Abstract No. F-235, 61st IPC (2009). HPTLC of olanzapine on silica gel with methanol - ethyl acetate 4:1. The hRf value was 35. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 100-600 ng/band. The method was suitable for analysis of formulations and in-house prepared mucoadhesive microemulsions.

60th Indian Pharmaceutical Congress PG-264 (2008). HPTLC of quercetin in acetone extracts of Psidium guajava leaves on silica gel with toluene - acetone - formic acid 38:10:5. Quantitative determination of quercetin by absorbance measurement at 364 nm. The correlation coefficient was 0.9847. There was a good correlation between peak area and corresponding concentration of quercetin. The proposed HPTLC method provided a good resolution of quercetin from other constituents present in acetone extract of tender leaves of Psidium guajava and can be used for the quantification of quercetin.

60th Indian Pharmaceutical Congress PA-223 (2008). HPTLC of curcumin, demethoxycurcumin and bis-demethoxycurcumin on silica gel with chloroform - methanol 19:1. The hRf values were 25, 38, and 61 for bis-demethoxycurcumin, demethoxycurcumin, and curcumin respectively. Quantitative determination by absorbance measurement at 420 nm. The method was linear in the range of 50-400 ng/spot (curcumin), 10-150 ng/spot (demethoxycurcumin), and 5-40 ng/spot (bis-demethoxycurcumin). Recovery was in the range of 99.2-100.5 % for all three compounds.

60th Indian Pharmaceutical Congress PG-349 (2008). HPTLC of 3H-4M-benzaldehyde (in crude plant material, extracts and dosage form of Hemidesmus indicus) on silica gel with toluene - ethyl acetate - methanol - acetic acid 15:3:1:1. Quantitative determination by absorbance measurement at 230 nm.

f.) Etting. J. Planar Chromatogr. 23, 104-107 (2010). TLC of (+)-catechin, ellagic acid, quercetin, and ferulic acid on silica gel with toluene - ethyl acetate - fomic acid 6:4:1 in a twin-trough chamber previously saturated for 30 min at 25 °C. Other mobile phase compositions were ethyl acetate - methanol - water 10:1:1, n-butanol - acetic acid - water 4:1:5 (upper layer), n-butanol - ethanol - water 20:5:11, n-butanol - acetic acid - water 6:2:1, toluene - ethyl acetate - formic acid 5:5:1 and 7:3:1, and toluene - ethyl acetate - methanol - formic acid 140:60:1:1. Quantitative determination by absorbance measurement at 320 nm. Detection by dipping in anisaldehyde reagent, followed by drying and heating at 110 °C for 5 min. Characteristic bands of (+)-catechin, ellagic acid, quercetin, and ferulic acid were observed at hRf 35, 41, 63 and 66, respectively. Precision (CV, n = 7) was 0.39, 0.44, 0.55 and 0.16 % and repeatability (CV, n = 7) 0.65, 0.95, 0.37 and 0.18 %, respectively. LOD was 54, 340, 48 and 78 ng/band, and LOQ 4.1, 6.5, 1.6 and 1.5 µg/band, respectively. Linear regression was 0.9993 (100-500 ng/band), 0.9883 (1000-5000 ng/band), 0.9989 (100-500 ng/band) and 0.9987 (100-500 ng/band), respectively.

Quim. Nova 33, 43-47 (2010). HPTLC of aflatoxin B1 in peanuts on silica gel with chloroform - acetone 99:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by image processing using the software ImageJ. Linearity was between 0.8 and 4.8 ng/zone. The intra-day and inter-day precisions had a RSD lower than 5.2 %. LOD was 0.4 ng/zone while LOQ was 1.2 µg/kg. The average recovery was 94.9 %. The proposed method is a simple, efficient and low cost tool for quantitative analysis of aflatoxin B1 in peanut samples.

IPA Convention, 2010, RA-PO 03. HPTLC of edaravone in samples of human plasma (purified by liquid-liquid extraction) on RP-18 with n-butanol - methanol - diethyl ether 1:8:1. The compound was well resolved with an hRf value of 81. Densitometric evaluation at 240 nm. The limit of detection and quantification was 25 ng and 150 ng respectively. The linearity was 600-2400 ng/band. Recovery (%) based on analysis of spiked sample was more than 65 %.

Abstract No. C-97, 61st IPC (2009). Chromatographic methods are reported for identification (TLC) and quantification (HPTLC) of withaferin-A in methanolic and chloroform extract of dried fruits of Withania coagulans. Chromatographic separation on silica gel with toluene - ethyl acetate - formic acid 5:5:1. The identification of withaferin-A in both chloroform and methanolic extracts was performed by comparison of hRf values and UV absorbance maxima (209 nm). Quantification was performed by absorbance measurement at 540 nm after spraying the developed plate with Liebermann-Burchard reagent. Methanolic extracts and chloroform extracts contained 3.67 mg/g and 2.10 mg/g of withaferin-A, respectively. No withaferin-A was found in hydroalcoholic extracts.