J. Planar Chromatogr. 22, 101-107 (2009). HPTLC of aceclofenac on silica gel (prewashed with methanol) with toluene - ethyl acetate - methanol - glacial acetic acid 70:20:20:1 in a saturated twin trough chamber. Quantitative determination by absorbance measurement at 270 nm. Linearity was between 0.8 and 14 µg/mL. Average relative residual at each calibration point combined with accuracy and precision were compared using the parameter rank approach. The 1/ x 2 weighted regression model provided best estimates with small relative residuals and the best accuracy and precision.

J. Planar Chromatogr. 22, 19-23 (2009). HPTLC of 26 UV filter substances and their photodegradation products on LiChrospher silica gel (prewashed with methanol) by automated multiple development with mixtures of tert-butyl ether - n-hexane. Detection under UV light at 254 and 366 nm. Bioassay by immersion of plates for 1 s in a suspension of Vibrio fischeri bacteria followed by evaluation in dark.

Abstract No. 9192, IHCB (2009). HPTLC of betulinic acid in hydro alcoholic extracts of Nelumbo nucifera rhizome and seed, on silica gel with chloroform - methanol - formic acid 49:1:1. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 420 nm. The method was linear in the range of 2-10 ng/spot, recovery was 98.1-98.4 %.

J. AOAC Int. 92, 691-697 (2009). HPTLC of dialkyl phosphate standards (dimethyl phosphate, diethyl phosphate, dimethyl thiophosphate, diethyl thiophosphate and dibutyl phosphate as internal standard) on amino phase, prewashed with methanol, with dichloromethane in a twin trough chamber. Quantitative determination by fluorescence measurement at 366/>400 nm. The limit of quantification was between 0.8 and 1.4 ng/zone. Fluorescence enhancement was achieved by dipping the plate into a 50 % solution of paraffin oil in n-hexane, increasing the sensitivity and resulting in an LOQ of 0.5-0.6 ng/zone.

Ind. J. Pharma. Sci. 8(4), 198-201(2009). HPTLC of nebivolol hydrochloride and valsartan on silica gel with ethyl acetate - methanol - acetic acid 12:2:1 in a twin trough chamber saturated for 15 min. Quantitative determination by absorbance measurement at 240 nm for valsartan and 280 nm for nebivolol hydrochloride. The method was found to be linear in the range of 600-1400 ng/band for valsartan 1200-2800 ng/band for nebivolol. The sample were subjected to different stress conditions (acid, alkali, oxidation, photolysis, thermal) and all degradation products were well separated from the main compounds.

Abstract No. 9162, IHCB (2009). HPTLC of glycyrrhizin in methanolic (70 %) extracts of Glycyrrhiza glabra on silica gel with chloroform - methanol - water 130:72:15. Quantitative determination by absorbance measurement at 254 nm and at 420 nm after spraying with anisaldehyde reagent. The method was linear in the range of 0.8-3.8 µg/spot. The limit of detection and quantification was 0.16 and 0.52 µg/spot, respectively. Glycyrrhizin was used as bioactive marker for quality control.

Abstract No. F-269, 61st IPC (2009). HPTLC of hydrochlorothiazide and irbesartan on silica gel with acetonitrile - chloroform 5:6. The hRf value was 27 and 45 for irbesartan and hydrochlorothiazide, respectively. Quantitative determination by absorbance measurement at 270 nm. The sample was exposed to different stress conditions (acid, alkali, oxidative, photodegradation, thermal). Neither of the compounds showed degradation under thermal and photodegradation conditions, but both compounds showed significant degradation under acid, alkali and hydrolytic conditions. Degraded products were well resolved from the parent compounds.

60th Indian Pharmaceutical Congress PG-258 (2008). HPTLC of isovitexin in Passiflora incarnata raw material and extract on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. The plant was found to contain 0.087 % w/w of isovitexin.