J. of Chromatogr. A 1248, 169-177 (2012). Purified oligomers of hyalobiuronic acid are indispensable tools to elucidate the physiological and pathophysiological role of hyaluronan degradation by various hyaluronidase isoenzymes. Establishment and validation of a novel sensitive, convenient, rapid, and cost-effective HPTLC method for the qualitative and quantitative analysis of small saturated hyaluronan oligosaccharides consisting of 2–4 hyalobiuronic acid moieties. HPTLC on amino phase with 1-butanol - formic acid - water 3:5:2 or 3:4:1. Detection 1) by spraying with orcinol in various concentrations of sulfuric acid; 2) by dipping into the reagent of orcinol in 10 % sulfuric acid and Morgan–Elson reagent; 3) by illuminating with white light and UV 366 nm after heating. The simple reagent-free in situ derivatization of 3) resulted in a detection limit of 7–19 pmol/band and LOQ of 37–71 pmol/band depending on the analyzed saturated oligosaccharide. Identification of the analytes by TLC-ESI-MS. The validated HPTLC method, as an alternative to sequential techniques such as HPLC and CE, can easily be automated and is applicable to the analysis of multiple samples in parallel.

J. Planar Chromatogr. 26, 375-378 (2013). TLC of estrone, estradiol, and estriol on silica gel with chloroform - ethyl acetate - acetone 6:2:1. Detecion by dipping into (1) 10 % solution of phosphomolybdic acid (PMA) in methanol, followed by heating at 100 ºC for 10 min; (2) 0.2 % ceric ammonium sulfate in phosphoric acid, followed by heating at 110 ºC for 10 min; (3) 0.2 g manganese(II) chloride in 30 mL water, 30 mL methanol and 2 mL concentrated sulfuric acid, followed by heating at 100-120 ºC for 10-15 min; and (4) 1 g of vanillin in 25 mL of ethanol, 25 mL of distilled water, and 35 mL of ortho-phosphoric acid (85 %), followed by heating at 120-160 ºC for 5-15 min. The lowest detection limit for estrone (75 ng/zone) was achieved using (1) and (3), whereas for estradiol and estriol (both 4.7 ng per zone) by using (2).

J. Liq. Chromatogr. Relat. Technol. 39, 303-307 (2016). HPTLC fingerprinting of 27 white wines of three sorts on silica gel with ethyl acetate – formic acid – acetic acid – water 10:1:1:2 and on RP-18 with methanol – water – formic acid 50:50:1. Detection by heating at 100 °C for 3 min, followed by dipping into Natural Product reagent (1 g of diphenylborinic acid aminoethylester dissolved in 200 mL ethyl acetate) and after drying by dipping into PEG reagent (10 g of polyethylene glycol 400 dissolved in 200 mL dichloromethane). Qualitative identification under UV 254 nm and UV 366 nm.

Planta Medica 82 (16), 1438-1445 (2016). TLC on silica gel with chloroform – methanol 19:1, followed by detection under UV 254 nm and spraying with 10 % sulfuric acid with heating. TLC was used for 1) the ethyl acetate fraction of a hydromethanolic extract of Eryngium triquetrum aerial parts, and 2) for the monitoring of its fractionation on a cyclodextrin column, and of the fractionation of a selected fraction on silica gel column. The (chloroformic) first subfraction of the last step was found to contain only the polyyne alcohol falcarinol (panaxynol, carotatoxin) at hRF 90.

Phytochem. Anal. 29, 5-15 (2018). TLC fingerprint of Ipomoea aquatica on silica gel with methanol – water from 1:1 to 9:1 (1) or methanol with 0.05 % trifluoroacetic acid – water with 0.05 % trifluoroacetic acid 1:1 (2). Detection by spraying with anisaldehyde sulfuric acid reagent. Detection under UV 254 nm, 366 nm, and for (2), white light (yellow bands at hRF 19 and 25). The post chromatographic visualization possibilities indicate the potential of TLC in qualitative fingerprint analysis.

Planta Med. 83(09), 797-804 (2017). For the analysis of their sugar moieties, five new triterpene saponins (ilexpusons B, D, E, F, G), isolated from a methanolic extract of Ilex pubescens roots, were hydrolyzed at 90 °C in water containing HCl and dioxane. TLC of the dried aqueous phases on silica gel with chloroform – methanol – water 8:5:1. Detection by spraying with aniline – diphenylamine reagent (2 mL aniline, 2 g diphenylamine, 10 mL phosphoric acid 85%, in 100 mL acetone) and heating for 2-5 min at 85 °C. By comparison with standards, the sugar moiety was identified (before confirmation by GC) as glucose (hRF 49) in ilexpuson B, as xylose (hRF 81) in ilexpusons D and E, and as glucuronic acid (hRF 44) in ilexpusons F and G.

J. Planar Chromatogr. 3, 435-436 (1990). Review of TLC separation of phenols with 21 references. Reported is the separation of 11 phenols on silica resp. a mixture of silica and 10 or 15% sodium phosphotungstate in the presence of 3% agar-agar - polyvinyl alcohol 1:2 with benzene - diethylamine 4:1. Visualization by spraying with a fresh aqueous solution of 1.25% Kodak CD-3 - 2.5% Na2CO3 1:1. Detection limit 10-2 µg/spot; the method can also be applied to quantitative in situ analysis.

J. Planar Chromatogr. 3, 271-272 (1990). TLC of mercury-metal mixtures (as cations) on stannic silicate as ion-exchange layer with 1 M NH4Br. Detection after drying by spraying with copper solution (5 % CuSO4 · 5 H2O) and, after drying again, with a solution containing 5 g KI and 10 g Na2SO3 in 100 mL water. Quantification by densitometry (absorbance at 500 nm).