J. Planar Chromatogr. 22, 43-47 (2009). HPTLC of alpha-solanine and alpha-chaconine on silica gel with dichloromethane - methanol - 2.5 % ammonia 175: 75:11 in a horizontal chamber saturated for 15 min. After drying and heating at 90 °C for 25 min detection by dipping twice in modified Carr-Price reagent (antimony(III) chloride in acetic acid - dichloromethane), followed by heating at 110 °C for 3 min. Quantitative determination by absorbance measurement at 560 nm. Linearity was between 30 and 700 ng. The limit of detection was 5-20 ng/zone depending on the sample matrix, the limit of quantification was 30 ng/zone. The ratio of alpha-chaconine and alpha-solanine was between 4:1 to 2:1 for all analyzed samples.

Phytochem. Anal. 25, 29-35 (2014). HPTLC of hyoscyamine (1), scopolamine (2) and their biosynthetic precursors anisodamine (3), cuscohygrine (4) and litorine (5) in selected Solanaceae plants on silica gel with two-stage multi-development using chloroform - methanol - acetone - 15:3:2 + 1 drop ammonia. Quantitative determination by absorbance measurement at 190 nm for (3) and at 520 nm for (1), (2), (4) and (5) after spraying and derivatising with Dragendorff’s reagent following a Munier and Macheboeuf modi?cation (Baerheim-Svendsen and Verpoorte, 1983). The hRf values for compounds (1) to (5) were 41, 78, 24, 30 and 49, respectively. Linearity was in the range of 1000-3000 ng/zone for (3) and 500-3000 ng/zone for (1), (2), (4) and (5). LOD and LOQ were 127 and 425 ng/zone for (1), 100 and 334 ng/zone for (2), 11 and 38 ng/zone for (3), 104 and 413 ng/zone for (4), and 111 and 373 ng/zone for (5), respectively. Recoveries were in the range of 88-91 % for all the analytes. Intermediate intra- and inter-day precision was below 3 % (n=3).

Planta Medica 82,09/10, 910-918 (2016). After a fermentation process, the broths of psychrotolerant strains Vibrio splendidus T262 (isolated from the digestive tract of an Antarctic Ocean fish) and Arthrobacter psychrochitiniphilus T406 (found in penguin excrements in King George Island) were extracted with ethyl acetate and fractioned. To check the purification of new bisindole alkaloids from V. splendidus (turbomycines C-F) (1), and of A. psychr. (2-(indol-3-yl-methyl)indol-3-yl ethanol (2), TLC on silica gel (with water-resistant binders) with hexane – ethyl acetate 9:1 for (1) and dichloromethane – methanol 9:1 for (2). Evaluation before and after treatment with anisaldehyde reagent. Also preparative TLC on silica gel to isolate the following alkaloids from V. splendidus: monoindole: 4-(1H-indol-3-yl-sulfanyl)phenol (hRF 45 with hexane – ethyl acetate 17:3); bisindoles: 2,2-di-(3-indolyl)-3-indolone and 4-bis(1H-indol-3-yl)methyl]-cyclohexa-2,5-dienone (eluent not given); and trisindoles: trisindolal (hRF 58 with dichloromethane – methanol 9:1), metagenetriindole (hRF 45 with the same), and turbomycine A (not given).

Planta Med. 83, 912-920 (2017). Two subfractions of the ethanol extract of Aristolochia cordigera roots were mixed and submitted to preparative TLC on silica gel with hexanes (mixture of hexane isomers) – ethyl acetate 3:2 to isolate hyrtiosulawesine, aristolochic acids I and IV, sodium aristolochates I and IVa. The same method applied to a subfraction of the acetone extract of the roots revealed hyrtiosulawesine and 3,4-dihydro-hyrtiosulawesine.

Phytochemistry !21, 2365-2388 (1982). TLC of lupin alkaloids on silica with dichloromethane - methanol -28 % NH3 90:9:1, dichloromethane - methanol 4:1, ether - methanol -28 % NH3 70:30:1 and on aluminum oxide with benzene - methanol - acetone 34:3:3. Detection by UV and with Dragendorff reagent. Isolation of the alkaloids from plant extracts by PLC. Also HPLC of the isolated compounds.

Synthesis of 5-(per-O-acyl-beta-D-glycosyl)2-halogeno-methyl- and 2-azidomethyl-1,3,4-oxadiazoles. Acta Chimica 115, 319-325 (1984). TLC of S-(per-O-acyl-beta-D-glycosyl)-2-halogeno-methyl and 2-azidomethyl-1,3,4-oxadiazoles on silica with acetone - petrol ether 4:1. Detection by heating.

(Separation and determination of the metabolites of heptacaine hydrochloride by TLC and spectrophotometry.) Pharmazie 40, 583-584 (1985). Chromatography of rat liver extracts on silica with petrol ether - diethylamine 7:1; spectrophotometry after elution; identified metabolites: heptacaine-N-oxide, 2-heptoxyaniline, piperidino ethanol.

J.A.O.A.C. 67, 542-547 (1984). TLC of strychnine on silica with dichloromethane - methanol - water - formic acid - diethylamine 72.3:25:2.5:0.1:0.1. Detection by UV and spraying with acidic iodoplatinic reagent.