J. Sci. Res. 1(1), 121-127 (2009). An HPTLC method is described for qualitative and quantitative estimation of sinapic acid in Lepidium sativum (Brassicaceae). Methanolic extracts of the plant material were subjected to chromatographic separation on HPTLC silica gel with n-butanol - acetic acid - water 4:1:5. Derivatization with anisaldehyde sulfuric acid reagent, followed by heating at 110 °C for 10 min. Denstiometric quantification at 326 nm. Identification was confirmed by comparison of hRf-values of sample and standard.

International Journal of PharmTech Research 2(2), 1427-1430 (2010). Ashokarista formulations contain ashoka (Saraca indica) as the main ingredient. Its markers are catechin, (+)catechole, and (-)epicatechin. TLC of extracts and (+)catechin on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:0.1. Quantitative determination by densitometry in absorbance mode at 278 nm. For identification of the stem-bark of Saraca indica the fingerprint is evaluated after detection with anisaldehyde-sulphuric acid. The hRf value of (+)catechin was 54.

International Journal of Medical Toxicology & Legal Medicine 15 (1&2), 61-63 (2012). HPTLC for five benzodiazepines (alprazolam, clonazepam, diazepam, lorazepam and nitrazepam) on silica gel G with chloroform - acetic acid 9:1 in a presaturated TLC chamber. Detection by evaporisation with chlorine gas for 5 min, and after complete removal of chlorine, by spraying with o-tolidine reagent. Stabilisation of blue colored zones for a day by spraying the plate with 1 % phosphomolybdic acid. LOD for alprazolam, clonazepam, diazepan, lorazepam and nitrazepam was found to be 0.1, 0.5, 0.5, 1 and 0.5 µg/zone, respectively.

American Journal of Analytical Chemistry. 3, 669-674 (2012). TLC of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with 0.01 % 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline in ethanol (reagent 1), followed by drying, then spraying with 0.25 % ninhydrin in acetone (reagent 2), and heating at 110 °C for 10 min. The reagent 1 produced various distinguishable colors and LODs between 5 and 200 ng/zone.

CBS 113, 9 (2014). HPTLC of caffeine in energy drinks (applied neatly without sample preparation) on silica gel MS-grade with 2-propanol – n-heptane – water 7:3:1. Detection under UV 254 nm and by dipping into anisaldehyde reagent. Quantitative evaluation by absorbance measurement at 273 nm. The hRf of caffeine was 55. For confirmation of results, zones were eluted with the TLC-MS Interface (acetonitrile – water 19:1 with 0.1 % formic acid, flow rate 0.1 mL/min) and directly transferred to a mass spectrometer and measured in ESI(+) full scan mode (m/z 100 – 500).

Planta Medica 82(18), 1568-1575 (2016). The flash chromatography fractionation of an enriched hydro-methanolic extract of Maesa argentea leaves was monitored through TLC on silica gel with n-butanol – acetic acid – water 4:1:5. Saponins (including four new antiparasitic triterpenoids, maesargentosides I-IV) were detected with anisaldehyde sulfuric acid reagent.

J. Chromatogr. A 1560, 97-103 (2018). Proposal of a simple and convenient on-spot derivatization approach for the modification of hydroxyl-containing compounds for their analysis by TLC/MALDI. Post-chromatographic acylation of separated analytes with 3-bromopropionyl chloride with simultaneous quaternization of pyridine, resulted in derivatives with permanent positive charges, which, in contrast to the initial alcohols not ionizable under TLC/MALDI conditions, reveal intense peaks of their cationic moieties in MALDI mass spectra recorded directly from TLC plates. Demonstration of the method by applying to a series of mammalian and plant sterols, phenols and terpene alcohols.

J. Planar Chromatogr. 3, 307-310 (1990). The liquid crystalline method for the visualization of thin layer chromatograms involves mapping the chromatogram by transferring the organic substances from the plate to a liquid crystal layer. Because the properties of the substrate have no significant influence on the transfer of the substances to be mapped it should be possible to use liquid crystal detectors to detect substances adsorbed on any substrate, can be used to visualize spots mapped from carbon layers. Method demonstrated with toxic substances used in warfare (o-ethyl-s-diisopropylaminoethyl ester of methanephosphoric acid) pesticides.