Planta Medica 82 (16), 1395-1402 (2016). The hexane – ethyl acetate subfraction of the ethyl acetate fraction of an ethanolic Tabebuia roseoalba leaf percolation extract was subfractioned on a silica gel column with 42500 mL of a gradient of hexane, ethyl acetate, and methanol. For monitoring, TLC on silica gel with hexane – ethyl acetate 1:1, detection with anisaldehyde sulfuric acid reagent. Two fractions that gave only one visible zone on TLC, were identified by NMR as mixtures of stigmasterol and sitosterol, and of alpha- and beta-amyrins, respectively.

Food. Res. Int. 102, 282-290 (2017). HPTLC of fructooligosaccharides in pure orange juice on silica gel with n-butanol – 2-propanol – water 10:5:4. Detection by spraying with a mixture of phosphoric acid (6.8 mL), urea (3 g) and ethanol (8 mL) in 100 mL of 1-butanol – water 8:1, followed by heating at 120 °C for 10 min. Quantitative determination by absorbance measurement at 450 nm.

Planta Medica 83(14/15), 1149-1158 (2017). The cyclodextrane fractionation of the methanolic percolate of Salix reticulata aerial parts was analyzed on silica gel with ethyl acetate – methanol – water 100:13:10. Detection by derivatization with vanillin-sulfuric acid reagent. Luteolin and apigenin glycosides, catechin, procyanidins and phenolic glucosides (picein, triandrin, and salicortin) were isolated from these fractions through preparative HPLC._x000D_

J. Planar Chromatogr. 3, 228-231 (1990). HPTLC separation of neutral lipids and phospholipids on silica with hexane - ether 6:4 (for neutral lipids) and chloroform - methanol - triethylamine - water 30:35:35:8 for phospholipids. detection by immersion for 1 min in a modified molybdate reagent. Quantification by densitometry at 595 nm.

J. Chromatogr. 564, 272-277 (1991). TLC on silica with petrol ether - ether (peroxide free) – acetic acid 90:30:1. Detection by spraying with chloroform – methanol 1:1 containing 232 x 10-6 M 2,5-bis-2-(5-tert.-butylbenzoxazolyl)thiophene, and inspecting under UV 365 nm. Quantification by GC.

Biochemical Systematics and Ecology 19, 685-695 (1991). TLC of quinolizidine alkaloids on silica with chloroform – cyclohexane – butylamine 5:4:1. TLC on aluminium oxide with 1,5 % methanol in chloroform. After heating at 100 °C for 3 min visualization under UV 254 and 365 nm and by spraying with iodoplatinate reagent.

Amer. Lab. 24, 50T (1992). Discussion of parameters for visualization of hard layer TLC and HPTLC plates to change the sensitivity, color , limit of detection, or reaction speed to the advantage of the detection required.

J. Planar Chromatogr. 5, 465-467 (1992). TLC of 8 azaarenes on cellulose with DMF - water mixtures 65:35, 50:50, 35:65, 20:80, 10:90. After drying visualization under UV 254 nm and exposure to trifluoroacetic acid vapor followed by visualization under UV.