59th Indian Pharmaceutical Congress C-176, 267, (2007). HPTLC of tylophorine, kaempferol, alpha-amyrin, and quercetin in an alcoholic extract from Tylophora indica on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Densitometry at 366 nm. The method was found suitable for the estimation of quercetin in plant drugs and their formulations.

Asian J. Chem. 1, 509-513 (2008). HPTLC of andrographolide in Andrographis paniculata homoeopathic mother tincture on silica gel with chloroform - methanol 7:1. Quantitative determination by absorbance measurement at 225 nm. The standard mother tincture was found to contain 21.9 mg/100 mL andrographolide, where as 6 commercial samples contained varying amounts of andrographolide ranging from 4.89-54.62 mg/100 mL.

Chinese J. Pharm. Anal. 28 (9), 1554-1556 (2008). TLC of scalreol on silica gel with n-hexane – ethyl acetate – formic acid 32:16:1. Detection by spraying with vanillin – sulfuric acid – ethanol 1:1:18. Quantification by densitometry at 520 nm. Linearity was between 10 and 50 µg/zone with a determination coefficient of 0.9994. Recovery was 100.1 % (n = 6, %RSD = 0.9). Repeatability (%RSD, n = 6) was 2.1 % within plate and 2.3 % plate-to-plate.

International Journal of Pharma and Bio Sciences 1(4), 339-345 (2010). TLC on silica gel (plates pre-washed with methanol) with chloroform - toluene - methanol - glacial acetic acid 50:20:30:1. The hRf value of s-amlodipine was 33 and of nebivolol 48. Densitometric evaluation at 271 nm. The linearity range was 500-2500 ng/zone for nebivolol and 250-1250 ng/zone for s-amlodipine. The method was suitable for routine analysis of the formulation.

Acta Chromatographica 22 (1), 91-97 (2010), DOI:10.1556/AChrom.22.2010.1.7. TLC on silica gel with chloroform – methanol – toluene 4:3:3. The hRf value was 77 and 29 for cefuroxime axetil and potassium clavulanate, respectively. Quantification by densitometry at 225 nm. The linearity was in the range of 0.5–2.5 and 2–10 µg/band, respectively. Application of the method for analysis of the drugs in a pharmaceutical formulation with a recovery of 100.1 % for cefuroxime axetil and 99.9 % for potassium clavulanate.

J. Chinese Modern Med. & Pharm. 20 (31), 54-56 (2013). Paracetamol and chlorphenamine maleate granule are an anti cold medicine effective for cold induced nasal congestion, headache, sore throat, fever, etc. Artificial bezoar is one of the main ingredients and bilirubin is the key effective component in the medicine. For quality control, TLC of the extracts and bilirubin on silica gel with isooctane – ethyl acetate – glacial acetic acid 11:5:3, detection under white light. Quantitative determination of bilirubin by UV spectrophotometry. Demonstration by applying to real life samples from three pharmaceutical factories indicated that the method was suited for the quality control of the medicine.

J. Chromatogr. Sci. 54 (5), 842-846 (2016). Development of a new chromatographic method for direct enantioresolution of (RS)-baclofen by ligand-exchange TLC, adopting two different approaches: (A) on plates prepared by mixing the ligand exchange reagents (LER) with silica gel slurry, development with different achiral solvents or solvents without chiral additive; (B) on silica gel without chiral selector and the LER consisting of Cu(II)–L-amino acid complex as chiral mobile phase additive. Preparation of LERs by using Cu(II) acetate and four L-amino acids (L-tryptophan, L-histidine, L-proline and L-phenylalanine). Detection by exposure to iodine vapor. Study of the effect of temperature and the mole ratio of Cu(II)-to-amino acid on enantioresolution. L-tryptophan proved to be a good ligand using a common mobile phase in each case.

Chromatographia 18, 253-259 (1984). HPTLC of antiarrhythmic drugs on silica with benzene - ethyl acetate - methanol - triethylamine 7:6:1:1 and of chloramphenicol on silica with heptane - chloroform - methanol 6:12:3. Densitometry by absorbance at 265 nm or by fluorescence for the former and by absorbance at 280 nm for the latter. Method for monitoring of therapeutic drugs in blood serum. Comparison with EMIT and HPLC.