CBS 115, 13-15 (2015). HPTLC of Java tea (Orthosiphon species) and standards rhamnazin, apigenin-4,5,7-trimethylether, 6-methoxyluteolin, luteolin tetramethylether, scutellarein tetramethylether, tangeretin,_x000D_ eupatorin, eupatorin-5-methylether, and sinensetin on silica gel with toluene – ethyl acetate – methanol 11:8:1 with chamber saturation for 20 min to the migration distance of 70 mm. Detection under UV 254 nm and 366 nm. Densitometric evaluation by absorbance measurement at 254 nm. Elution of zones with TLC-MS Interface into a single mass spectrometer. Together with the already established sinensetin, scutellarein tetramethylether, eupatorin, and eupatorin-5-methylether were determined as specific markers suitable for the identification of Orthosiphon aristatus (Blume) Miq. by HPTLC. The presence of these four markers in the flavonoid profile is specific for Java tea.

J. Food Comp. Anal. 45, 26-33 (2016). HPTLC of monoacylglycerol and free fatty acids in carrot seed oil on silica gel with hexane – diethyl ether – acetic acid 50:50:1. The zones were marked and scraped off the plate for positional distribution analysis by gas chromatography.

J. Chromatogr. A 1432, 140-144 (2016). 2-Aminoacetophenone (AAP) is closely correlated with the appearance of the sensory phenomenon of untypical aging off-flavor (UTA) in wine and is generally analyzed by GC/MS after being extracted from wines by liquid-liquid, solid-liquid or solid phase microextraction. Presentation of a rapid, selective and sensitive method for the determination of AAP in wine by HPTLC of the extracts, obtained by liquid-liquid extraction with t-butyl methyl ether followed by a basic cleanup, on amino layer with methylene chloride – toluene 7:3. Detection by dipping into n-hexane-paraffin solution and quantification by densitometry at 366/>400 nm using 2-amino-4-methoxyacetophenone as internal standard. The LOD and LOQ were 0.1 and 0.3 μg/L, respectively. The recovery was near 100 % for model, white and red wines, while AAP concentrations were >0.5 μg/L in UTA. The results indicated that the method enables the analysis of AAP in wines clearly below the odor thresholds and represents a rapid and convenient screening alternative to existing GC/MS methods.

J. Planar Chromatogr. 29, 195-202 (2016). HPTLC of naftopidil on silica gel with toluene – ethyl acetate – methanol – triethylamine 80:20:5:3. Quantitative determination by absorbance measurement at 254 nm. The hRF value was 52. Linearity was in the range of 500-2500 ng/zone. Intermediate precisions were below 1.5 %. The LOD and LOQ were 95 and 268 ng/zone. Average recovery was 100.0%.

Phytochemistry. 130, 22-46 (2016). The review describes 141 steroidal saponins and sapogenins from the genus Agave reported in the literature from 1970 to 2015. It is a comprehensive review of structures, methods of chemical profiling, including the application of TLC and 2D-TLC for the analysis of saponins and sapogenins, and HPTLC for the determination of hecogenin.

J. Planar Chromatogr. 29, 341-346 (2016). HPTLC of artemisinin in the seeds of Artemisia annua on silica gel with n-hexane ‒ ethyl acetate ‒ acetic acid 20:10:1. Detection by dipping into anisaldehyde reagent (glacial acetic acid ‒ sulfuric acid ‒ anisaldehyde 50:1:0.5) followed by heating at 110 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for artemisinin was 43. Linearity was between 200 and 1000 ng/zone. The intermediate precision was below 0.7 % (n=3). The LOD and LOQ were 30 and 80 ng/zone, respectively. Recovery ranged from 82.9 to 87.1 %.

J. of Chromatogr. A 1441, 126-133 (2016). Presentation of a method for the analysis of ergot alkaloids including an ammonium acetate buffered extraction step, followed by a fast liquid-liquid partitioning pre-cleaning and then planar solid phase extraction (pSPE). HPTLC on amino phase with methanol for separation of the ergot alkaloids from the remaining matrix and for focusing them in a single zone. Quantification after dipping the plate in n-hexane – paraffin solution for fluorescence enhancement. The LOD and LOQ was 0.07 and 0.24 mg/kg rye, respectively, expressed as ergocristine, which was well below the currently applied quality criteria limit for rye. The recovery was almost 100 % at relevant spiking levels for different rye flour samples. The pSPE–FLD method was fast, efficient and reliable for screening the total ergot alkaloid content in rye and it was a rapid alternative to the HPLC determination with summing up the individual alkaloids. Furthermore, HPTLC-MS additionally enables the identification of the ergot alkaloid composition by a single mass spectrum, when utilized as a fingerprint, offering an easy differentiation of Secale cornutum from different origins.

J. Planar Chromatogr. 29, 366-371 (2016). HPTLC of the diastereomeric amides of (RS)-etodolac on silica gel with acetonitrile – methanol – dichloromethane – water 12:2:2:1. Detection by exposure to iodine vapor. The hRf value for the first and second eluting diastereomers were 22 and 56, respectively.