Chinese J. Mod. Drug Appl. 6 (9), 124-125 (2012). Danbaijing capsules are a herbal TCM for treating intractable proteinuria. For quality control, TLC on silica gel 1) for Radix Astragali and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Centella asiatica and the standard asiaticoside, with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Strait Pharm. 24 (9), 53-55 (2012). Huoxiang Qushu Soft capsules are an approved herbal TCM preparation used for promoting perspiration, reducing internal heat, regulating spleen and stomach. For quality control, TLC on silica gel 1) for Herba Pogostemonis, with petroleum ether (30-60 ºC) – ethyl acetate – glacial acetic acid 85:15:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Angelicae Dahuricae, with petroleum ether (30-60 ºC) – diethyl ether 3:2, detection under UV 366 nm; 3) for Folium Perillae, with ethyl acetate – methanol – formic acid – water 18:1:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 4) for Rhizoma Atractylodis, with petroleum ether (30-60 ºC) – acetone 9:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 5) for Rhizoma Pinelliae Preparatum, with petroleum ether (30-60 ºC) – ethyl acetate – acetone – formic acid 60:12:10:1, detection under UV 254 nm; 6) for Poria, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight; 7) for Radix Glycyrrhizae, with ethyl acetate – formic acid – glacial acetic acid – water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.

Phytochem. Anal. 25, 29-35 (2014). HPTLC of hyoscyamine (1), scopolamine (2) and their biosynthetic precursors anisodamine (3), cuscohygrine (4) and litorine (5) in selected Solanaceae plants on silica gel with two-stage multi-development using chloroform - methanol - acetone - 15:3:2 + 1 drop ammonia. Quantitative determination by absorbance measurement at 190 nm for (3) and at 520 nm for (1), (2), (4) and (5) after spraying and derivatising with Dragendorff’s reagent following a Munier and Macheboeuf modi?cation (Baerheim-Svendsen and Verpoorte, 1983). The hRf values for compounds (1) to (5) were 41, 78, 24, 30 and 49, respectively. Linearity was in the range of 1000-3000 ng/zone for (3) and 500-3000 ng/zone for (1), (2), (4) and (5). LOD and LOQ were 127 and 425 ng/zone for (1), 100 and 334 ng/zone for (2), 11 and 38 ng/zone for (3), 104 and 413 ng/zone for (4), and 111 and 373 ng/zone for (5), respectively. Recoveries were in the range of 88-91 % for all the analytes. Intermediate intra- and inter-day precision was below 3 % (n=3).

Chinese J. of Med. Guide 11 (1), 470-472 (2013). Shenshi Keli granule is a herbal TCM preparation for the treatment of urinary calculus. For quality control TLC on silica gel 1) for Dichondra repens Forst., with chloroform – acetone 19:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min and viewing under UV 366 nm; 2) for Chicken gizzard’s stomach lining, with n-butanol – glacial acetic acid – water 7:1:1, detection by spraying with 0.3 % ninhydrin in n-butanol and heating at 105 °C until the zones are visible in daylight.

J. of China Pharm. 21 (15), 49-50 (2012). Tongfenglishi Koufuye oral liquid is a herbal TCM preparation for the treatment of rheumatic arthralgia, the numbness of limbs, swollen carbuncles and hyperlipidemia. For quality control, TLC on silica gel 1) for Radix et Rhizoma Rhei, with petroleum ether (30-60°C) – ethyl formate – formic acid 15:5:1, detection in daylight; 2) for Fructus Crataegi, with cyclohexane – chloroform – ethyl acetate 20:5:8, detection by spraying with 10 % sulfuric acid in ethanol, heating at 110 °C and viewing in daylight; 3) for Semen Vaccariae, with chloroform – methanol – water 15:7:2, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and viewing in daylight. Quantification of emodin by HPLC.

J. of China Pharm. 26 (6), 620-622 (2012). Rheum palmatum L., a herbal TCM drug for clearing away body heat, eliminating toxin and nourishing skin, is commonly used as a component of herbal TCM preparations. However, a homologous herb, Rumex madaio Makino (syn. Rumex daiwoo Makino) is sometimes mistaken for Rheum palmatum in drug production. For quality control of rhaponiticin in antibacterial Kangjun Xiaoyan Jiaonang capsules, TLC of the extracts and the standard rhaponiticin on silica gel with ethyl acetate – acetone – water 10:7:1, detection under UV 366 nm. Quantification of rhaponiticin by HPLC. The method was successfully applied to nine batches of real life samples from five pharmaceutical factories.

J. Planar Chromatogr. 27, 102-106 (2014). HPTLC of betulinic acid in the leaves of Vitex negundo on silica gel with toluene - acetone - formic acid 17:3:2. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 445 nm. The hRf value for betulinic acid was 42. Linearity was in the range of 200-1200 ng/zone. The intermediate/interday/intra-day precisions were below 1.3 % (n=3). The LOD and LOQ were 6 and 18 ng/zone, respectively. Recovery was in the range of 99.7-101.5 %.

and Dendranthema indicum, on silica gel containing 1 % NaOH, with ethyl acetate – methanol – water 100:17:10, detection by spraying with 1 % aluminum trichloride in ethanol firstly, then with 5 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.