J. Planar Chromatogr. 25, 338-343 (2012). HPTLC of fluvoxamine in the presence of degradation products on silica gel with ethyl acetate - toluene - methanol - ammonia 14:4:2:1. Quantitative determination by absorbance measurement at 254 nm. The hRf of fluvoxamine was 63. Linearity was in the range of 100-1000 ng/zone. Limits of detection and quantification were 10 and 100 ng/zone. The intermediate/inter-day/intra-day precision was below 0.9 % (n=6). Recovery was between 96.9 and 100.1 %.

J. Planar Chromatogr. 25, 463-467 (2012). HPTLC of the enantiomers of the beta-blockers (bisoprolol, metoprolol, and propranolol) and a beta-2-agonist (salbutamol) on silica gel impregnated with Cu(II) complexes of L-threonine, L-serine, and L-tartaric acid, with different mobile phases. Limits of detection were within the range 0.19-0.26 µg for enantiomers of the selected compounds. L-Ser proved to be a very good ligand for exchange and successful enantio-resolution using common mobile phases.

J. Planar Chromatogr. 25, 138-144 (2012). HPTLC of diosmin (1) and hesperidin (2) on silica gel with ethyl acetate - methanol - water - acetic acid 25:2:2:1. Quantitative determination by absorbance measurement at 330 nm. Linearity was in the range of 100-3000 ng/zone for (1) and 250-7500 ng/zone for (2). The method did not show any statistically significant deviation when compared with a validated HPLC.

J. Chromatogr. A 1223, 142-146 (2012). Hydrophilic vitamins play an important role in human health, and their lack or excess produces specific diseases. Development of a method for the analysis of these compounds for monitoring their content in pharmaceuticals and food. Application of TLC in the simultaneous analysis of hydrophilic vitamins with the optimized conditions towards different chemical characteristics of analytes. Due to the difficulty of separation of the structural analogues of these hydrophilic vitamins in one chromatographic run, taking the advantage of TLC to perform 2-D separations by using stationary phase gradient of silica gel and cellulose, achieving the highest resolution by combining two systems with different selectivity. For the gradient TLC the silica gel and cellulose plates were cut in narrow strips each of 1.5 x 9 cm and connected face-to-face. Development with methanol - benzene - formic acid 6:4:1. Detection under UV 254 nm. The method has been used for identifying the water-soluble vitamins in alcoholic extracts of Hippophae rhamnoides and of Ribes nigrum.

CBS 107, 2-4 (2011). HPTLC of chloroform extracts from cleaning swabs on silica gel with toluene - ethyl acetate 3:2 after 10 min chamber saturation. Densitometric evaluation for identification of substances by spectra recording at 200-350 nm, quantification with 3-level calibration. Detection by immersion in methanol - sufuric acid 9:1 and heating at 105 °C for 5 min. Cleaning validation by HPTLC (i.e. determination of residues of hormonal ingredients) can be achieved with the required accuracy. The method is simple and quick and the quantitative results are precise.

of Northern Pharmacy 9 (4), 4-5 (2012) Chongderi pills are a herbal traditional Mongolian medicine for treating diarrhea, invigorating the circulation of blood, stimulating the menstrual flow, and dissipating blood stasis, etc. For quality control, TLC on silica gel 1) for Radix aucklandiae, with benzene – methanol 27:1, detection by spraying with 1% vanillin in sulfuric acid – ethanol, detection under daylight; 2) for Rhizoma acori tatarinowii, with petroleum ether (60-90 º) – ethyl acetate 9:1, detection by exposure to iodine vapors, viewing under daylight; 3) for safflower carthamus, with ethyl acetate – formic acid – water – methanol 35:10:15:2, detection under daylight. Identification by comparison with the standards.

J. Planar Chromatogr. 25, 133-137 (2012). HPTLC of thiocolchicoside (1) and aceclofenac (2) in combined dosage form on silica gel with methanol - chloroform - water 48:1:1. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) and (2) were found to be 70 and 83, respectively. Linearity was in the range of 30-180 ng/band for (1) and 750-4500 ng/band for (2). Recovery was in the range of 98.7-101.2 % for both.

J. AOAC Int. 96, 1175-1188 (2013). Review of different methods for antifungal screening. Latest developments in HPTLC, where the correlation of bioactivity and quantification was needed to evaluate the antifungal potential. TLC-bioautography was presented as a simple way to screen antifungal activities and two visualization approaches for this technique were described, as well as recent TLC antifungal bioautography applications.