Biochemical Systematics and Ecology 19, 665-667 (1991). TLC of hydrolysed sugars on cellulose with pyridine – ethyl acetate – acetic acid – water 36:36:7:21, followed by staining with aniline diphenylamine phosphate. TLC of C-glycosyl flavones with 15% acetic acid on cellulose. Visualization by spraying with Naturstoff A reagent.

J. Chinese Herb Med. (Zhongcaoyao) 23, 23-25 (1992). TLC of peiminine, peimine hupehinine, hupeheinoside and ebieusine on silica with benzene – ethyl acetate – diethylamine 6:4:1. Detection by spraying with potassium iodobismuthate reagent. Quantification by densitometry (absorbance) at 410, 480 and 490 nm.

J. Planar Chromatogr. 4, 397-401 (1991). TLC of triglycerides (i.a. trilinolein, triolein, dipalmitoolein, palmitostearoolein) on silica impregnated with 0.5% methanolic silver nitrate solution or on RP-2 silica. Eluent for argentation TLC was petrol ether - acetone 20:9. After development the plates were dried for at 110°C, then treated consecutively with bromine vapor and sulfuryl chloride vapor, followed by charring at 180-200°C. Densitometry.

J. Planar Chromatogr. 5, 469-471 (1992). TLC separation of metal dithizonates, and diethyldithiocarbamates (lead, cadmium, zinc, copper, nickel, manganese, cobalt) and chlorides or nitrates on silica with toluene - chloroform 50:1 resp. with benzene - methyl isopropylketone 50:1; nitrates or chlorides on RP-18 silica with methanol - water - acetic acid 50:30:4. Visualization of metal diethyldithiocarbamates with 5% aqueous copper sulfate solution, of metal ions on RP-18 plates by treatment with a solution of 0.25 % PAN (1-(2-pyridylazo)-2-naphthol) in methanol followed by exposure to NH3.

International Laboratory News, February, 8A-8B (1994). HPTLC of clenbuterol in various matrices on silica in horizontal developing chamber from both sides with ethyl acetate - methanol - acetic acid 8:1:1 with chamber saturation. Derivatization by spraying with Bratton-Marshall reagent (first 1% sodium nitrite in 1 N HCl, then 0.4% N-(1-naphthyl)-ethylenediamine dihydrochloride in methanol). Quantification by densitometry (absorbance) at 525 nm. Detection limit 2.5 ng; determination limit 10 ng. Further betablockers can be detected with this method, like atenolol at hRf 8, salbutamol at 14, propranolol at 25, clenbuterol at 40 and labetalol at 44. Sample preparation includes deproteinization, defattening and final extraction from an alkaline aqueous phase. Very fast method - chromatography of 18 resp. 36 samples within 10 min.

Br. using gymnestrogenin as reference. Indian J. Pharm. Sci. 66 (2), 242-244 (2004). HPTLC of gymnestrogenin in Gymnema sylvestre on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 293 nm. Linearity was in the range of 4-10 µg. A gymnestrogenin content of 1.11 % was found in the test sample. Average percentage recovery was 99.1 ±0.27. The proposed method is precise and sensitive and can be used for detection, monitoring, and quantification of gymnestrogenin in Gymnema sylvestre.

56th IPC 2004, Abstract No. GP-36. HPTLC for the standardization of gallic acid in alcoholic extracts of Aphanamixis polystachya (Meliceae) on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. Rf value of gallic acid was 0.45, linearity was 15 - 75 mg/mL. Formulations were found to contain 9.56 % of gallic acid. Gallic acid is the main phenolic compound and can be used for standardization of the crude drug.

J. Liq. Chrom. & Rel. Technol. 26, 3453-3462 (2003). HPTLC of caffeine and acetaminophen on silica gel in a saturated twin-trough chamber with ethyl acetate - glacial acetic acid 19:1. Quantification at 254 nm. Diphenhydramine, pseudoephedrine, and acetaminophen were well separated from the caffeine zone. Precision (relative standard deviation) was 1.19 %; limit of detection was 0.2 µg for caffeine and 0.08 µg for acetaminophen; precision of duplicate samples (RSD) ranged from 0.95 to 7.56 %.