Biochem. Biophys. Res. Commun. 470, 168-174 (2016). HPTLC of glycosphingolipid during the evaluation of enzyme activities of human Gb3/CD77 synthase supplemented with UDP-[14C]Gal on silica gel with chloroform – methanol – water 11:9:2. Detection of radiolabeled reaction products by exposure to phosphor screens for 8 weeks in -80 °C, followed by scanning. In parallel staining was used to facilitate identification of bands on the phosphor screens by spraying the plates with orcinol (0.2 %) in 3 M aqueous sulfuric acid, followed by heating at 110 °C for 10 min.

(German). Fat Sci. Technol. 97, 229-235 (1995). TLC of membrane phosphatides (sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, cardiolipin) on silica with chloroform - methanol - acetic acid - water 30:15:4:2. Detection by spraying with 0.05% rhodamin 6G in acetone. TLC of acetylated diglycerides on silica impregnated with 10% aqueous silver nitrate solution and chloroform - methanol 99:1 or chloroform - acetic acid 19:1. In addition TLC on RP-18 with acetone - acetonitrile - chloroform 5:4:2. Densitometry.

J. Agric. Food Chem. 64, 1245-1255 (2016). Preparative HPTLC of gangliosides in the frontal lobe of piglets on silica gel with chloroform – methanol – calcium chloride 50:42:11. Fractions were subjected to a series of reactions to obtain sialic acid, glucose, galactose, N-acetyl-galactosamine, and fatty acid derivatives.

Anal. Biochem. 223, 232-238 (1994). Description of a method for purifying glycosphingolipids and phospholipids by using TLC blotting. Separation of glycosphingolipids by two-dimensional TLC and detection with primuline reagent. Transfer of the separated spots by TLC blotting to a polyvinylidene difluoride membrane, and extraction; monitoring by TLC.

J. Planar Chromatogr. 30, 460-466 (2017). HPTLC of cholesterol oleate, glycerol trioleate, squalene, β-sitosterol, linoleic acid, glycosylsterol (β-sitosterol glucoside), glycosylceramide, protocatechuic acid and quercetin 4ʹ-O-β-d-glucopyranoside (2) on silica gel with chloroform ‒ methanol 17:3. Detection by dipping into a solution of copper(II) sulfate (10 %), phosphoric acid (8 %), and methanol (5 %) in water, followed by heating at 150 °C for 10 min. Quantitative determination by absorbance measurement at 546 nm. Linearity was between 25 and 1000 ng/zone. LOD and LOQ were between 50 and 125 ng/band and 125 and 250 ng/band, respectively. The intermediate precision was <2 % (n=6). Recovery ranged from 106.5 % to 112.1 %.

Anal. Biochem. 227, 195-200 (1995). TLC mapping of the title compounds on NH2-bonded silica with chloroform - methanol - 1% diethylamine 50:47:15 for the 1st dimension, transporting the acid glycosphingolipids on this plate to a silica plate by developing with chloroform - methanol - NH3 2:5:3 after NH2-plate placed face to face on top of silica plate, and developing the plate with chloroform - methanol - 0.2% CaCl2 6:4:1 for the 2nd dimension. With this method it is possible to compare detailed membrane constituents, including minormolecular species with regard to acidic glycosphingolipids.

Biochem. Biophys. Res. Commun. 497, 1082-1088 (2018). TLC of lipids extracted from the different intraerytrocytic stages of P. falciparum labelled with nitrobenzoxadiazole-ceramide or nitrobenzoxadiazole-DHceramide as fluorescent precursors and treated or not with tamoxifen. TLC on silica gel with chloroform – methanol – water 40:10:1 for neutral and zwitterionic lipids and propanol – ammonia – water 15:1:1 for acidic lipids. The effect of tamoxifen on glycosphingolipid biosynthesis was evaluated by fluorescent precursor incorporation.

Anal. Chim. Acta 311, 57-62 (1995). Two-dimensional HPTLC of gangliosides in human plasma on silica with 1) chloroform - methanol - 0.2 % aqueous CaCl2 5:4:1, and 2) propanol - NH3 3:2 for both directions, resp. Derivatization with resorcinol reagent. Quantification by image processing; GM4 was used as internal standard.