Ind. J. Pharm. Sci. 68 (6), 838-840 (2006). HPTLC of ofloxacin and ornidazole in tablet dosage form on silica gel with n-butanol - ethanol - ammonia 5:5:4. Quantitative determination by absorbance measurement at 295 nm. The method was found to be linear in the concentrate range of 1-5 ng/spot with recovery of 99.5-102.5 % for both compounds. The method was validated for linearity, accuracy, precision, repeatability, and specificity.

J. Chromatogr. A 1046 (2), 251-257 (2007). Fluorescence scanning densitometry of various analytes on HPTLC silica gel plates impregnated with a solution of coralyne cation, based on the increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. Compared to a procedure previously described for berberine cation, and Reichardt's dye probes, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g. long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior.

J. Liq. Chromatogr. Relat. Technol. 31, 1492-1510 (2008). TLC of stearic acid, stearyl alcohol, and methyl stearate on silica gel (prewashed with methanol) with methanol - chloroform 1:1 followed by drying for 24 h at room temperature. Six new derivatization reagents were evaluated: gentian violet, methylene violet, methylene blue, methyl green, malachite green, and Janus blue. Detection by dipping for 5 s, followed by drying for 24 h at room temperature. Quantitative determination by absorbance measurement. The results obtained indicate that all of the new derivatization reagents give better results than the universally applied Rhodamine B. The best reagents for quantitative determination of stearic acid are methylene blue and Janus blue, for stearyl alcohol malachite green and Janus blue, and for methyl stearate methylene blue, Janus blue, and malachite green.

Abstract No. 9966, IHCB (2009). HPTLC of root tuber extracts of Asparagus gonoclados Baker on silica gel with ethyl acetate - methanol - water 15:3:2. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 425 nm. Shatavarin IV was used as marker.

Phytochem. Anal. 22, 59-65 (2011). TLC and free radical scavenging fingerprints of nineteen Salvia species on silica gel with toluene - ethyl acetate - formic acid 60:40:1 for the less polar constituents and ethyl acetate - water - formic acid - acetic acid 100:26:11:11 for the medium and highly polar substances. After drying at room temperature for 15 min derivatization with vanillin sulfuric acid reagent (1 g vanillin with 20 % sulfuric acid in methanol) followed by heating for 5 min at 105 °C. Quantitative determination by absorbance measurement at 254 nm. Free radical scavenging properties were investigated by spraying the plate with DPPH radical reagent (0.2 %) in methanol and left at ambient temperature for 30 min. The strongest free radical scavenging activity was observed for rosmarinic acid, with an hRf of 70.

International Journal of PharmTech Research 2(4), 2438-2440 (2010). TLC of methanolic extracts of powdered dry fruits of Embelia ribes on silica gel with toluene - acetone - acetic acid 18:2:1. Detection with anisaldehyde-sulphuric acid reagent. The hRf values of the four major bands were 13, 27, 32, and 60.

Trends in Chromatography 8, 1-6 (2013). HPTLC of neutral lipids and a neutral lipid standard (consisting of 20 % each of cholesterol, oleic acid, triolein, methyl oleate, and cholesteryl oleate) on silica gel (HLF plates with 19 scored channels of 9 mm width) prewashed with dichloromethane - methanol 1:1 and heated for 30 min at 120 °C, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 with chamber saturation. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating at 120 °C for 5 min. Neutral lipids appeared as blue zones on a yellow background. The fractions of free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters were identified by comparison with the standard mixture. Quantification by absorption measurement at 610 nm via linear calibration (peak area).

PLoS ONE 10(10), e0140782 (2015). HPTLC of lipids, extracted from several cancerous cell lines by an adapted Bligh and Dyer’s method, on silica gel with chloroform – methanol 4:1. Detection under UV after spraying with thioflavine S (10 μg/mL in acetone 80 %), glycosylated lipids were hued in red-rose by spraying with orcinol 0.5 % in sulfuric acid 0.5 M followed by heating; some of those lipids were absent from the elisidepsin-resistant strains. These lipids were purified by scraping off the plate and by extracting with chloroform – methanol 2:1, then they were applied on a nitrocellulose membrane for an overlay assay for binding with an elisidepsin-biotin derivative (dot-blotting). One lipid was positive and was identified by NMR as probably a glucosylceramide.