Indian J. Pharm. Sci. 66 (3), 278-282 (2004). HPTLC on silica gel with chloroform - acetonitrile - toluene - acetate buffer (pH 6.0) 50:40:10:3. Quantitative determination by absorbance measurement at 266 nm. The method was validated in terms of linearity, accuracy, precision, and specificity. The limit of detection and the limit of quantification were found to be 50 ng/spot and 100 ng/spot respectively. A simple, precise, accurate and rapid HPTLC method has been developed and validated for the simultaneous determination of cefuroxime axetil and probenecid in combined dosage form.

IPC 56th 2004, Abstract No. GP-26. HPTLC of triphala, an ayurvedic formulation containing about 3.60 % of total phenolics. Separation of alcoholic triphala extracts on silica gel with n-hexane - ethyl acetate 2:1. Rf value of the main spot gallic acid was 0.04 in triphala and its formulation. The method was found to be very specific for gallic acid having a linearity range of 0.2 - 1.6 mg/mL. Several formulations analyzed by HPTLC contained 5.2 - 7.6 % of gallic acid. The reported method is suitable for estimation of gallic acid in raw material and formulations.

Indian Drugs 42 (10), 650-653 (2005). A simple rapid, precise and cost-effective HPTLC method has been developed for the determination of ursolic acid in Oscimum sanctum (Tulsi) leaves and its formulations (Tulsi ghan tablets and Tulsi capsules). HPTLC on silica gel with toluene - ethyl acetate - acetic acid 30:3:1. Detection with anisaldehyde in sulphuric acid reagent followed by heating in an oven at 110 °C. Quantitative determination by absorbance measurement at 580 nm. Linearity of the detector response was given in the range of 40 - 280 ng. LOD was 8 ng. The correlation coefficient obtained from linearity was 0.9985. The standard error was 26.511. The mean assay values of ursolic acid wa found to be 3.485 mg/g, 0.553 mg/g and 3.221 mg/g in tulsi ghan tablets, tulsi capsule and tulsi leaves respectively.

59th Indian Pharmaceutical congress C-324, 302, (2007). HPTLC of aqueous and alcoholic extracts of different plant parts (fruits, leaves, stem and root) of Solanum xanthocarpum and Solanum trilobatum, on silica get with toluene - ethyl acetate - diethyl amine 7:2:1. Detection at 254 nm and 366 nm, and by spraying with antimony tretrachloride and vanilin - sulphuric acid.

Bio. Chromatogr. 21(1), 94-100 (2008). The method for detection of tyrosinase inhibiting substances involves spraying of the TLC layer with tyrosinase and l-tyrosine solutions successively. Positive results are detected as white spots against a brownish-purple background. The method is suitable either as a quick screening method for tyrosinase inhibitor detection or as a guiding procedure for an isolation of tyrosinase inhibitors from mixtures or natural product extracts. The technique is sensitive enough for results in the presence of 6 ng/zone glabridin.

Drug Development and Industrial Pharmacy 35, 440-448 (2009). TLC of tacrolimus on silica gel with toluene - acetonitrile - glacial acetic acid 60:40:1. For quantitative evaluation the plate was derivatized with anisaldehyde sulfuric acid reagent and scanned at 675 nm. Using this method a compact spot was obtained at an hRf value of 40. The linearity range was 100-800 ng/band. The method is suitable for routine analysis of formulations.

J. Chromatogr. A 1218 (19), 2745-2753 (2011). HPTLC of sucralose in waste water on silica gel with isopropyl acetate – methanol – water 15:3:1. The developing time was 15 min. Detection with p-aminobenzoic acid reagent. Quantification by absorbance measurement at 400 nm. The limit of quantification was 100 ng/L at a recovery rate of 80 % and the extraction of a 0.5 L water sample. An interlaboratory trial in 2008 showed good agreement of the sucralose content determined in four water samples by HPTLC and other methods (HPLC–MS/MS or HPLC–TOF-MS). The good accuracy and high sample throughput capacity proved HPTLC as a well suited method for quantification of sucralose in various aqueous matrices.

CBS 110, 5-7 (2013). HPTLC of extracts of (1) Nauclea diderrichii, (2) Sarcocephalus pobeguinii, (3) Hua gabonii, (4) Morinda lucida, and (5) Momordica foetida on silica gel with A) toluene - ethyl acetate 19:1; B) chloroform - methnaol - water 35:15:2; C) ethyl acetate - acetic acid - formic acid - water 100:11:11:27, D) acetonitrilie - water - formic acid 15:4:1, and E) 1-butanol - acetic acid - water 7:1:2. For (1) mobile phases B and C were best suited, for (2) mobile phase B, for lipophilic compounds of the essential oil of (3) mobile phase A and for (4) and (5) mobile phase B. Bioautographic analysis using alpha- and beta-glucosidase enzym assays, acetylcholinesterase inhibition assay, and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical reagent for detecting radical scavenging activity.