Rapid Commun. Mass Spectrom. 29, 1288-1296 (2015). HPTLC of sphingomyelins (1), phosphatidylcholines (2) and phosphatidylethanolamines (3) on silica gel with chloroform - methanol - water - acetic acid 30:15:2:4 for the first step and acetone - acetonitrile - chloroform 5:4:2 for the second step. Detection by spraying with 0.5 % amido black 10B stain in 1 M NaCl. The method was coupled with matrix-assisted laser desorption/ionisation mass spectrometry for the analysis of (1) and (2) extracted from 5,6-dimethylxanthenone-4-acetic acid-treated xenograft tumours.

Anal. Biochem. 225, 24-27 (1995). Glycosphingolipids separated by TLC on silica with chloroform - methanol - 0.2% CaCl2 60:35:8 were transferred to a polyvinylidene difluoride membrane by TLC blotting. Analysis by SIMS. Detection limit about 1 µg. The major advantage of this method is that glycosphingolipids can be analyzed structurally without purification by repeated column chromatography.

Anal. Chem. 75, 118-125 (2003). OPLC of acidic glycolipids (e.g. GM3, GD3, GT1b, chondroitin sulfate disaccharides, heparan sulfate tetrasaccharide) on silica gel. Under optimized conditions, a sensitivity of 5 pmol of glycosphingolipid was demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/M production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis. Conventional HPTLC on silica gel with chloroform - methanol - water 60:35:8. Detection by nondestructive staining or UV absorption or fluorescence.

J. Food Comp. Anal. 44, 45-55 (2015). HPTLC of gangliosides based on the degree of sialylation in the milk fat globule membrane of whole milk on silica gel with chloroform - methanol - 28 % ammonia - water 60:35:7:3. Bands containing gangliosides were excised and glangliosides extracted for LC-MS analysis.

Fat Sci. Technol. 97, 453-454 (1995). Analytical TLC of fatty acids (i. a. cyclopropenoic acid, vernolic acid) on silica with ether - hexane 2:8; 3:7. In addition preparative TLC of mixed fatty acids for separation into oxygenated and nonoxygenated fractions. Detection by spraying with dichlorofluorescein and visualization under UV.

Anal. Chem 75, 6728-6731 (2003). Fluorometric method for the determination of quantities of gangliosides ranging from pico- to nanomoles. HPTLC of sugars (D-(+)-glucose, D(+)-galactose, D(+)-frucose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-neuramic acid) and gangliosides (GM1, asialoGM1, GD1a, GT1b) on silica gel, prewashed with methanol - chloroform 1:1, with chloroform - methanol - 0.2% CaCl2 (aq) 60:35:8 as solvent system in a well-saturated TLC chamber. Detection by spraying with 18% hydrochloric acid thoroughly drying at 40°C in an drying oven and heating for 12 min at various temperatures (from 50 to 180°C). The fluorescence (UV 365 nm) of each sample was greatly dependent on the heating temperature. Calibration curves for gangliosides were obtained by HPTLC and an image-analyzing system equipped with a CCD camera and they showed a high linearity in a wide range from 47 pmol to 4.5 nmol. New and simple procedure.

PLoS ONE 8(3), e57908 (2013). Feline SD (Sandhoff disease) fibroblasts GM2 SV3 and human TSD (Tay-Sachs disease) glial cells were transfected to express hybrid subunits H1 and H2 of beta-hexosaminidase A (Hex) and grown for 18 h in medium containing 4.7 µg/mL NBD-GM2 (2-nitro1,3-benzoxadiazol fluorescent derivative of GM2 ganglioside, substrate of Hex) and 50 µM CBE (conduritol-B-epoxide). To check the transfection, HPTLC of glycosphingolipids, extracted according to Folch’s method into acidic and neutral glycolipids, on silica gel first with chloroform – acetone 1:1, followed by chloroform – methanol – 0.2% CaCl2 55:45:10; the separated gangliosides (NBD-GM2 and its breakdown NBD-products) were quantified by a fluor image analyzer (Storm Imager). Total ganglioside fractions obtained through ion-exchange chromatography from transfected SD lysates were incubated with NBD-GM2 and separated on HPTLC, with the same first mobile phase followed by chloroform – methanol – water 65:25:4; the reaction product NBD-GM3 appeared only when GM2 activator protein was added during the incubation.

J. Planar Chromatogr. 8, 362 - 365 (1995). HPTLC of macrophage and brain gangliosides on silica with chloroform - methanol- 0.02% aqueous calcium chloride 60:40:9. Visualization by spraying with resorcinol hydrochloride and heating at 110°C for 15 min. After the above described chromatographic separation the gangliosides were transferred from the silica gel layer to the PVDF membrane with the ganglioside electrotransfer technique. This enables easy determination of ganglioside structure by the application of specific antibodies.