Pharmacogn. Mag. 17, 233-239 (2021). HPTLC of esculin in the leaves of Launaea pinnatifida on silica gel with chloroform - methanol - formic acid 13:6:1. Quantitative determination by absorbance measurement at 343 nm. The hRF value of esculin was 70. Linearity was between 8 and 250 µg/mL. The LOD and LOQ were 8 and 25 µg/zone. Average recovery rate was 99.8 %.

Bioorg. Chem. 88, 102947 (2019). HPTLC of pentylcurcumene in the aerial part of Geophila repens on silica gel with benzene - methanol 3:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value of pentylcurcumene was 50. Linearity was between 100 and 500 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 16 and 49 ng/zone. Recovery was between 97.2 and 98.7 %.

J. Sep. Sci. 44, 3146-3157 (2021). HPTLC of  gallic acid (1), cinnamic acid (2), piperine (3), eugenol (4), and glycyrrhizin (5) in Divya-Swasari-Vati on silica gel with ethyl acetate - toluene - formic acid 10:9:1 for (1) to (4) and ethyl acetate - formic acid - acetic acid - water 100:10:10:23 for (5). Quantitative determination by absorbance measurement at 280 nm for (1), (2) and (4), 343 nm for (3) and 254 nm for (5). The hRF values for (1) to (5) were 31, 64, 53, 70 and 29, respectively. Linearity was between 400 and 800 µg/mL for (1), 5 and 25 µg/mL for (2), 600 and 1400 µg/mL for (3), 400 and 1200 µg/mL for (4) and 100 and 800 µg/mL for (5). Intermediate precision was below 2 % (n=18). The LOD and LOQ were 180 and 560 ng/g for (1), 3 and 10 ng/g for (2), 8 and 26 µg/g for (3), 3 and 11 µg/g for (4) and 300 and 920 ng/g for (5), respectively. Mean recovery was 96.0 % for (1), 92.3 % for (2), 94.2 % for (3), 96.2 % for (4) and 94.6 % for (5). 

J. Ethnopharmacol. 276, 114144 (2021). Review of analytical methods for the analysis of the main secondary metabolites detected and isolated from Ammi majus. The paper described TLC and HPTLC methods for the determination of coumarins in fruits.

J. Ethnopharmacol. 281, 114503 (2021). Review of the reported phytochemical studies of Sesamum indicum, including TLC and HPTLC methods for the analysis of major compounds. 

J. Ethnopharmacol. 280, 114417 (2021). HPTLC of bergenin (1), epicatechin (2) and gallic acid (3) in the rhizomes of Bergenia ciliata on silica gel with toluene - ethyl acetate - formic acid 3:7:1. Quantitative determination by absorbance measurement at 280 nm. The hRF values of (1) to (3) were 32, 52 and 74, respectively.

J. Ethnopharmacol. 275, 114054 (2021). HPTLC of ellagic acid in Anogeissus latifolia on silica gel with toluene - ethyl acetate - formic acid 20:5:1. Detection by spraying with anisaldehyde - sulphuric acid reagent, followed by heating at 105 °C. The hRF value for ellagic acid was 38.

Phytochem. Anal. 3093 (2021). HPTLC of hyperforin (1), hypericin (2) and hyperoside (3) in Hypericum species on silica gel with n-hexane - ethyl acetate 4:1 for (1), toluene - chloroform - ethyl acetate - formic acid 80:50:35:6 for (2) and ethyl acetate - formic acid - acetic acid - water 15:2:2:1 for (3). Detection by spraying with a derivatizing reagent (20 mL of sulfuric acid were carefully added to 120 mL methanol and diluted with 200 mL methanol), followed by heating. Quantitative determination by absorbance measurement at 366 nm. The hRF values of (1) to (3) were 49, 35 ad 49, respectively. Linearity was between 0.4 and 1.4 µg/zone for (1), 20 and 100 ng/zone for (2) and 10 and 100 ng /zone for (3). The intermediate precision was below 2 % (n=6) for (1) to (3). The LOD and LOQ were 120 and 400 ng/zone for (1), 6 and 20 ng/zone for (2) and 3 and 10 ng/zone for (3), respectively. Recovery was between 101.0 and 101.2 % for (1), 98.8 and 100.1 % for (2) and 100.4 and 101.4 % for (3).