Food Chem. 134184 (2023). HPTLC of 45 berry cultivars belonging to the nine species strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry on silica gel with toluene - ethyl acetate - formic acid - water 15:30:5:3. Detection by dipping into a 0.5 % solution of 2-aminoethyl diphenylborinate in ethyl acetate, followed by drying and dipping into a 5 % solution of PEG 400 in dichloromethane. Qualitative analysis under UV light at 366 nm. 

Food Chem. 133108 (2022). HPTLC of 135 baobab samples (Adansonia digitata) from different agroecological regions on silica gel with toluene - ethyl acetate - methanol - formic acid - water 60:50:25:3:5. Detection by dipping into aniline diphenylamine o-phosphoric acid reagent, p-amino benzoic acid reagent, or p-anisaldehyde sulfuric acid reagent, followed by heating at 120 °C for 5 min. Qualitative analysis under UV light at 254 nm. fluorescence detection at 366 nm and white light illumination. 

Food Chem. 134216 (2023). Review of emerging techniques for the analysis of animal-derived food, including HPTLC methods for authenticity and origin tracing of honeys. In particular, methods for the determination of phenols in honey was cited using principal component analysis as discrimination model. 

Food Chem. 133610 (2022). HPTLC of hormonal active compounds in 68 different botanicals on silica gel with ethyl acetate - toluene - formic acid - water 16:4:3:2. The plate was neutralized by spraying with citrate phosphate buffer (6 g/L citric acid monohydrate and 10 g/L disodium hydrogen phosphate in double-distilled water, adjusted to pH 12 by solid sodium hydroxide). To overcome diffusion caused by long bioassay incubation, zone fixation was achieved by coating with polyisobutyl methacrylate (0.25 % Degalan in n-hexane), followed by drying. The prepared yeast cell culture was piezoelectrically sprayed onto the plates, followed by incubation at 30 °C for 4 h. The substrate solution (2 mg 4-methyl umbelliferyl-β-D-galactopyranoside in 100 μL dimethyl sulfoxide and 3 mL citrate buffer) was piezoelectrically sprayed, followed by incubation at 37 °C for 1 h, dried, and documented by fluorescence light detection at 366 nm. The resulting NP-HPTLC–UV/Vis/FLD–pYAVAS–FLD bioassay allowed the detection of androgens, antiandrogens, false-positive antiandrogens, and synergists in complex mixtures.

Food Chem. 132941 (2022). HPTLC of monosaccharides glucose, galactose and rhamnose in Abroma augusta mucilage on silica gel with acetone - butanol - water 5:4:1. Detection by spraying with orcinol-sulfuric acid solution, followed by heating at 90 °C for 5 min. 

Marine Drugs 17(3), 148 (2019). Samples were ethyl acetate extracts of seagrass Amphibolis antarctica (Cymodoceaceae), and of algae: Austrophyllis harveyana (Kallymeniaceae), Carpoglossum confluens, Cystophora harveyi, C. monilifera, C. pectinata and C. subfarcinata, Myriodesma integrifolium, Sargassum lacerifolium (Sargassaceae), Codium fragile subsp. tasmanicum (Codiaceae), Ecklonia radiata (Lessoniaceae), Hypnea valida, Rhodophyllis membaneacea (Cystocloniaceae), Hormosira banksii (Hormosiraceae), Perithalia caudata (Sporochnaceae), Phyllospora comoasa, Scytothalia dorycarpa (Seirococcaceae), Plocamium dilatatum (Plocamiaceae), and epiphytic brown algae. HPTLC on silica gel (pre-washed with methanol and heated 30 min at 100 °C) with n-hexane – ethyl acetate – acetic acid 15:9:1. Derivatization by immersion: A) into anisaldehyde – sulfuric acid reagent, followed by 10 min heating at 105 °C, for the detection of steroids and terpenes; B) into DPPH• (0.2 % in methanol), followed by 30 min incubation in the dark, for the detection of antioxydants; C) into Fast Blue B solution (0.1 % in 70 % ethanol) for detection of phenols (with alkylresorcinols detected as dark purple zones on colorless background). Effect-directed analyses were performed directly on the plates. D) α-Amylase inhibition assay by immersion into enzyme solution, incubation 30 min at 37 °C, immersion into substrate solution (starch 2 % in water), incubation 20 min at 37 °C and immersion into Gram’s iodine solution for detection (inhibition zones appear blue on white background). E) Acetylcholinesterase (AChE) inhibition assay (after neutralization by immersion into phosphate buffer) by immersion into enzyme solution, incubation 30 min at 37 °C, immersion into substrate solution (α-naphthyl acetate) and into dye reagent (Fast Blue Salt B). Densitometry through automated scanning, quantification expressed as equivalents to the respective standards used for calibration curves: A) β-sitosterol (LOQ 1.6 µg/band), B) gallic acid (LOQ 60 ng/band), D) acarbose (LOQ 173 µg/band), E) donepezil (LOQ 96 µg/mL). Alkylresorcinols were detected as antioxydant in C. harveyi and C. pectinata (hRF 88), and in C. subfarcinata (hRF 72, 81, 88). Enzymatic inhibitors in C. fragile were considered as a flavone (hRF 65) and a terpenoid (hRF 77), due to their absorption curves (densitometric scan in range 200-400 nm).

J. AOAC Int. 104, 19-25 (2022). HPTLC of xipamide (1) and triamterene (2) on silica gel with toluene - methanol - ethyl chloride - acetic acid 35:10:5:1. Quantitative determination by absorbance measurement at 300 nm. The hRF values for (1) and (2) were 52 and 37, respectively. Linearity was between 0.3 and 7.0 µg/zone for (1) and 0.3 and 12.0 µg/zone for (2). Inter-day and intra-day precisions were below 2 % (n=3). The LOD and LOQ were 47 and 141 ng/zone for (1) and 75 and 228 ng/zone for (2), respectively. Mean recovery was 100.2 % for (1) and 100.8 % for (2).

J. AOAC Int. 104, 1188-1195 (2021). HPTLC of syringic acid (1) and vanillic acid (2) in the roots of Ricinus communis and aerial parts of Euphorbia hirta on silica gel with toluene - ethyl acetate - formic acid 14:5:1. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) and (2) were 50 and 60, respectively. Linearity was between 2 and 10 µg/zone for both (1) and (2). Inter-day and intra-day precisions were below 2 % (n=3). The LOD and LOQ were 1518 and 5066 ng/zone for (1) and 331 and 1104 ng/zone for (2), respectively. Mean recovery was 99.4 % for (1) and 98.7 % for (2).