J. Planar Chromatogr. 29, 361-365 (2016). Analysis of phenols in bio-oil from agricultural wastes obtained during fractionation. Column chromatography (CC) on silica gel in a glass column (i.d. 1 cm, 50 cm length) filled with n-hexane. The concentrated organic phase was mixed with silica gel 1:1, loaded onto the column and successively eluted with dichloromethane – acetone 20:1, ethyl acetate, and methanol. 24 eluate fractions were obtained during the fractionation using CC, 12 with dichloromethane – acetone mixture, 5 with ethyl acetate, and 7 with methanol. Phenolic compounds were present only in the dichloromethane – acetone mixture fraction which was therefore selected as the proper eluent to obtain all the phenolic fractions dissolved. To monitor the fractionation, TLC of the eluates on silica gel in saturated chambers (mobile phase not specified) to a distance of 4 cm. Qualitative identification under UV 254 nm and by exposure to iodine vapor. The hRf values of the standard solutions of phenol, cresol and guaiacolin dichlorlomethane were 66, 68 and 78, respectively. Eluate fractions were further analyzed by GC–FID and GC–MS.

J. Planar Chromatogr. 1, 220-226 (1988). Description of the construction and the characteristics of an interface for coupling CLC and TLC. Investigation of proper eluent for deposition. Discussion of the shape of the deposited spots and chromatographic features and the repeatability of the deposition process.

Anal. Biochem. 188, 181-186 (1990). TLC of GPC fractions of NaBH4-labeled oligosaccharides on 3-aminopropyl-bonded silica with acetonitrile - 10 mM triethylamine acetate 3:2. Detection and mapping according to size, anionic charge, and sugar composition. Determination of the distribution of oligosaccharides on bovine submaxillary mucin and rat gastric mucin.

Chromatographia 35, 627-630 (1993). TLC separation of free fatty acids into two fractions, a more mobile group of saturated and monounsaturated fatty acids and a less mobile group containing polyunsaturated fatty acids, on silica with hexane - acetic acid - water 50:2:1. Detection by exposure to iodine vapor, and under UV 254 nm. Determination of polyunsaturated fatty acids by HPLC.

J. Chromatogr. A 803, 235-239 (1998). Modeling of ion-pair HPLC separations by using continuous OPLC. Study of the distribution of several cationic ion-pairing reagents on the layer with mobile phases containing different quaternary ammonium salts, which volumes varied between 4.5 to 67.5 cm3 equivalent to a 1 to 15 bed volume of the given layer. Measurement of the concentration of the reagents after chromatography in 10 samples of the stationary phase used.

Chromatographia 52 (3/4), 175-178 (2000). Investigation of methods for the separation of test mixtures of 10 flavonoids using isocratic RP-HPLC, gradient RP-HPLC, and a coupled isocratic RP-HPLC-NP-TLC system. TLC of the flavonoid fractions separated on RP column, by using 3-step gradient elution with methanol - ethyl acetate, giving a complete separation of the investigated compounds.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (10), 798-801 (2004). TLC on silica gel with 1) ethyl acetate - butanone - formic acid - water 10:7:1:1; 2) benzene - acetone 10:1; 3) petroleum ether - ethyl formate - glacial acetic acid 18:1:1. Detection 1) under UV 365 nm, 2) by spraying with 5 % solution of vanillin - acetic acid - perchloric acid and heating at 105 ºC, 3) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC. Identification by fingerprint techniques. Quantitation of icariine by HPLC.

J. Chromatogr. Sci. 54 (4), 639-646 (2016). Presentation of a low-cost method for digital image analysis of the separation results of colorless analytes on TLC plates. Quantitative TLC-digital image analysis was performed using a universal staining reagent (iodine vapor), an office scanner and a commonly available software (Microsoft Paint) for analysis of red, green and blue colors (RGB values). Urinary creatinine is used as a model analyte to represent a sample in complicated biological matrices. TLC on silica gel with butanol – ammonia solution – water 4:1:5 up to 6 cm. Detection by exposure to iodine vapors for 30–60 min. The Green value offered the best results in the linear working range of 0.08 - 0.93 mg/mL. The precision (%RSD) was 2.0 % (n=10), and the LOD 0.24 µg per zone. Comparison of the urinary creatinine concentrations determined by TLC-digital image analysis using the green value calibration graph with results obtained by HPLC showed that both are well consistent.