Pharmacogn. Mag. DOI: 10.1177/09731296231189619 (2023). HPTLC of naringenin (1), ferulic acid (2) and caffeic acid (3) in Nyctanthes arbor-tristris on silica gel with toluene - ethyl acetate - formic acid 6:4:1. Quantitative determination by absorbance measurement at 371 nm. Linearity was in the range of 100-4000 ng/zone. Intermediate precisions were below 3 % (n=3). The LOD and LOQ were 30 and 45 ng/zone for (1), 15 and 90 ng/zone for (2) and 17 and 52 ng/zone for (3), respectively. Recovery was in the range of 86.7-96.3 % for (1), 101.6-102.7 % for (2) and 100.8-101.8 % for (3).

J. Sep. Sci. 46, 2300582 (2023). HPTLC of berberine in Berberis vulgaris, Berberis
aquifolium, and Hydrastis canadensis on silica gel with n-propanol - formic acid - water 95:1:4 (1) and 90:1:9 (2). Detection of (1) by spraying with dragendorff reagent, followed by drying at 100 °C. Qualitative identification under UV light at 254 and 366 nm. The hRF values for berberine in different plants were between 44 and 49.

Chinese J. Ethnomed. & Ethnopharm. 32 (22), 42-46 (2023). Sophora alopecuroides is a medicinal plant growing in Northern China, containing alkaloids, flavonoids, volatile oils and amino acids. It has antipyretic detoxification, antibacterial and anti-inflammatory, as well as analgesic effects, and is the main effective ingredient in some TCM mixtures widely used in clinical practice. This research team developed a medicinal gel, Kudouzi Ningjiaoji gel, which has good clinical results in the treatment of acne, pustules and other diseases. For quality control, TLC of the extracts of Kudouzi Ningjiaoji gel on silica gel with dichloromethane – methanol – ammonia water 50:6:1. Detection in daylight after spraying with a solution of bismuth potassium basic nitrate 0.85 g, glacial acetic acid 10 mL, water 40 mL and potassium iodide 2 g. Identification by comparing of sample fingerprints with those standards sophoridine and matrine.

J. Ethnopharmacol. 319, 117085 (2024). HPTLC of betaine in Sida cordifolia on silica gel with methanol - ammonia 3:1. Detection by dipping into modified Dragendroff’s reagent, followed by heating at 120 °C for 20 min. Qualitative analysis under UV light at 540 nm.

J. Ethnopharmacol. 318, 117004 (2024). HPTLC of gallic acid in Pyracantha crenulata on silica gel with toluene - ethyl acetate - formic acid 8:10:3. Qualitative identification under UV light at 254 nm. The hRF value of gallic acid was 74. 

J. Ethnopharmacol. 321, 117566 (2024). HPTLC of Evolvulus alsinoides on silica gel with toluene - ethyl acetate - formic acid 6:3:1. Qualitative identification under UV light between 450 and 550 nm. The hRF values of methanol extract ranged from 3 to 64. 

J. Ethnopharmacol. 321, 117566 (2024). HPTLC of friedelin in Euphorbia grantii on silica gel with hexane – ethyl acetate 19:1. Detection by spraying with para anisaldehyde, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 580 nm. LOD and LOQ were 0.5 and 1.8 μg/zone, respectively. 

J. Chinese Pharm. 34 (1), 52-56 (2023). Inonotus obliquus is a new source of fungal medicine, containing polysaccharides, polyphenols, triterpenoids, sterols and folic acid derivatives, with various pharmacological effects such as anti-tumor, hypoglycemic, antioxidant, and antiviral. For identification, TLC of its extracts on silica gel, A) for thrombotic acid, with chloroform – toluene – ethyl acetate – methanol 10:4:1:1; and B) for inotodiol, with dichloromethane – toluene – ethyl acetate – methanol 100:40:3:3. Detection for both by spraying with 5 % phosphomolybdate in ethanol and heating at 105°C until the zones are visible in daylight. Identification by fingerprint comparison with the standard drug Inonotus obliquus and the standards thrombotic acid and inotodiol. Chemical pattern recognition analysis was applied on resulting TLC and HPLC data (such as, cluster analysis, principal component analysis, orthozonal least squares-discriminant analysis) to evaluate the quality of the medicine.