J. Planar Chromatogr. 22, 157-161 (2009). Safed musli is the common name for Chlorophytum borivilianum, whereas shatavari is Asparagus racemosus, both of the liliaceae family. HPTLC of saponins and sapogenins on silica gel with chloroform - methanol - water 32:25:5 for saponins and with petroleum ether - ethyl actate 4:1 for sapogenins. Detection of saponins by spraying with anisaldehyde reagent and with Ehrlich’s reagent, followed by densitometric analysis at 620 and 510 nm. Detection of sapogenins by spraying with Liebermann Burchard reagent, followed by densitometric analysis at 580 nm.

Abstract No. 9148, IHCB (2009). HPTLC of gallic acid in Emblica officinalis (an important constituent of Triphala, a popular ayurvedic formulation) on silica gel with toluene - ethyl acetate - formic acid 10:10:3. The hRf value of gallic acid was 45. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 150-530 ng/spot. The extract was found to contain 7.5 mg/g of gallic acid.

J. Planar Chromatogr. 21, 271-275 (2008). HPTLC of capsaicin and its degradation products (after acidic and alkaline hydrolysis, oxidation and thermal degradation) on silica gel with toluene - ethyl acetate 3:2. Quantitative determination by absorbance measurement at 280 nm. The limit of detection and quantification was 10 and 60 ng/band, respectively.

Biomed. Chromatogr. 22 (11), 1237-1242 (2008). TLC of dl-penicillamine on silica gel 1) impregnated with L-tartaric acid; 2) impregnated with (R)-mandelic acid; and 3) with no impregnation but with chiral modifier added to the mobile phase. The mobile phases used were 1) acetonitrile - methanol - water 5:1:1; 3) ethyl acetate - methanol - water 3:1:1; and 2) acetonitrile - methanol - 0.5 % aqueous L-tartaric acid (pH 5) - glacial acetic acid 70:10:11:7. Detection by exposure to iodine vapors. The limit of detection of both was 120 ng/zone by use of L-tartaric acid (either by impregnation or added to mobile phase), and 110 ng/zone by use of (R)-mandelic acid. (R)-mandelic acid was found to be successful as a chiral impregnating reagent.

60th Indian Pharmaceutical Congress PA-217 (2008). HPTLC of oleanolic acid in total methanolic extract of fruits of Randia dumetorum lam. on silica gel with toluene - ethyl acetate - acetic acid 70:30:1 in a twin trough chamber saturated for 10 min. Detection by treatment with 10 % sulphuric acid in methanol, followed by heating at 110 °C and immediate densitometric evaluation. Quantitative determination by absorbance measurement at 540 nm. The method was linear in the range of 50-500 ng/spot. Recovery was in the range of 99.4-100.8 %.

J. Planar Chromatogr. 22, 235-240 (2009). HPTLC of pesticides (clofentezine, neburon, chlorfenvinphos, lenacyl, trifluralin, thiram, procymidone, flufenoxuron, tralkoxydim, propaquizafop, dinoseb) and water samples (sample preparation by solid phase extraction) on silica gel with ethyl acetate - n-heptane 1:4 and 3:7 in a horizontal chamber. Quantitative determination by diode array densitometry in the range of 200 to 600 nm. The limit of detection was between 40 and 650 ng/spot and the limit of quantification was between 120 and 1920 ng/spot.

Br. root powder and extract. J. Planar Chromatogr. 22, 453-456 (2009). HPTLC of 2-hydroxy-4-methoxybenzaldehyde and biological extracts on silica gel with toluene - ethyl acetate - acetic acid 7:2:1 in a twin trough chamber saturated for 20 min. Quantitative determination by absorbance measurement at 277 nm.

Abstract No. 91334, IHCB (2009). Standardization of Tinospora cordifolia extract by HPTLC of syringic acid on silica gel with chloroform - methanol 8:1. The hRf value of syringic acid was 53. Quantitative determination by absorbance measurement at 254 nm.