Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.17-19 (2004). HPTLC on silica gel with 1) toluene - acetone - ethanol - ammonia 20:25:3:2; 2) n-hexane - ethyl acetate - glacial acetic acid 15:5:1; 3) n-hexane - ethyl acetate - ammonia 20:20:1. Detection 1) by spraying with 5 % potassium iodobismuthate solution; 2) by spraying with 1 % potassium permanganate in diluted sulfuric acid followed by heating at 120 ºC, and under UV 360 nm. Identification by fingerprint technique. Determination of the content of aconitine by comparison with standard.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.19-20 (2004). HPTLC on silica gel with petroleum ether (30 - 60 ºC) - formic acetate - formic acid 15:5:1, at 11 ºC and humidity of 40 %. Detection by exposing to ammonia vapors. Identification by fingerprint technique and comparison with the standards.

CBS 95, 14-15 (2005). HPTLC-AMD of lipids from drug formulations with an 11-step gradient based on ethyl acetate. Detection by dipping in an aqueous copper sulfate solution followed by heating at 150 °C for 30 min. Quantitative determination by absorbance measurement at 675 nm, evaluation of peak area with calibration according to Hill kinetics.

J. AOAC Int. 88, 1555-1561 (2005). HPTLC of chloramphenicol on silica gel in a horizontal developing chamber (36 applications per plate) using n-hexane -ethyl acetate 7:13. Quantitative determination by absorbance measurement at 280 nm. Mean recovery was 95.8 %, and the coefficient of variation was 5.8 %. The detection limit was 3 ng, and the quantitation limit 10 ng.

J. Pharm. Biomed. Anal. 37, 817-821 (2005). CO2 extracts of Harpagophytum procumbens root was evaluated by HPLC and HPTLC for harpagoside contents. HPTLC on silica gel with ethyl acetate - methanol - water 77:15:8 in saturated ADC chamber. Detection by dipping into anisaldehyde reagent followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 509 nm. The linearity range was 0.04-0.40 mg/mL. The HPTLC method was less time consuming than HPLC, needing almost no sample pre-treatment. 15 different CO2-extracts of the plant were analysed.

J. Planar Chromatogr. 18, 282-284 (2005). TLC and HPTLC of horseradish and mustard samples on silica gel with iso-propanol - 25 % ammonia 9:1 containing different volumes of water (1, 2, 3, or 5 parts). After development the compounds were visualized under UV light at 254 nm or by exposure to iodine vapor.

J. Pharm. Biomed. Anal. 39, 801-804 (2005). HPTLC on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The hRf values of stavudine (SV), lamivudine (LV) and nevirapine (NV) were 25, 67 and 87 respectively. The method was linear within the range of 0.01-0.06 mg/mL, 0.05-0.30 mg/mL and 0.06-0.40 mg/mL for SV, LV, and NV respectively, recovery rates were between 98.2 and 99.9 %. The HPTLC method was compared with the UV and HPLC methods. Accuracy, precision and ruggedness of the method were established.

Indian J. Pharm Sciences 67 (6), 687-690 (2005). HPTLC of sparfloxacin extracted with dichloromethane from plasma. A standard solution was prepared in methanol - dichloromethane 1:1. HPTLC on silica gel with chloroform - toluene - methanol - diethylamine 44:15:2:1. Quantitative determination by absorbance measurement at 301 nm. The method had a linearity range of 80-200 ng/spot with an average recovery of 89.17 %.