Rev. Bras. Farmacogn. 27, 50-53 (2017). HPTLC of quercetin (1) and gallic acid (2) in Leea indica on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 63 and 45, respectively. Linearity was between 200 and 1000 ng/zone for (1) and between 200 and 1200 ng/zone for (2), respectively. The intermediate precision was <2 % (n=3). The LOD and LOQ were 21 and 65 ng/zone for (1) and 15 and 55 ng/zone for (2). Recovery rate was in the range of 98.0-99.1 % for (1) and 99.3-100.2 % for (2).

J. Planar Chromatogr. 30, 259-263 (2017). HPTLC of lupenone in the barks of D. melanoxylon on silica gel with n-hexane ‒ ethyl acetate 41:9. Quantitative determination by absorbance measurement at 395 nm. The hRF value for lupenone was 31. Linearity was between 100 and 500 ng/zone. The intermediate precision was <2 % (n=6). LOD and LOQ were 40 and 100 ng/zone. Average recovery rate was 98.7 %.

J. Ethnopharmacol. 213, 230-255 (2018). Review of the botany, ethnopharmacology, phytochemistry, biological activities, nutritional value, possible molecular mechanisms, safety and clinical applications of Morinda officinalis with a special focus on its bioactivities, including the application of HPTLC for the analysis of oligosaccharides from different habitats.

and Glycyrrhiza uralensis Fisch.) by HPTLC, validated by HPLC and DNA sequencing. J. Planar Chromatogr. 30, 467-473 (2017). HPTLC of 18-β-glycyrrhizic acid in two licorice root species (Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch) on silica gel with dichloromethane – methanol – water – formic acid 120:75:15:1 with chamber saturation for 20 min to a migration distance of 70 mm. Quantitative determination by absorbance measurement at 254 nm. Detection of species by immersion into sulfuric acid reagent, followed by heating at 100 °C for 10 min, evaluation under UV 366 nm and white light. The HPTLC results correlate with the data obtained by HPLC and by DNA sequencing.

J. Planar Chromatogr. 31, 230-234 (2018). HPTLC of hypoxoside in the roots of Hypoxis hemerocallidea on silica gel with chloroform – methanol – water 35:15:1. Quantitative determination by absorbance measurement at 257 nm. The hRf value for hypoxoside was 30. Linearity was in the range of 0.02-1.80 µg/mL. The LOD and LOQ for hypoxoside were 0.51 µg and 1.65 µg/mL. (Note by the editor: It can not be true that the start of calibration is below the LOD by a factor of 25.) The intermediate precision was below 5 %. Average recovery was 84 %.

– a botanical source of the Ayurvedic drug Jivanti. J. Planar Chromatogr. 31, 150-154 (2018). HPTLC of β-sitosterol (1), lupeol (2), and oleanolic acid (3) in Leptadenia pyrotechnica with toluene – ethyl acetate 9:4 for (1); toluene – ethyl acetate – formic acid 70:30:3 for (2), and toluene – methanol – formic acid 45:20:1 for (3). Detection by spraying with freshly prepared p-anisaldehyde sulfuric acid reagent, followed by heating at 110 °C for 3 min. Quantitative determination by absorbance measurement at 530 and 540 nm. The hRf values for (1) to (3) were 64, 84 and 47, respectively. Linearity was in the range of 2-10 μg/zone for (1) to (3). The intermediate precision was below 0.6 %. The LOD and LOQ were 410 and 1270 ng/zone for (1), 550 and 1680 ng/zone for (2) and 300 and 920 ng/zone for (3), respectively. Average recovery for (1) to (3) was 99 %.

Pharmacogn. Mag. 14, 619-629 (2018). HPTLC of_x000D_ ferulic acid (1), chalcone (2), and catechin (3) in Saraca asoca extract on silica gel with toluene – ethyl acetate – formic acid – methanol 15:15:4:1. Qualitative evaluation at UV 280 nm. The hRF values for (1) to (3) were 79, 88 and 67, respectively.

J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.