of neutral glycosphingolipids
      M. MIYAZAKI, A. YONESIGE, J. MATSUDA, Y. KURODA, N. KOJIMA, A, SUZUKI* (*Tokai University, Institute of Glycoscience, Hiratsuka, Kanagawa 259-1292, Japan; akmszk@tokai-u.jp)

      J. AOAC Int. 91, 1218-1226 (2008). HPTLC of glycosphingolipids on silica gel with chloroform - methanol - water 60:35:8 or 65:25:4. Detection by spraying with orcinol reagent followed by heating at 110 °C. Detection with HPTLC/MS by direct coupling of HPTLC to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry showed to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids.

      Classification: 11e
      122 025
      High-performance thin-layer chromatography coupled with electrospray ionization tandem mass spectrometry for identifying neutral lipids and sphingolipids in complex samples
      Carmen JARNE*, M. SAVIRON, M. LAPIEZA, L. MEMBRADO, J. ORDUNA, J. GALBAN, R. GARRIGA, G. MORLOCK, V. CEBOLLA (*Instituto de Carboquímica, ICB-CSIC, C/ Miguel Luesma, 4, 50018 Zaragoza, Spain, vcebolla@icb.csic.es)

      J. AOAC Int. 101, 1993-2000 (2018). HPTLC of neutral lipids in fatty acid methyl ester (FAME)-derived biodiesel (1) and sphingolipids in human plasma (2) on LiChrospher or silica gel with a 4-step gradient based on t-butyl methyl ether, dichloromethane, and n-heptane for (1) and a 7-step gradient based on methanol and dichloromethane for (2). Detection of neutral lipids by dipping into a methanolic primuline solution (0.02 g/100 mL), followed by fluorescence recording at 366/>400 nm. Detection of sphingolipids by absorbance measurement at 190 nm. Zones of interest were eluted into an electrospray ionization-ion trap MS (ESI-MS) using a TLC-MS interface. _x000D_

      Classification: 4e, 11c, 11e
      78 050
      Replacement of chloroform throughout glycosphingolipid isolation
      D. HEITMANN, M. LISSEL, R. KEMPKEN, J. MÜTHING*, (*Inst. Cell Cult. Technol., Univ. Bielefeld, P.O. Box 10031, D-33501 Bielefeld, Germany)

      Biomed. Chromatogr. 10, 245-250 (1996). Description of methods for glycosphingolipid extraction in excellent yield without the need for using toxic chloroform. TLC of lipid extracts on silica with various solvent systems. Detection by spraying with different reagents. Quantification by densitometry at 550 nm and 650 nm. Determination of sialic acid by HPLC. Calculation of the rank correlation coefficient. Comparison of the alternative solvent mixtures and chloroform in yields.

      Classification: 3d, 11e