Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 050
      Glycolipids isolation and characterization from natural source: A review
      A. DAKU*, S. AL-MHANNA, R. BAKAR, A. NURUL (*School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, 16150, Malaysia,

      J. Liq. Chromatogr. Relat. Technol. 45, 165-173 (2022). Review of different methods for the extraction and characterization of glycolipids, including chromatographic methods, such as TLC for the analysis of microbial glycolipids, marine source glycolipids, plant glycolipids and animal glycolipids.

      Classification: 1b, 11e
      130 147
      Globotriaosylceramide-related biomarkers of Fabry disease identified in plasma by high-performance thin-layer chromatography – densitometry – mass spectrometry
      C. JARNE, L. MEMBRADO, M. SAVIRÓN, J. VELA, J. ORDUNA, R. GARRIGA, J. GALBÁN, V. L. CEBOLLA* (*Institute of Carbon Chemistry, Spanish National Research Council (CSIC), Saragossa, Spain;

      J Chromatogr A 1638, 461895 (2021). Samples were sphingolipid-rich fractions of unproteinated blood plasma from healthy humans or from Fabry’s disease patients, as well as standards of sphingomyelin (SM) and of globotriaosylceramides (Gb3 = ceramide trihexosides), and related compounds (lyso-ceramide trihexosides, lactosyl ceramide, glucosyl ceramide). HPTLC on silica gel (Lichrosphere with spherical particles) by automated multiple development with a 9-step gradient, starting with pure methanol and ending with dichloromethane – methanol 9:1. Visualization and densitometry under UV 190 nm. Derivatization for Gb3 and derivatives (but not for SM) by immersion into orcinol solution (0.2 %, with sulfuric acid 10 %), followed by 15 min heating at 100 °C and by densitometry under visible light 550 nm. Bands of interest were directly eluted with methanol from underivatized plates into an ion-trap MS, through the oval head of a TLC-MS interface (with stainless steel frit to remove silica gel particles). Two different ionization processes were used: (A) electrospray ionization (ESI, capillary voltage 4 kV, endplate offset voltage -0.5 kV, nebulizer pressure 40 psi, drying gas 9 mL/min at 350 °C); (B) atmospheric pressure chemical ionization (APCI, capillary voltage 2–3 kV, current intensity 4.5 µA, nebulizer pressure 45 psi, drying gas 5 mL/min at 350 °C; vaporization at 450 °C). Full MS spectra were recorded up to m/z 1500 in positive ion mode. The relative ion intensities were used to quantify the detected species. Previous to this study, the precision of the elution head positioning was tested on Gb3 standard zones, comparing 3 positions for analyte elution: from the centre and from each higher or lower side of the band. The same main m/z peaks were observed in the 3 positions, but in different proportions. This was explained by the presence of coeluting Gb3 subclasses (the ceramide moiety CM being either saturated, mono-unsaturated fatty acyl with a slightly higher migration distance, or polar hydroxyl fatty acyl with the opposite effect on migration) and of coeluting Gb3 isoforms (the hexoside moiety consisting of glucose and/or galactose units). This resulted in the broadening and partial splitting of the standard band. In the plasma samples, 19 molecular species of Gb3 were identified (depending on the CM, the sugar isoforms being undistinguishable by MS): 5 with a saturated CM, 7 with two additional double bonds on the CM, 7 with a methylated CM. In case of Fabry’s disease, most Gb3 species with saturated CM were highly increased, whereas other species were decreased.

      Classification: 4e, 11c, 11e, 32f
      128 012
      Different glycolipids in sperm from different freshwater fishes – A high-performance thin-layer chromatography/electrospray ionization mass spectrometry study
      Kathrin ENGEL*, V. DZYUBA, B. DZYUBA, J. SCHILLER (*Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstr. 16–18, 04107 Leipzig, Germany,

      Rapid Commun. Mass Spectrom. 34, 8875 (2020). HPTLC of glycolipids in sperm from different fish species on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35. Detection by dipping into primuline (dissolved in acetone - water 4:1). Further analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and electrospray ionization ion trap (ESI-IT)MS.

      Classification: 11e
      127 021
      Development of a targeted HPLC-ESI-QqQ-MS/MS method for the quantification of sulfolipids from a cyanobacterium, selected leafy vegetables, and a microalgae species
      Judith FISCHER, M. TREBLIN, T. SITZ, S. ROHN* (*Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany,

      Anal. Bioanal. Chem. 413, 1941-1954 (2021). HPTLC of sulfoquinovosylmonoacylglycerol (1) and sulfoquinovosyldiacylglycerol (2) in a cyanobacterium (Arthrospira sp.), a microalgae preparation (Chlorella vulgaris) and selected leafy vegetables (spinach, basil) on silica gel with chloroform - methanol - acetic acid - water 425:75:50:18. Detection by dipping into  5 % copper sulfate in 8 % phosphoric acid, followed by heating at 160 ºC for 30 min.

      Classification: 11e
      126 022
      The conserved arginine residue in all siglecs is essential for Siglec-7 binding to sialic acid
      A. YOSHIMURA, R. HATANAKA, H. TANAKA, K. KITAJIMA, C. SATO* (*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan,

      Biochem. Biophys. Res. Commun. 534, 1069-1075 (2021). HPTLC of gangliosides in human and mouse siglecs on silica gel with chloroform - methanol - 0.2 % calcium chloride 60:25:4. Immunostaining by dipping into cyclohexane containing 0.1 % poly (isobutyl methacrylate) for 1 min, blocked by incubation in PBS containing 1 % BSA at room temperature for 1 h, incubated with premixture of Sig7EcFc WT or R124A and anti-hIgG + M + A-HRP in PBS containing 0.1 % BSA or S2-566 (anti-diSia antibody) in PBS containing 1 % BSA at room temperature for 2 h, and washed 5 times with PBS. Horseradish peroxidase activity was detected by chemiluminescence. Sig7EcFc corresponds to extracellular domain of Siglec-7 fused to the human IgG1 Fc region.

      Classification: 11e
      126 023
      Galabiosylceramide is present in human cerebrospinal fluid
      H. AKIYAMA*, M. IDE, T. YAMAJI, Y. MIZUTANI, Y. NIIMI, T. MUTOH, H. KAMIGUCHI, Y. HIRABAYASHI (*RIKEN Center for Brain Science, Wako, Saitama, 351-0198, Japan,

      Biochem. Biophys. Res. Commun. 536, 73-79 (2021). HPTLC of ceramide dihexosides (lactosylceramide and its structural isomer, galabiosylceramide) in human cerebrospinal fluid on silica gel with chloroform - methanol - water 65:25:3. Detection by spraying with orcinol reagent, followed by heating at 120 ºC for 10 min.

      Classification: 11e
      126 024
      The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase
      S. SAH, S. SHEFALI, A. YADAV, P. SOM, S. KOMATH* (*Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, USA,

      Biochem. Biophys. Res. Commun. 525, 61-66 (2020). HPTLC of complete precursors of glycosylphosphatidylinositol (GPI) in Gpi8 deficient strains of Candida albicans on silica gel with chloroform - methanol - water 4:4:1. Metabolic labeling was performed using [2-3H]-myo-inositol. Detection of radiolabeled glycolipid intermediates by scanning for 5 min.

      Classification: 11e
      103 043
      Matching IR-MALDI-o-TOF mass spectrometry with the TLC overlay binding assay and its clinical application for tracing tumor-associated glycosphingolipids in hepatocellular and pancreatic cancer
      U. DISTLER, M. HÜLSEWIG, J. SOUADY, K. DREISEWERD, J. HAIER, N. SENNINGER, A. W. FRIEDRICH, H. KARCH, F. HILLENKAMP, S. BERKENKAMP, J. PETER-KATALINIC, J. MÜTHING* (*Institute of Medical Physics and Biophysics, University of Münster, 48149 Münster, Germany;

      Anal. Chem. 80, 1835-1846 (2008). HPTLC of neutral glycosphingolipids on silica gel with chloroform - methanol - water 120:70:17 and 120:85:20, both supplemented with 2 mM calciumchloride solution. Detection by treatment with orcinol. Quantitative determination by absorbance measurement at 544 nm (orcinol) and 630 nm (indolylphosphate). A TLC overlay assay was performed, the TLC immunodetection procedure used glycosphingolipid antibodies and toxins in conjunction with antitoxin antibodies.

      Classification: 11e