J. of China Pharm. 21 (10), 26-27 (2012). Yifeitongluo granules are a herbal TCM preparation for the combined treatment of deficiency of both qi and yin. TLC of the extracts of the preparations on silica gel 1) for Rhizoma Polygonati, with chloroform – methanol – acetic acid 5:4:1, detection by spraying with 2.5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible; 2) for Viola yedonensis Makino, with toluene – ethyl acetate – formic acid 5:3:1, detection under UV 366 nm; 3) for Stemona sessilifolia (Miq.) Miq, with toluene – propanone – methanol 16:6:1 after preconditioning with ammonia vapors, detection by spraying with 5 % potassium iodobismuthate and viewing in daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22(1), 133-134 (2011). Capparis spinosa L. is a plant with an important ecological function which is mainly growed in the sand of the Gobi desert. It has a huge root system which helps to maintain the humidity of the land. As a Uygur traditional medicinal herb, it is effective in curing diseases like rheumatism, toothache, and dysentery. However, hitherto only Capparis spinosa root has been used in the preparations. This wrecks seriously the resource of the herbal drug and its ecological function. Therefore, its fruits have recently been applied as replacement for the root in the preparations. Quality control of the fruits by TLC on silica gel with chloroform – methanol – formic acid – water 20:90:10:3. Detection by spraying with 5 % potassium iodobismuthate solution. The fingerprints were compared with the extracts from the root, taking one of the typical components, betaine, as the marker substance. In addition the fruits were identified based on their shape, size, surface colors, smell, and the sarcocarp; by microscopy and by determination of the moisture content.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (5), 1045-1048 (2011). Dieda Zhitong microemulsion spray is a traditional Chinese medicine effective in invigorate the circulation of blood, relieving blood stagnate, subsidence of a swelling, stop bleeding and relieving pain caused by injuries from falls, fractures, contusions, strains and traumatic injuries. For quality control, TLC of the extracts of the medicine 1) for Cnidium monnieri L. Cuss and Doubleteeth Pubescent Angelica root, on silica gel with petroleum ether (30-60 ºC) – ethyl acetate 9:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visible, viewing under UV 366 nm; 2) for Ligusticum wallichii and Rheum officinale on silica gel with petroleum ether (30-60 ºC) – ethyl acetate 9:1, detection under UV 254 nm; 3) for Oleum Ocimi gratissimi on silica gel with cyclohexane – ethyl acetate 4:1, detection by spraying with 10 % ferric chloride in ethanol and heating at 105 °C until the zones were visible; 4) for Oleum Anisi stellati on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 19:1, detection by spraying with 5 % vanillin in ethanol and heating at 105 °C until the zones were visible; 5) for Oleum Eucalypti on silica gel with cyclohexane – diethyl ether - ethyl acetate 16:3:1, detection by spraying with 5 % vanillin in ethanol and heating at 105 °C until the zones were visible.

J. of Qilu Med. & Pharm. 31 (6), 339-340 (2012). Yinxie Granules are a herbal TCM for treating difficult skin diseases, like psoriasis, etc. For quality control, identification of Smilax china L. by TLC on silica gel with chloroform – ethyl acetate – methanol – water 3:8:3:2, detection under UV 366 nm. Quantification of astilbin by HPLC.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (8), 2001-2002 (2011). Monkshood and Radix Aconiti agrestis, as traditional Chinese herbal drugs, are usually processed into small pieces ready for decoction and are clinically effective for relieving rheumatic pains. However, in the market some processed drugs were found to be mixed with counterfeits. Presentation of an assay method to investigate the situation of adulteration of these drugs. TLC of the extracts of the drugs 1) for alkaloids, on silica gel with n-hexane – ethyl acetate – methanol 32:18:5 with chamber saturation with ammonia vapors for 20 min, detection by spraying with 5 % potassium iodobismuthate solution. Identification a) by fingerprint comparison with the individual standard component drug; b) by microscopy distinguishing the cluster crystal of calcium oxalate consisted in the thin-wall cells of the drug; 2) for peoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 40:5:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C.

Chinese J. Mod. Drug Appl. 6 (9), 124-125 (2012). Danbaijing capsules are a herbal TCM for treating intractable proteinuria. For quality control, TLC on silica gel 1) for Radix Astragali and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Centella asiatica and the standard asiaticoside, with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Strait Pharm. 24 (9), 53-55 (2012). Huoxiang Qushu Soft capsules are an approved herbal TCM preparation used for promoting perspiration, reducing internal heat, regulating spleen and stomach. For quality control, TLC on silica gel 1) for Herba Pogostemonis, with petroleum ether (30-60 ºC) – ethyl acetate – glacial acetic acid 85:15:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Angelicae Dahuricae, with petroleum ether (30-60 ºC) – diethyl ether 3:2, detection under UV 366 nm; 3) for Folium Perillae, with ethyl acetate – methanol – formic acid – water 18:1:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 4) for Rhizoma Atractylodis, with petroleum ether (30-60 ºC) – acetone 9:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 5) for Rhizoma Pinelliae Preparatum, with petroleum ether (30-60 ºC) – ethyl acetate – acetone – formic acid 60:12:10:1, detection under UV 254 nm; 6) for Poria, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight; 7) for Radix Glycyrrhizae, with ethyl acetate – formic acid – glacial acetic acid – water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.

Phytochem. Anal. 25, 29-35 (2014). HPTLC of hyoscyamine (1), scopolamine (2) and their biosynthetic precursors anisodamine (3), cuscohygrine (4) and litorine (5) in selected Solanaceae plants on silica gel with two-stage multi-development using chloroform - methanol - acetone - 15:3:2 + 1 drop ammonia. Quantitative determination by absorbance measurement at 190 nm for (3) and at 520 nm for (1), (2), (4) and (5) after spraying and derivatising with Dragendorff’s reagent following a Munier and Macheboeuf modi?cation (Baerheim-Svendsen and Verpoorte, 1983). The hRf values for compounds (1) to (5) were 41, 78, 24, 30 and 49, respectively. Linearity was in the range of 1000-3000 ng/zone for (3) and 500-3000 ng/zone for (1), (2), (4) and (5). LOD and LOQ were 127 and 425 ng/zone for (1), 100 and 334 ng/zone for (2), 11 and 38 ng/zone for (3), 104 and 413 ng/zone for (4), and 111 and 373 ng/zone for (5), respectively. Recoveries were in the range of 88-91 % for all the analytes. Intermediate intra- and inter-day precision was below 3 % (n=3).