J. Planar Chromatogr. 33, 579-585 (2020). HPTLC of aloe-emodin (1), rhein (2) and emodin (3) on silica gel with petroleum ether - ethyl acetate - formic acid 31:10:2. Quantitative determination by absorbance measurement at 440 nm. The hRF values for (1) to (3) were 47, 36 and 25, respectively. Linearity was between 90 and 426 ng/zone for (1), 64 and 304 ng/zone for (2) and 80 and 380 ng/zone for (3). Intermediate precision was below 2 % (n=6). Average recovery was 98.6 % for (1), 99.7 % for (2) and 100.0 % for (3).

Planta Med. 84(18), 1348-1354 (2018). A subfraction (obtained through liquid-liquid partition and column chromatography) of the ethanolic extract of whole Vernonia cinerea plants (Asteraceae, subf. Cichorioideae) was further fractioned by reverse-phase SPE (solid-phase extraction) followed by preparative TLC on silica gel layer (eluent not given). For verification, zones were detected by spraying with anisaldehyde solution with 10 % sulfuric acid, followed by heating at 100 °C. Further purification by reverse-phase HPLC allowed the isolation of 6 hirsutinolide-type sesquiterpenoids (all with a oxacyclonane forming an ether bridge), including vernolides A and B.

Planta Med. 84(18), 1340-1347 (2018). Preparative TLC on silica gel for the final purification of 15 phenylethyl-chromenone derivatives (including 3 with epoxide functions), 4 sesquiterpenoids (neopetasane, dehydrokaranone, dioxoselinene, ligudicin C) and a steroid (ergostatetraenone), all isolated through multi-step column chromatography from the ethyl-acetate fraction of a methanolic extract of Aquilaria sinensis resinous wood (Thymelaeaceae). For each compound, mobile phase, RF value and yield are given.

J. Sep. Sci. 43, 3885-3901 (2020). HPTLC of erianin and gigantol in the stems of Dendrobium hercoglossum on polyamide film with methyl benzene - ethyl formate - formic acid 6:3:1. Detection by spraying with 10 % ethanol solution of sulfuric acid, followed by heating at 105 ºC.

J. Liq. Chromatogr. Relat. Technol. 42, 249-257 (2019). HPTLC of methanolic extracts from the leaves of Paulownia tomentosa on silica gel with chloroform - ethyl acetate - methanol 20:3:2. HPTLC-direct bioautography by dipping into B. subtilis cell suspension, followed by incubation at 28 °C for 2 h. Then the bioautograms were dipped into an aqueous solution of the MTT vital dye (1 mg/mL (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), followed by incubation at 28 °C for 30 min. Further analysis by using a HPLC-DAD-MS system allowed the identification of apigenin and p-coumaric acid as highly abundant antibacterial components.

J. Planar Chromatogr. 32, 223-229 (2019). HPTLC of syringaresinol in bark pieces of Gnidia glauca on silica gel with ethyl acetate - methanol 4:1. Quantitative determination by absorbance measurement at 273 nm. The marker compound was identified by high-resolution mass spectrometry.

J. Planar Chromatogr. 32, 231-235 (2019). HPTLC of mangiferin in Canscora perfoliata on silica gel with ethyl acetate - methanol - formic acid - acetic acid - water 10:1:1:1:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value of mangiferin was 53. Linearity was between 0.1 and 0.5 µg/zone. The intra-day and interday precisions were below 1 % (n=7). The LOD and LOQ were 30 and 50 ng/zone, respectively. Recovery rate was 97.5 %.

J. Planar Chromatogr. 32, 55-57 (2019). TLC of dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, diisobutyl phthalate, diallyl phthalate, and bis(2-ethylhexyl) phthalate on silica gel with petroleum ether - ethyl acetate - phosphoric acid 9:1:1. Detection by spraying with 0.1 % 2,6-dichlorophenolindophenol with the instantaneous appearance of pink-colored zones.