J. Chromatogr. A 1059 (1-2), 171-174 (2004). The iodine-azide reaction was employed to TLC detection of sulphur-containing derivatives of protein and some non-protein amino acids. The derivatization reaction with phenyl isothiocyanate (PITC) took place directly on the plate before the developing step. Subsequently, the plates were sprayed with a mixture of sodium azide and starch solution in NP-TLC and in the case of RP-TLC sodium azide solution with starch incorporated into mobile phase and then exposed to iodine vapour. The spots became visible as white spots on violet-grey background. The obtained detection limits of PTC-derivatives have been compared with other visualizing techniques commonly used in TLC practice (UV254 and iodine vapour). The iodine-azide system has been proved to be the most favourable and enabled to detect quantities per spot in the range of 1-60 pmol (HPTLC) and 3–100 pmol (TLC).

J. AOAC Int. 90, 857-863 (2007). TLC of azadirachtin on silica gel with dichloromethane - ethanol 20:1. Detection by spraying with acidified vanillin reagent (3 g vanillin in 10 mL ethanol with 1.5 mL concentrated sulfuric acid) followed by heating at 110 °C for 3 min. Quantitation by densitometry.

J. Chromatogr. A 1164 (1-2), 298-305 (2007). TLC using staining reagents is fast, versatile and sometimes the only viable method method for analyzing organic compounds without chromophores. Investigation of quantitative TLC using staining reagents in combination with modern image analysis software showed that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. Illustration of the range of detection and the errors for the different parts of the process, which are largely due to the staining process but can be diminished by measuring ratios of compounds.

CBS 99, 9-11 (2007). HPTLC of petrochemical samples on silica gel pre- or post-chromatographically impregnated by dipping in methanolic berberine (60 mg/L) or coralyne (6 or 12 mg/L) solutions. Development in horizontal developing chamber with dichloromethane (saturated hydrocarbons), n-hexane (heavy gas oil), or petroleum ether - diethyl ether - acetic acid 80:20:1 (cholesterol). Quantitative determination by fluorescence measurement of berberine at 365/>450 nm and coralyne at 410/>450 nm. Linearity for alkenes was between 50 and 1500 ng and for naphtenes between 600 and 2400 ng.

Abstract No. 9162, IHCB (2009). HPTLC of glycyrrhizin in methanolic (70 %) extracts of Glycyrrhiza glabra on silica gel with chloroform - methanol - water 130:72:15. Quantitative determination by absorbance measurement at 254 nm and at 420 nm after spraying with anisaldehyde reagent. The method was linear in the range of 0.8-3.8 µg/spot. The limit of detection and quantification was 0.16 and 0.52 µg/spot, respectively. Glycyrrhizin was used as bioactive marker for quality control.

Analytical Chemistry - An Indian Journal 8(1), 77-81 (2009). An HPTLC method is reported for estimation of lupeol and beta-sitosterol in the whole plant powder of Asteracantha longifolia (Acanthaceae). The proposed method was also employed for estimation of beta-sitosterol in herbal formulation containing Asteracantha longifolia. Methanolic extracts of the sample were used for chromatography. HPTLC on silica gel with toluene - ethyl acetate - methanol 75:15:7. Derivatization with Liebermann-Burchard reagent. Densitometric quantification at 366 nm. The concentration of lupeol and beta-sitosterol was 0.162 mg/g and 0.045 mg/g, respectively in the whole plant, while the concentration of beta-sitosterol was 0.039-0.048 mg/g in the formulation. The method was suitable for the quality control of the herbal formulation.

J. AOAC Int. 93, 787-791 (2010). HPTLC of omeprazole on silica gel with chloroform - methanol 9:1 in a twin-trough chamber after saturation for 20 min. Quantitative determination by absorbance measurement at 302 nm. The hRf value of omeprazole was 39. Linearity was between 50 and 3000 ng/band. The intra-day and inter-day precision was 0.4-0.5 and 0.8-0.9 % (n = 2). The recovery was 98.4-99.1 %. LOD and LOQ were 8 and 24 ng/zone, respectively.

J Vet Sci Med Diagn 2(3), 1-3 (2013). HPTLC of neutral lipids on silica gel (HLF plates with 19 scored channels of 9 mm width) prewashed with dichloromethane - methanol 1:1 and heated for 30 min at 120 °C, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 with chamber saturation. Detection of dark zones on a yellow background after spraying with 5 % ethanolic phosphomolybdic acid reagent. Quantification of triacylglycerols by absorption measurement at 610 nm with polynomial regression via peak area.