Planta Med. 70, 551-555 (2004). Analytical TLC of withaphysalin F and two new epimeric withaphysalins ((17S,20R,22R)-5beta,6beta:18,20-diepoxy-4beta,18-dihydroxy-1-oxo-witha-24-enolide (18R and 18S)) on silica gel with chloroform - ethyl acetate 3:7. Detection by spraying with 10 % sulfuric acid in ethanol, followed by heating at 120 °C for 5 min (orange spots).

Planta Med. 72, 187-189 (2006). Preparative TLC of shinpherocarpin, 7,4’-dihydroxy-2’,5’-dimethoxyisoflav-3-ene, and 7-hydroxy-4’-methoxy-3’-prenylisoflavone (5-deoxy-3’-prenylbiochanin A) on silica gel with hexane - dichloromethane 3:7 (multiple development). Detection under UV light at 254 nm.

J. Planar Chromatogr. 19, 267-272 (2006). TLC of chelerythrine, chelidonine, and sanguinarine on silica gel with dichloromethane - methanol 97:3. Use of the mobile phase recommended by Ph. Eur. 5, propanol - water - formic acid 90:9:1, did not enable satisfactory separation of Chelidonium alkaloids. Development at 20 - 24 °C and 60 % relative humidity in a presaturated TLC chamber. After drying evaluation under UV light at 254 and 366 nm.

LC-GC Europe, The Applications 19, (2006). HPTLC of Actaea racemosa (black cohosh) extracts on silica gel with toluene - ethyl formate - formic acid 5:3:2 with chamber saturation for 20 min and at a relative humidity of 5 % (automatic development chamber ADC2 with molecular sieve). Detection by dipping in sulfuric acid reagent (20 mL of sulfuric acid in 180 mL of methanol) followed by heating at 100 °C for 5 min. Evaluation under daylight and under UV 254 and 366 nm. The method is specific for identification of black cohosh and for discrimination from different species of common adulterants.

Studied by Different Chromatographic Techniques. Chromatographia 63 (Supplement 13), S81 - S86 (2006). TLC of isoquinoline alkaloids (chelidonine, chelerythrine, sanguinarine, coptisine and berberine) in Chelidonium plant organs on silica gel with chloroform - methanol 2:1, and methylene chloride - methanol 97:3. Quantification by densitometry at UV 254 nm. Detection is very sensitive because of fluorescence of alkaloids without purification. Comparison with HPLC, showing that the TLC methodis the most simple, accurate, reproducible and convenient analytical technique for fast investigations and routine determination of Chelidonium alkaloids.

Pharmazie 60, 155-159 (2005). Preparative TLC of stenocephalin A, stenocephalin B, and 5,6-dimethoxy-2-isopropenylbenzofuran on silica gel with petroleum ether - acetone 5:2, and chloroform - acetone 40:1 and 20:1. Detection by spraying with 5 % sulfuric acid in ethanol followed by heating. Evaluation under UV light.

Pharmazie 60, 461-463 (2005). Preparative TLC of ent-manool-13-O-beta-D-4’-acetylxylopyranoside and ent-manool-13-O-beta-D-3’-actylxylopyranoside on silica gel by three fold development with chloroform - ethyl acetate 30:1. Detection by spraying with 5 % sulfuric acid in ethanol or 5 % iron(III) chloride in ethanol, followed by heating. Evaluation under UV light.

J. Planar Chromatogr. 20, 61-64 (2007). HPTLC of flavonoids (catechin, epicatechin, gallocatechin, catechin gallate as standards) on RP-18 with acetonitrile - water - formic acid 10:40:3. The best separation of catechin and epicatechin was achieved by multiple gradient development with increasing concentrations of acetonitrile (from 20 to 22%) in the water - formic acid mixture. UV detection at 282 and 500 nm (after derivatization with vanillin-phosphoric acid) for estimation of catechin content.