J. Chinese Trad. Patent Med. 29 (4), 540-542 (2008). TLC of Tianbing Tiaodu capsule extracts on silica gel with cyclohexane - ethyl acetate 4:1. Detection 1) by spraying with vanillin reagent (5 % vanillin in sulfuric acid - ethanol 1:6) and heating until the zones were visualized; 2) under UV 365 nm. Identification by comparison of the chromatograms with that of the standard ferulic acid.

The case of Bifidobacterium adolescentis MB 239. J. Planar Chromatogr. 22, 321-325 (2009). HPTLC of glucose, fructose, galactose, lactose, raffinose, 1-kestose, nystose, and fructosyl-nystose on diol phase by automated multiple development with acetonitrile - acetone - water and acetonitrile - water. Preliminary isocratic developments were performed at 22-25 °C at 65-75 % relative humidity in a twin trough chamber. Detection by vaporization with 37 % hydochloric acid vapor for 30 min followed by dipping in 4-aminobenzoic acid. Quantitative determination by fluorescence measurement at 313 nm.

J. Planar Chromatogr. 22, 371-376 (2009). HPTLC of indolizidine alkaloids and extracts on silica gel and RP-18 (both prewashed with chloroform-methanol 1:1) with 11 mobile phases in a horizontal chamber without chamber saturation. The best resolution was achieved on silica gel with chloroform - methanol 20:1. Detection under UV 254 nm and after spraying with Dragendorff reagent. Quantitative determination of securinine and allosecurinine by absorbance measurement at 254 nm. The limit of detection and quantification was 11 and 36 ng/zone for securinine and 12 and 41 ng/zone for allosecurinine, respectively.

60th Indian Pharmaceutical Congress PG-251 (2008). HPTLC of glycyrrhetinic acid and piperine in haematinic herbomineral capsule formulation on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. Quantitative determination by absorbance measurement at UV 254 nm. The method was linear in the range of 0.8-2.4 ng/spot (glycyrrhetinic acid) and 10-50 ng/spot (piperine). Recovery was 96.3-98.6 %.

Phytochem. Anal. 20, 421-426 (2009). HPTLC of delta-9-tetrahydrocannabinol in the flowertops of Cannabis sativa on silica gel with chloroform with chamber saturation for 20 min. Quantitative determination by absorbance measurement at 206 nm. Derivatization by dipping in Fast Blue B solution for 5 s. The hRf value of delta-9-tetrahydrocannabinol was 47 and selectivity regarding matrix was given. Linearity was given between 50 and 500 ng/zone. The limit of quantification and detection was 50 and 10 ng/zone, respectively. The intra- and inter-day repeatability (%RSD, n = 9) were not higher than 5.0 %. Recovery was 85.8 % for delta-9-tetrahydrocannabinol in decarboxylated Cannabis samples. The method was shown to be comparable within a small degree of error (0.5 %) to results from a validated HPLC method.

Ind. J. Pharma. Sci. 71(1), 95-97 (2009). HPTLC of emtricitabine and tenofovir on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The calibration curve was linear between 200 and 1000 ng with a regression coefficient of 0.9995.

Abstract No. F-244, 61st IPC (2009). HPTLC of rabeprazole and itopride on silica gel with ethyl acetate - methanol - benzene - chloroform 2:4:3:1. The hRf value was 42 and 61 for rabeprazole and itopride, respectively. Quantitative determination by absorbance measurement at 276 nm. The method was linear in the range of 200-300 ng/band for rabeprazole and 1500-2250 ng/band for itopride.

Abstract No. F-315, 61st IPC (2009). HPTLC for pravastatin on silica gel with ethyl acetate - toluene - acetonitrile - formic acid 60:35:5:2. Quantitative determination by absorbance measurement at 237 nm. The method was linear in the range of 318-3816 ng/band. Recovery was 99.9-101.2 %. Stability tests showed that degradation products resulting under acid stress conditions were well resolved from the main component.