Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      126 022
      The conserved arginine residue in all siglecs is essential for Siglec-7 binding to sialic acid
      A. YOSHIMURA, R. HATANAKA, H. TANAKA, K. KITAJIMA, C. SATO* (*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan,

      Biochem. Biophys. Res. Commun. 534, 1069-1075 (2021). HPTLC of gangliosides in human and mouse siglecs on silica gel with chloroform - methanol - 0.2 % calcium chloride 60:25:4. Immunostaining by dipping into cyclohexane containing 0.1 % poly (isobutyl methacrylate) for 1 min, blocked by incubation in PBS containing 1 % BSA at room temperature for 1 h, incubated with premixture of Sig7EcFc WT or R124A and anti-hIgG + M + A-HRP in PBS containing 0.1 % BSA or S2-566 (anti-diSia antibody) in PBS containing 1 % BSA at room temperature for 2 h, and washed 5 times with PBS. Horseradish peroxidase activity was detected by chemiluminescence. Sig7EcFc corresponds to extracellular domain of Siglec-7 fused to the human IgG1 Fc region.

      Classification: 11e
      126 023
      Galabiosylceramide is present in human cerebrospinal fluid
      H. AKIYAMA*, M. IDE, T. YAMAJI, Y. MIZUTANI, Y. NIIMI, T. MUTOH, H. KAMIGUCHI, Y. HIRABAYASHI (*RIKEN Center for Brain Science, Wako, Saitama, 351-0198, Japan,

      Biochem. Biophys. Res. Commun. 536, 73-79 (2021). HPTLC of ceramide dihexosides (lactosylceramide and its structural isomer, galabiosylceramide) in human cerebrospinal fluid on silica gel with chloroform - methanol - water 65:25:3. Detection by spraying with orcinol reagent, followed by heating at 120 ºC for 10 min.

      Classification: 11e
      126 024
      The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase
      S. SAH, S. SHEFALI, A. YADAV, P. SOM, S. KOMATH* (*Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, USA,

      Biochem. Biophys. Res. Commun. 525, 61-66 (2020). HPTLC of complete precursors of glycosylphosphatidylinositol (GPI) in Gpi8 deficient strains of Candida albicans on silica gel with chloroform - methanol - water 4:4:1. Metabolic labeling was performed using [2-3H]-myo-inositol. Detection of radiolabeled glycolipid intermediates by scanning for 5 min.

      Classification: 11e
      102 024
      High-performance thin-layer chromatography/mass spectrometry for rapid analysis of neutral glycosphingolipids
      M. MIYAZAKI, A. YONESIGE, J. MATSUDA, Y. KURODA, N. KOJIMA, A, SUZUKI* (*Tokai University, Institute of Glycoscience, Hiratsuka, Kanagawa 259-1292, Japan;

      J. AOAC Int. 91, 1218-1226 (2008). HPTLC of glycosphingolipids on silica gel with chloroform - methanol - water 60:35:8 or 65:25:4. Detection by spraying with orcinol reagent followed by heating at 110 °C. Detection with HPTLC/MS by direct coupling of HPTLC to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry showed to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids.

      Classification: 11e
      122 025
      High-performance thin-layer chromatography coupled with electrospray ionization tandem mass spectrometry for identifying neutral lipids and sphingolipids in complex samples
      Carmen JARNE*, M. SAVIRON, M. LAPIEZA, L. MEMBRADO, J. ORDUNA, J. GALBAN, R. GARRIGA, G. MORLOCK, V. CEBOLLA (*Instituto de Carboquímica, ICB-CSIC, C/ Miguel Luesma, 4, 50018 Zaragoza, Spain,

      J. AOAC Int. 101, 1993-2000 (2018). HPTLC of neutral lipids in fatty acid methyl ester (FAME)-derived biodiesel (1) and sphingolipids in human plasma (2) on LiChrospher or silica gel with a 4-step gradient based on t-butyl methyl ether, dichloromethane, and n-heptane for (1) and a 7-step gradient based on methanol and dichloromethane for (2). Detection of neutral lipids by dipping into a methanolic primuline solution (0.02 g/100 mL), followed by fluorescence recording at 366/>400 nm. Detection of sphingolipids by absorbance measurement at 190 nm. Zones of interest were eluted into an electrospray ionization-ion trap MS (ESI-MS) using a TLC-MS interface. _x000D_

      Classification: 4e, 11c, 11e
      78 050
      Replacement of chloroform throughout glycosphingolipid isolation
      D. HEITMANN, M. LISSEL, R. KEMPKEN, J. MÜTHING*, (*Inst. Cell Cult. Technol., Univ. Bielefeld, P.O. Box 10031, D-33501 Bielefeld, Germany)

      Biomed. Chromatogr. 10, 245-250 (1996). Description of methods for glycosphingolipid extraction in excellent yield without the need for using toxic chloroform. TLC of lipid extracts on silica with various solvent systems. Detection by spraying with different reagents. Quantification by densitometry at 550 nm and 650 nm. Determination of sialic acid by HPLC. Calculation of the rank correlation coefficient. Comparison of the alternative solvent mixtures and chloroform in yields.

      Classification: 3d, 11e
      103 017
      On-Line Overpressure Thin-Layer Chromatographic Separation and Electrospray Mass Spectrometric Detection of Glycolipids
      W. CHAI*, Christine LETEUX, A. LAWSON, M. STOLL (*MRC Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Watford Road, Harrow, Middlesex HA1 3UJ, U.K.,

      Anal. Chem. 75, 118-125 (2003). Online TLC separation and electrospray mass spectrometry (TLC/ESI-MS) by direct linking of a commercial overpressure TLC instrument, OPLC 50, and a Q-TOF mass spectrometer. Separation on silica gel with dichlormethane - methanol - water 60:35:8. A sensitivity of 5 pmol of glycosphingolipid was readily demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/MS production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis, although this may result in a minor reduction in TLC resolution. Following solvent development, separated components on the TLC plates can be detected in the conventional way by nondestructive staining or UV absorption or fluorescence and can be stored for on-line TLC/ESI-MS analysis at a later stage without reduction in mass spectrometric detection sensitivity and chromatographic resolution. Aspects for further improvement of OPLC instrumentation include use of narrower TLC plate dimensions and refined design of the eluate exit system.

      Classification: 4e, 11e
      122 071
      Vulnerability of anthocyanins to the components of a thin-layer chromatographic system and comprehensive screening of anthocyanes in alimentary products
      E. ?ATA, A. FULCZYK, Teresa KOWALSKA*, M. SAJEWICZ (*Dep. of General Chem. & Chromatogr., Inst. of Chem., Univ. of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland,

      J. Chromatogr. A 1572, 137-144 (2018). Development of a method for the analysis of anthocyanes within the foodstuffs of plant origin by TLC on RP-18 phase (which ensures mixed-mode retention mechanism with the localized adsorption on the non-bonded silanols) with acetic acid as the mobile phase component, using two anthocyanins (cyanin and keracyanin) and two anthocyanidins (pelargonidin and delphinidin) as phytochemical standards. By triple development, distinct and symmetrically shaped chromatographic zones were obtained. Further analysis of the products of hydrolytic degradation of the test anthocyanins by MS. Identification of the hydrolytically split fractions by using the p-aminobenzoic acid reagent. Investigation of the calibration curves for cyanin, keracyanin, pelargonidin and delphinidin, and the respective LOD and LOQ values. Application of the method to identify and quantify cyanin, keracyanin, pelargonidin and delphinidin in selected alimentary products (syrups, juices and herbal infusions).

      Classification: 11e, 30b