J. Sep. Sci. 32, 3239-3245 (2009). HPTLC of shikonin (1), acetylshikonin (2), and beta-acetoxyisovalerylshikonin (3) in four species of Arnebia on RP-18 with acetonitrile - methanol - 5 % formic acid 20:1:4. Quantitative determination by absorbance measurement at 520 nm. Linearity was in the range of 100-600 ng/zone for (1) and (2) and 100-1800 ng/zone for (3). The limits of detection for (1), (2) and (3) were 18, 15 and 12 ng/zone, respectively, while the limits of quantification were 60, 45 and 40 ng/zone, respectively.

Chinese J. Pharm. Anal. 28 (11), 1800-1803 (2008). TLC of adenosine and adenine in Lingzhi capsules on silica gel with chloroform – ethyl acetate – i-propanol – water 16:6:3:1. Detection under UV 254 nm. The method is simple, fast and accurate and can be used for the quality control of the medicine.

J. Ethnopharmacol. 124, 450-456 (2010). HPTLC fingerprinting of Eclipta alba on silica gel with chloroform - ethanol - water 35:10:2. Densitometric evaluation at 254 nm. Major compounds identified were coumestants and wedelolactone, with hair growth promoting activity.

Abstract No. C-47, 61st IPC (2009). HPTLC of piperine and vasicine in polyherbal cough formulations on silica gel with dioxane - toluene - ethyl acetate - methanol - 25 % ammonia 15:20:10:10:3. Quantitative determination by absorbance measurement at 304 nm. The method was validated for accuracy, precision, LOD, LOQ, linearity and specificity and was found to be linear in the range of 2-10 µg/band for both vasicine and piperine.

Rapid Commun. Mass Spectrom. 24, 1721-1729 (2010). Ambient proximal probe thermal desorption (TD) sampling of substances from a HPTLC plate and coupled with secondary ionization by atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI). The method does not require a specialized ionization source. Different anaytical parameters and performance metrics are reported and the method covers a wide range of analyte types including explosives, dyestuffs, herbicides and pharmaceuticals.

Eurasian J. Anal. Chem 5(1), 95-103 (2010). An HPTLC method is reported for quantification of glycyrrhetinic acid and apigenin in Glycyrrhiza glabra. Methanolic and acidic-methanolic extracts are subjected to chromatographic separation on silica gel with ethyl acetate - ethanol - water - 25 % ammonia 63:20:4:1. The method was linear in the range of 160-960 ng/band for glycyrrhetinic acid and 32-96 ng/band for apigenin. Glycyrrhiza glabra was found to contain 0.65 % of glycyrrhetinic acid and 0.0004 % of apigenin.

Eurasian J. Anal. Chem. 5(1), 25-27 (2010). An HPTLC method is reported for estimation of levodopa in seeds of Mucuna pruriens and its herbal formulation. HPTLC on silica gel with n-butanol - glacial acetic acid - water 5:1:4. Densitometric evaluation at 280 nm. The hRf value of levodopa was 39. The method was linear in the range of 100-1000 ng/band. The method is suitable for estimation of levodopa in herbal formulation.

Rec. Nat. Prod. 3/4, 178-186 (2009). An HPTLC method has been reported for quantitative estimation of alpha-mangostin in fruit pericarp of Garcinia mangostana (Hypericaceae). HPTLC on silica gel with chloroform - methanol 9:1 in a saturated chamber (10 min). The plate was derivatized with anisaldehyde sulfuric acid reagent. Densitometric quantification at 382 nm. Alpha-mangostin was detected as a compact band with hRf value of 46. The method was linear in the range of 1-5 µg/band. The maximum yield of alpha-mangostin was obtained from the plant by hot extraction with methanol.