IPC 56th 2004, Abstract No. GP-17. HPTLC of mirtazapine in tablet dosage form on silica gel with chloroform - methanol 1:9. The Rf value was 0.50 - 0.52, the linearity range was 0.3 - 1.5 mg/spot. Quantitative determination by scanning at 295 nm. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

The Pharma Review, Dec, 106-108 (2005). HPTLC of hexane, petroleum ether, chloroform, and methanol extracts of Arnebia nobilis (Ratanjot) roots on silica gel with n-hexane - methanol 9:1 and toluene - chloroform - methanol 14:5:1. Detection by spraying with anisaldehyde sulphuric acid reagent and densitometric fingerprint analysis by absorbance measurement at 366 nm. HPTLC fingerprinting profile provided the most reliable method for correct identification of the root.

59th Indian Pharmaceutical congress C-305, 297, (2007). Phytoconstituents of mature and immature tubers of Ipomoea mauritiana (methanolic and aqueous extracts) have been studied. HPTLC on silica gel with chloroform - methanol - formic acid 6:3:1. Detection by spraying with vanilin - sulphuric acid reagent. Densitometric evaluation at 365 nm. Mature tubers were found to contain higher concentration of phytoconstiuents than immature tubers.

Acta Chromatographica 9, 79-86 (1999). HPTLC (TLC) of glucose, maltose, sucrose and trehalose on silica gel with acetonitrile - water 17:3 and ethyl acetate – acetic acid – methanol – water 12:3:3:2 for three times with chamber saturation; on amino plates with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 and on cellulose plates with ethyl acetate – pyridine – water 2:1:2, both in a non-equilibrated chamber; on RP-18 with tetrahydrofuran – water 44:6 in a pre-equilibrated chamber. All plates were prewashed, e.g. by chromatography with dichloromethane - methanol 1:1. Detection by 4-aminobenzoic acid reagent, 1-naphthol–sulfuric acid reagent or aniline–DPA reagent, each followed by heating at 110 °C for 10 min. The combined results from comparison of hRf values for two different mobile phases on silica gel, on cellulose, amino phase and C18-bonded layers with diverse separation mechanisms, spiking experiments on silica gel, detection with selective reagents, and GC–MS analysis definitely proved the presence of maltose and glucose and the absence of trehalose in digestive gland–gonad complex and hemolymph samples from B. glabrata. Earlier papers reporting the presence of trehalose were undoubtedly in error.

J. Pharm. Biomed. Anal. 47, 841-846 (2008). HPTLC of iridoid glycosides in the aerial part of Gambhari (Gmelina arborea) with iridoid gycoside 6-0-(2”, 3”- dibenzoyl)-o-L-rhamnopyranosylcatalpol as a chemical marker for the standardization of G. arborea plant extracts on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 240 nm and at 430 nm after derivatization with vanillin - sulfuric acid reagent. The linear working range was between 1000-5000 ng/spot with a good correlation coefficient of 0.994.

International Journal of ChemTech Research 2(2), 807-812 (2010). Chloroform and ethyl acetate extracts of leaves and bark of Ficus mollis (Moraceae) were subjected to TLC fingerprint profiling on silica gel with toluene - ethyl acetate - formic acid 16:2:1. Evaluation under UV 254 nm. Derivatization with vanillin-sulfuric acid reagent, followed by heating at 105 °C until colorization. In the bark 7 well-defined zones were observed, whereas in leaves 10 zones were observed. Heavy metal and mineral analysis was performed by atomic absorption spectroscopy.

J. Planar Chromatogr. 26, 26-30 (2013). Four new spray reagents for the detection of amino acids were introduced: (1) 0.25 % solution of benzoic acid in ethanol, (2) 0.1 % solution of p-fluorobenzoic acid in ethanol, (3) 0.1% solution of p-chlorobenzoic acid in ethanol and (4) 0.05 % solution of p-iodobenzoic acid in ethanol. Depending on the different amino acids tested the LOD on silica gel was in the range of 0.1-1.0 µg for all four reagents. For example for cysteine the LOD was 0.1 µg with reagents (1), (3), and (4), and 0.2 µg with reagent (2).

J. Planar Chromatogr. 26, 508-509 (2013). TLC of endosulfan on silica gel with n-hexane - acetone 4:1. Detection by spraying with sodium hydroxide followed by fuchsin (1) or malachite green (2) reagents (50 mg in 100 mL water), followed by exposure to bromine gas for 3-5 min. The hRf values of endosulfan detected by (1) and (2) were 50 and 80, respectively.