J. AOAC Int. 101, 1397-1401 (2018). HPTLC of sudan red dyes, namely Sudan orange G (1), Sudan red G (2), Sudan I (3), Sudan II (4), Sudan III (5) and Sudan IV (6) in spices and spice mixtures on RP-18 acetonitrile – methanol – aqueous ammonia solution (25 %) 40:9:1. Quantitative determination using a flatbed scanner with a 16-bit resolution. The hRF values for (1) to (6) were 54, 48, 57, 35, 26 and 17, respectively. Linearity was between 20 and 500 ng/zone for (1) to (6). LOD and LOQ were 17 and 35 ng/zone for (1), 11 and 21 ng/zone for (2), 14 and 31 ng/zone for (3), 12 and 24 ng/zone for (4), 18 and 42 ng/zone for (5) and 16 and 37 ng/zone for (6), respectively.

J. Planar Chromatogr. 31, 361-365 (2018). TLC of (±)-terbutaline on silica gel with chiral ligand-exchange reagent (LER). L-(‒)-Trp, L-(‒)-Phe, and L-(‒)-His were chosen as chiral selectors. Copper(II) acetate (2 mM) and L-amino acid (4 mM) solutions were prepared in water ‒ methanol 17:1 and mixed to be used as LER. In terms of resolution, best enantioseparation was obtained with acetonitrile ‒ methanol ‒ chloroform ‒ water 10:4:3:3 using L-Trp as chiral selector. The hRF values for (-) and (+)-isomers were 20 and 56, respectively.

CBS 81, 2-5 (1998). The performance and reliability of result of both procedures is comparable - HPTLC being slightly better than HPLC. For the assay of phospholipids in pure substances, pharmaceutical products and lecithin HPTLC is more cost efficient than HPLC by a factor of 2.5.

CBS 86, 4-5 (2001) HPTLC of 3-methoxy-4-amino azo meta sulphonic acid (MAMASA) on silica gel with n-butanol - diethylamine - ammonia - methanol 9:5:5:2 with chamber saturation for 5 min followed by drying at 40 °C. Quantitative determination by absorbance measurement at 254 nm.

CBS 88, 4-6 (2002). HPTLC of rhubarb extract on silica gel (prewashed with isopropanol) with acetone - water - formic acid 18:1:1 or toluene - acetone - formic acid 3:6:1 over 75 mm after partial chamber saturation. Detection by 1) dipping in a 1 % solution of vanillin in ethanol and heating at 50 °C for 5 min followed by exposing to HCl atmosphere, 2) spraying with iron (III) chloride 3 % in methanol, 3) spraying with 4-dimethylaminocinnamaldehyde 1 % in ethanol - HCl (37 %) 1:1, 4) spraying with natural products reagent 1 % in methanol. Documentation under white light and at 366 nm.

CBS 93, 5-7 (2004). HPTLC of N,N-diethylethane-1,2-diamine in metoclopramide finished products on silica gel with 32 % ammonia - methanol - dichloromethane 3:15:80 over 40 mm with chamber saturation. Detection by dipping in 0.2 % ethanolic ninhydrin solution for 1 s, followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 480 nm and evaluation of peak area with polynomial regression. The correlation coefficient of the calibration curve is 0.9995, the residual standard deviation 2.19 %. Intermediate precision is 1.65 %. Recovery for 0.2-1.0 % impurity is 100.5 %. Limit of quantitation is 0.05 % impurity.

Indian J. Pharm. Sci. 66 (3), 278-282 (2004). HPTLC on silica gel with chloroform - acetonitrile - toluene - acetate buffer (pH 6.0) 50:40:10:3. Quantitative determination by absorbance measurement at 266 nm. The method was validated in terms of linearity, accuracy, precision, and specificity. The limit of detection and the limit of quantification were found to be 50 ng/spot and 100 ng/spot respectively. A simple, precise, accurate and rapid HPTLC method has been developed and validated for the simultaneous determination of cefuroxime axetil and probenecid in combined dosage form.

Ghica Voda 41A, Iassy, Romania): Immunoassay detection of gangliosides by specific antibodies. CBS 94, 11-13 (2005). HPTLC of gangliosides extracted from tissues on silica gel with chloroform - methanol - 0.2% aqueous CaCl2 11:9:2 over 60 mm. Immunoassay detection by dipping in polyisobutylmethacrylate solution and bovine serum albumine solution, followed by immersion in anti-body containing supernatant or patient’s sera at 4° C overnight. After washing with phosphate-buffered saline detection of Mab binding by stepwise incubation with biotinylated chain-specific anti-mouse immunoglobulin, followed by steptavidin-horsradish peroxidase complex. Visualisation with chloro-4-naphtol reagent. Immuno detection is better than chemical derivatization with resorcinol-HCl reagent and has advantages over detection on ELISA microtiter plates.