59th Indian Pharmaceutical congress F-11, 392, (2007). HPTLC oleanloic acid from seeds of Achyranthes aspera on silica gel with n-hexane - ethyl acatete - acetic acid 30:201. Detection by spraying with anisaldehyde - sulphuric acid reagent. Densitometry at 530 nm for quantification of oleanolic acid. Linearity was between 200 and 1200 ng/zone. The plant tree was found to contain 0.34 % oleanloic acid. The method can be used for routine quality control.

J. Agric. Food Chem. 56, 8050-8057 (2008). HPTLC of [2-14C]-cymoxanil in the cultures (medium and mycelium) and in the cell-free extracts of Botrytis cinerea on silica gel with hexane - ethyl acetate - acetic acid 70:30:1. The most polar products were separated on RP-18 (impregnated with a methanolic solution of tetrabutylammonium bromide 70 mM and dried at 80 °C for 5 min) with phosphate buffer (0.01 M, pH 6) – methanol 11:9. Detection by autoradiography. The hRf value of cymoxanil was 35 on silica gel. Different metabolites were detected using both stationary phases.

Encyclopedia of Chromatography Third Edition 1, 2319-2322 (2009). The author describes the techniques and instruments for the detection and quantification of radiolabeled substances in thin-layer radiochromatography (TLRC) or radio-TLC. In detail, film autoradiography, liquid scintillation counting (LSC), storage phosphor imaging and in situ radioactivity scanning are described. The instruments for these methods are highly automated providing significant advantages in radiopharmaceutical purity determination, metabolism studies and many other investigations.

J. Chromatogr. A 1218 (19), 2775-2784 (2011). A multi-enzyme inhibition assay (HPTLC-EI) based on rabbit-liver esterase (RLE) and cutinase following HPTLC allows detection of thiophosphate pesticides. Because choline esterase inhibition is more effective after conversion of thiophosphate thions into their corresponding oxons, a pre-oxidation step was added to the HPTLC-EI assay by using bromine vapor. Bromine was more effective than iodine or UV irradiation for oxidation. It increased the inhibitory strength of parathion, parathion-methyl, chlorpyrifos, chlorpyrifos-methyl, and malathion by 2 orders of magnitude. In contrast, bromine oxidation of organophosphate and carbamate insecticides resulted in a slight reduction in their inhibition factors, due to partial bromination and degradation of the parent compounds. Bromine oxidation increased the inhibition factors for demeton-S-methyl and propoxur. The HPTLC-EI system was applied to the analysis of apple juice and water samples spiked with paraoxon (0.001 mg/L), parathion (0.05 mg/L), and chlorpyrifos (0.5 mg/L) and the mean recoveries were 95-106 % and 91- 102% for RLE and cutinase, respectively.

CBS 108, 4-5 (2012). HPTLC of human insulin recombinant and porcine and bovine pancreas insulin on ProteoChrom silica gel with 2-butanol - pyridine - 25 % ammonia - water 39:34:10:26 to 50 mm migration distance. Detection under UV 366 nm after spraying with 0.02 % fluorescamine in acetone. Elution with the TLC-MS Interface and MS analysis in ESI positive mode, the eluent was acetonitrile - water 1:1. The hRf value of human, porcine and bovine insulin was 50. All mass spectra could be clearly assigned to the different insulin samples.

Journal of Ethnopharmacology 163, 192-202 (2015). TLC of the aqueous extract (crude vs. after tannin removal on a polyamide column) of the kino (red hydrosoluble exudate) of Pterocarpus erinaceus on silica gel with s-butanol – water – acetic acid 14:5:5. Detection with vanillin-hydrochloric acid reagent revealed catechic tannins and related polyphenols as dark pink zones. These compounds showed hRfs between 0 and 40, and above 60 in the case of the crude extract, whereas they almost did not appear in the tannin-free fraction. The extracts were also submitted to HPLC-HRMS, allowing the identification of epicatechin monomer in both extracts and of oligomers in the crude extract only.

CBS 113, 13-15 (2014). HPTLC of glycosylceramide Glc-d18:2 h16:0 from wheat germ and standards squalene, cholesteryl oleate, glyceryl trioleate, linoleic acid, ß-sitosterol, and ß-sitosterol glucoside on silica gel in the AMD 2 with a 18-step gradient modified from Opitz et al. (Chromatographia 73 (2011) 559), methanol replaced ethanol, and the mobile phase composition was changed slightly (pre-conditioning with 4 M acetic acid before each step, drying time 1.5 min, development duration 3 h and solvent consumption 200 mL). Detection by dipping in copper sulfate phosphoric acid reagent for 20 s and heating at 130 °C for 15 min revealed grey-brown bands. Densitometry evaluation by absorbance measurement at 546 nm. For Glc-d18:2 h16:0, regression analysis showed a polynomial relationship with coefficients of determination (R2) from 0.995 to 0.999 (n=3, 50 - 1000 ng/band). LOD (S/N 3) and LOQ (S/N 10) of Glc-d18:2_x000D_ h16:0 were 10 ng/band and 50 ng/band, respectively (n = 6).

Planta Medica 83(01/02), 104-110 (2017). TLC on silica gel 1) to monitor the column fractionation of the chloroform partition of a methanolic maceration of Caesalpinia pulcherrima roots (eluent not given); and for preparative isolation of 2) 8,9,11,14-didehydrovouacapen-5α-ol from a subfraction of the same (with n-hexane – dichloromethane – acetonitrile 50:50:1); 3) pulcherrimin E from another subfraction (with chloroform – methanol 49:1, followed by preparative HPLC); and 4) 6β-cinnamoyl-7β-acetoxyvouacapen-5α-ol and pulcherrimin D, obtained by acetylation (in pyridine and acetic anhydride) from cinnamoyl-7β-hydroxyvouacapen-5α-ol and pulcherrimin A, respectively (eluent not given). Detection by spraying with cerium sulfate and by exposure to iodine vapor._x000D_
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