J. Liq. Chrom. & Rel. Technol. 22, 1493-1502 (1999). Two- or three-dimensional TLC and HPTLC of lipids (i. a. phospholipids + cerebrosides + free sphingoid bases and their methylated analogs; ceramides and DAG species) on silica gel with 1. chloroform - methanol 100:7, and 2. with petroleum ether - ether - acetic acid 400:600:1; if separation of TAGs from esterified fatty acids and cholesteryl esters is required, a third-dimensional separation can be performed with hexane - ether - acetic acid 80:20:1. Visualization by spraying with Dittmer and Lester reagent for the detection of phospholipids, followed by heating at 180°C for 1 min.

J. Proteome Res. 14, 567-577 (2015). HPTLC of (1) lipids and (2) cholesterol from urinary exosomes on silica gel predeveloped in chloroform - methanol 1:1. (1) was developed with with two mobile phases: chlorofom - ethanol - triethylamine - water 30:35:35:8, and hexane - ether 200:9; (2) with chloroform - acetone 19:1. Detection by dipping in 10 % copper sulfate followed by heating at 185 °C for 5 min.

Chromatogr. 686, 155-157 (1994). Description of a TLC developing tank designed to give optimal saturation conditions, with the title compounds as example, resulting in highly reproducible chromatograms, on silica with chloroform - methanol - 0.2% aqueous CaCl2 60:37:8. Visualization by orcinol reagent.

J. Liq. Chrom. & Rel. Technol. 20, 1149-1157 (1997). Preparative TLC of gangliosides (for the removal of the non-polar lipids) on silica gel with chloroform - methanol - 0.3% calcium chloride solution. After elution HPTLC on silica gel with chloroform - methanol - 0.1 M sodium lactate 11:8:2. Visualization by spraying with orcinol reagent (0.5% solution in 20% sulfuric acid).

Rapid Commun. Mass Spectrom. 29, 1288-1296 (2015). HPTLC of sphingomyelins (1), phosphatidylcholines (2) and phosphatidylethanolamines (3) on silica gel with chloroform - methanol - water - acetic acid 30:15:2:4 for the first step and acetone - acetonitrile - chloroform 5:4:2 for the second step. Detection by spraying with 0.5 % amido black 10B stain in 1 M NaCl. The method was coupled with matrix-assisted laser desorption/ionisation mass spectrometry for the analysis of (1) and (2) extracted from 5,6-dimethylxanthenone-4-acetic acid-treated xenograft tumours.

Anal. Biochem. 225, 24-27 (1995). Glycosphingolipids separated by TLC on silica with chloroform - methanol - 0.2% CaCl2 60:35:8 were transferred to a polyvinylidene difluoride membrane by TLC blotting. Analysis by SIMS. Detection limit about 1 µg. The major advantage of this method is that glycosphingolipids can be analyzed structurally without purification by repeated column chromatography.

Anal. Chem. 75, 118-125 (2003). OPLC of acidic glycolipids (e.g. GM3, GD3, GT1b, chondroitin sulfate disaccharides, heparan sulfate tetrasaccharide) on silica gel. Under optimized conditions, a sensitivity of 5 pmol of glycosphingolipid was demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/M production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis. Conventional HPTLC on silica gel with chloroform - methanol - water 60:35:8. Detection by nondestructive staining or UV absorption or fluorescence.

J. Food Comp. Anal. 44, 45-55 (2015). HPTLC of gangliosides based on the degree of sialylation in the milk fat globule membrane of whole milk on silica gel with chloroform - methanol - 28 % ammonia - water 60:35:7:3. Bands containing gangliosides were excised and glangliosides extracted for LC-MS analysis.