Chinese J. Chromatogr. (Sepu) 23 (2), 217-218 (2005). TLC of the title compound on silica gel with chloroform - benzene 7:3. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 120 ºC for 10 min; 2) by spraying with 10 % phosphomolybdic acid in ethanol followed by heating at 120 ºC for 10 min, then exposing to ammonia vapours. Quantification by comparison of the separated zone size with the standard. Optimization of the mobile phase by investigation of the influence of the composition of the developing solvent on Rf values. Optimization of the visualization condition by investigating the relationship between the sample dosage and the visualization results.

59th Indian Pharmaceutical Congress C-66, 240, (2007). Evaluation of Arjunakhseerpak and Arjunaristha, two ayurvedic formulations used for cardiovascular system, which contain Terminalia arjuna as the main ingredient. Simultaneous HPTLC of arjungenin and arjunolic acid from Terminalia arjuna on silica gel with toluene - ethyl acetate - acetic acid 10:10:1. Detection by spraying with 10 % sulphuric acid followed by heating. Densitometric evaluation at 366 nm. The UV spectra of the sandards arjungenin and arjunolic acid was found to be similar to that of arjungenin and arjunolic acid in the formulation. The method was found suitable for standardization of arjuna formulations.

Acta Chromatographica 6, 1-4 (1996). TLC of TNT (trotyl) on silica gel with hexane-benzene 1:1. Detection by spraying with phenol red, bromophenol blue, thymol blue, and bromothymol blue, separately, followed by heating at 100 °C for 10 min. These solutions were prepared immediately before spraying of the plates. The hRf value was 50 (± 2). Linearity was between 2.5 and 10 µg/zone. The correlation coefficient was 0.931. LOD (in average, n = 6) was 1.0 µg/zone (phenol red), 1.0 µg/zone (bromophenol blue), 1.5 µg/zone (thymol blue) and 1.5 µg/zone (bromothymol blue).

J. Liq. Chromatogr. Relat. Technol. 31, 2657-2672 (2008). TLC and HPTLC of thiuram on silica gel with methanol or dichloromethane in a saturated horizontal chamber for 15 min. Detection by spraying with improved and non-improved iodine-azide, iodine, and copper(II) reagents and by evaluation under UV 254 nm. For derivatization the developed plates were sprayed with freshly prepared mixtures of sodium azide and starch solution adjusted to pH 6.0 (5 mL of 10 % aqueous sodium azide solution and 12.5 mL of 2 % aqueous starch solution were mixed and adjusted to pH 6.0 with 0.1 mol/L hydrochloric acid solution; the solution was diluted to 25 mL with water) and exposed to iodine vapor for 5 s. Quantitative analysis by scanning with an office scanner (PC scanner) and after detection with improved iodine-azide reagent densitometric measurement at 483 nm. The hRf value of thiuram was 29. LOD were 3 and 0.5 pmol per spot using a iodine-azide detection system in TLC and HPTLC, respectively. Linearity was in the range of 2-8 pmol per spot; the correlation coefficient r was 0.9981. Results obtained by use of a PC scanner were comparable to measurements using a TLC densitometer.

J. of Chromatogr. A 1218 (19), 2684-2691 (2011). Review on TLC/bioautography. Discussion of three versions of bioautography, i.e. contact, immersion and direct bioautography. The focus is put on direct bioautography and many applications are quoted, not only for testing various groups of compounds, but also for investigating biochemical processes and factors influencing bacterial growth. Various related methods can be included into direct bioautography, of which TLC-bioluminescence screening is the most promising one.

CBS 109, 2-4 (2012). Extraction of arabica coffee with 2 to 50 % robusta coffee by accelerated solvent extraction with tBME. A part was saponified with10 % ethanolic KOH solution for 2 h. HPTLC of coffee extracts and standards 16-O-methylcafestol (16-OMC) and 16-OMC esters on silica gel with toluene - ethyl acetate - acetic acid 93:7:1 for 16-OMC esters and with tBME - chloroform 1:1 for 16-OMC. Detection by spraying with vanillin sulfuric acid (1 g in 250 mL ethanol and 2 mL of sulfuric acid, prepared freshly) and heating for 1 min at 80 °C. Quantitative absorption measurement at 530 nm. The LOD was 163 ng/band. After saponification the LOD of free 16-OMC was 43 ng/band. Thus 2 % robusta can be detected in a coffee blend.

J. Planar Chromatogr. 28, 139-143 (2015). TLC of 16 polyaromatic hydrocarbons on RP-18 phase. In the first direction, the plate is developed twice with n-pentane at -20 °C, and in the second direction with acetonitrile - methanol - water 12:8:3:3. Both developments were carried out over a distance of 43 mm. Detection by chemiluminescence: a solution of 225 mg Bis(2,4,6-trichlorophenyl)oxalate dissolved in 45 mL of n-butyl acetate was prepared. 125 μL hydrogen peroxide 30 % were vigorously shaken with this solution for 20 min. The mixture is suitable for chemiluminescence measurements within a period of 48 h. The best results were observed when the plate was dipped in this solution for 1 s and dried until no light reflection could be seen on the surface. Then, the TLC plate was covered by a glass plate and measured for 1 min using a very light-sensitive CCD camera. The proposed chemiluminescence screening method is able to separate PAHs from PAH oxidation products and detects benzo[a]pyrene and perylene with a LOD of 95 pg/band for benzo[a]pyrene and 48 pg/band for perylene. Although these compounds were separated from all other PAHs in the standard, a separation of both compounds was not possible from one another.

CBS 116, 11-12 (2016). HPTLC of lactose in milk, yoghurt, and instant sauce on silica gel with acetonitrile – water 3:1 (with 0.1 % trifluoroacetic acid) to the migration distance of 5 cm. Detection by spraying with aniline-diphenylamine-phosphoric acid reagent (2 g diphenylamine and 2 mL aniline in 80 mL methanol, 10 mL of o-phosphoric aicd 85 %, filled up to 100 mL) and heating for 10 min at 120 °C. Evaluation under white light. Elution of zones from the underivatized plate into ESI-MS with acetonitrile – water 19:1 (with 0.1 % formic acid) and detection in positive ionization mode. The method is suitable for semiquantitative determination of lactose by image evaluation and allows for confirmation of lactose-free products as such.