Anal. Chem 75, 6728-6731 (2003). Fluorometric method for the determination of quantities of gangliosides ranging from pico- to nanomoles. HPTLC of sugars (D-(+)-glucose, D(+)-galactose, D(+)-frucose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-neuramic acid) and gangliosides (GM1, asialoGM1, GD1a, GT1b) on silica gel, prewashed with methanol - chloroform 1:1, with chloroform - methanol - 0.2% CaCl2 (aq) 60:35:8 as solvent system in a well-saturated TLC chamber. Detection by spraying with 18% hydrochloric acid thoroughly drying at 40°C in an drying oven and heating for 12 min at various temperatures (from 50 to 180°C). The fluorescence (UV 365 nm) of each sample was greatly dependent on the heating temperature. Calibration curves for gangliosides were obtained by HPTLC and an image-analyzing system equipped with a CCD camera and they showed a high linearity in a wide range from 47 pmol to 4.5 nmol. New and simple procedure.

PLoS ONE 8(3), e57908 (2013). Feline SD (Sandhoff disease) fibroblasts GM2 SV3 and human TSD (Tay-Sachs disease) glial cells were transfected to express hybrid subunits H1 and H2 of beta-hexosaminidase A (Hex) and grown for 18 h in medium containing 4.7 µg/mL NBD-GM2 (2-nitro1,3-benzoxadiazol fluorescent derivative of GM2 ganglioside, substrate of Hex) and 50 µM CBE (conduritol-B-epoxide). To check the transfection, HPTLC of glycosphingolipids, extracted according to Folch’s method into acidic and neutral glycolipids, on silica gel first with chloroform – acetone 1:1, followed by chloroform – methanol – 0.2% CaCl2 55:45:10; the separated gangliosides (NBD-GM2 and its breakdown NBD-products) were quantified by a fluor image analyzer (Storm Imager). Total ganglioside fractions obtained through ion-exchange chromatography from transfected SD lysates were incubated with NBD-GM2 and separated on HPTLC, with the same first mobile phase followed by chloroform – methanol – water 65:25:4; the reaction product NBD-GM3 appeared only when GM2 activator protein was added during the incubation.

J. Planar Chromatogr. 8, 362 - 365 (1995). HPTLC of macrophage and brain gangliosides on silica with chloroform - methanol- 0.02% aqueous calcium chloride 60:40:9. Visualization by spraying with resorcinol hydrochloride and heating at 110°C for 15 min. After the above described chromatographic separation the gangliosides were transferred from the silica gel layer to the PVDF membrane with the ganglioside electrotransfer technique. This enables easy determination of ganglioside structure by the application of specific antibodies.

Ghica Voda 41A, Iassy, Romania): Immunoassay detection of gangliosides by specific antibodies. CBS 94, 11-13 (2005). HPTLC of gangliosides extracted from tissues on silica gel with chloroform - methanol - 0.2% aqueous CaCl2 11:9:2 over 60 mm. Immunoassay detection by dipping in polyisobutylmethacrylate solution and bovine serum albumine solution, followed by immersion in anti-body containing supernatant or patient’s sera at 4° C overnight. After washing with phosphate-buffered saline detection of Mab binding by stepwise incubation with biotinylated chain-specific anti-mouse immunoglobulin, followed by steptavidin-horsradish peroxidase complex. Visualisation with chloro-4-naphtol reagent. Immuno detection is better than chemical derivatization with resorcinol-HCl reagent and has advantages over detection on ELISA microtiter plates.

Biochem. Biophys. Res. Commun. 470, 168-174 (2016). HPTLC of glycosphingolipid during the evaluation of enzyme activities of human Gb3/CD77 synthase supplemented with UDP-[14C]Gal on silica gel with chloroform – methanol – water 11:9:2. Detection of radiolabeled reaction products by exposure to phosphor screens for 8 weeks in -80 °C, followed by scanning. In parallel staining was used to facilitate identification of bands on the phosphor screens by spraying the plates with orcinol (0.2 %) in 3 M aqueous sulfuric acid, followed by heating at 110 °C for 10 min.

(German). Fat Sci. Technol. 97, 229-235 (1995). TLC of membrane phosphatides (sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, cardiolipin) on silica with chloroform - methanol - acetic acid - water 30:15:4:2. Detection by spraying with 0.05% rhodamin 6G in acetone. TLC of acetylated diglycerides on silica impregnated with 10% aqueous silver nitrate solution and chloroform - methanol 99:1 or chloroform - acetic acid 19:1. In addition TLC on RP-18 with acetone - acetonitrile - chloroform 5:4:2. Densitometry.

J. Agric. Food Chem. 64, 1245-1255 (2016). Preparative HPTLC of gangliosides in the frontal lobe of piglets on silica gel with chloroform – methanol – calcium chloride 50:42:11. Fractions were subjected to a series of reactions to obtain sialic acid, glucose, galactose, N-acetyl-galactosamine, and fatty acid derivatives.

Anal. Biochem. 223, 232-238 (1994). Description of a method for purifying glycosphingolipids and phospholipids by using TLC blotting. Separation of glycosphingolipids by two-dimensional TLC and detection with primuline reagent. Transfer of the separated spots by TLC blotting to a polyvinylidene difluoride membrane, and extraction; monitoring by TLC.