Journal of Pharmacy Research 3(5), 1107-1109 (2010). HPTLC of mangiferin in Salacia chinesis (Hippocrateaceae) on silica gel with ethyl acetate - methanol 2:3 at 25 °C with chamber saturation for 30 min. Densitometric evaluation at 254 nm. Derivatization by spraying with acetic anhydride-sulfuric acid-ethanol reagent, followed by heating at 110 °C for 2 min. The method was linear in the range of 10-200 ng/band. The plant was found to contain 1.54 % of mangiferin.

International J. Pharm. & Tech 2(2), 285-292 (2010). TLC of gallic acid in acetone extracts of bark powder of Acacia nilotica on silica gel with toluene – ethyl acetate – formic acid 15:10:2. The hRf value of gallic acid was 36. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 100-350 ng/band with recovery of 97.5 %.

J.of Jiangxi Univ. of TCM 22 (5), 55-57 (2010). TLC of Shujinhuoxue pills: 1) for Angelica sinensis, on silica gel with cyclohexane – ethyl acetate 12:1, detection under UV 365 nm; 2) for Rheum officinale, on silica gel with petroleum ether (30-60 ºC) – ethyl formate – formic acid 15:5:1, detection by exposure to ammonia vapors; 3) for Radix Rehmanniae praeparata, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 1:1, detection under UV 254 nm; 4) for Gardenia jasminoides, on silica gel with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 ºC until the zones were visualized; 5) for Lignum Sappan, on polyamide phase with 36 % acetic acid, detection by spraying with 5 % AlCl3 in ethanol and heating mildly until the spots were visualized.

International Journal of PharmTech Research 3(2), 986-999 (2011). TLC of methanolic extracts of Haritaki and gallic acid and rutin as markers on silica gel with toluene - ethyl acetate - formic acid 3:6:1 for gallic acid and chloroform - ethyl acetate - methanol - formic acid 7:10:1:2 for rutin. Quantitative determination by densitometry in absorbance mode at 280 nm for gallic acid and 254 nm for rutin. The method was linear in the range of 100-500 ng/band for gallic acid and 1000-5000 ng/band for rutin. The recovery was 99.1 % for gallic acid and 97.9 % for rutin. The LOD and LOQ of gallic acid was 71 and 213 ng/zone and of rutin 63 and 189 ng/zone.

PHCOG J. 2(11), 381-385 (2010). TLC of Tylophora indica leaves, extracted with petroleum ether, chloroform and methanol, on silica gel with 1) n-hexane - ethyl acetate 2:3 for the petroleum ether extract, 2) chloroform - ethyl acetate - methanol 18:1:1 for the chloroform extract, and 3) chloroform - toluene - ethyl acetate 18:1:1 for the methanolic extract. Evaluation under UV 254 nm and 366 nm. 12 prominent bands were observed in all chromatographic fingerprints. The method is suitable for identification and authentication of the plant material.

J. Planar Chromatogr. 24, 116-120 (2011). HPTLC of rosin, rosarin, and rosavin in the roots of Rhodiola rosea L. and Rhodiola sachalinensis Borissova on silica gel, prewashed with methanol, with chloroform - methanol - water 130:70:21 in a twin-trough chamber lined with filter paper and saturated for 20 min, at 21-24 °C and 40-45 % relative humidity. Quantitative determination by densitometry at 250 nm. The calibrations were linear in the range of 100-500 ng/band with correlation coefficients of 0.9999, 0.9992, and 0.9992 for rosin, rosarin, and rosavin, respectively. Recovery of the three compounds was between 97 and 101 %. The LOD and LOQ were 30 and 100 ng/band, respectively, for all three compounds. The hRf values for rosin, rosarin, and rosavin were 58, 45, and 42, respectively. Intra-day variation, as %RSD, was 2.7 % for rosin, 1.7 % for rosarin, and 2.8 % for rosavin, with standard errors of 0.3 %, 0.2 %, and 0.3 %, respectively. Inter-day variation, as % RSD, was 4.8 % for rosin, 4.2 % for rosarin, and 3.9 % for rosavin, with standard errors of 0.5 %, 0.5 %, and 0.4 %, respectively.

J. Liq. Chromatogr. Relat. Technol. 32, 2893-2905 (2009). HPTLC of albiflorin, paeoniflorin, (beta)-catechin, benzoyloxypaeoniflorin, benzoylpaeoniflorin, beta-sitosterol in the roots of Radix paeoniae Rubra (1) and Radix Paeoniae Alba (2) on silica gel with chloroform - ethyl acetate - methanol - formic acid 30:5:10:1 for high polarity components and toluene - ethyl acetate - methanol - formic acid 20:4:2:1 for lipophilic components. Detection by spraying with vanillin - sulphuric acid - ethanol 1:5:95, followed by heating at 105 ºC for 10 min. Qualitative determination by densitometry at 366 nm. The HPTLC fingerprints allowed differentiation between the roots of (1) and (2).

Research Journal of Pharmaceutical, Biological and Chemical science 2(12), 389-396 (2011). TLC of a polyherbal formulation containing Mucuna pruriens with L-dopa as biological marker on silica gel with n-butanol - water - glacial acetic acid 4:1:1 with chamber saturation for 30 min. Detection by dipping in a 0.5 % solution of ninhydrin in ethanol, followed by heating at 120 °C for 2 min. Quantitative determination of L-dopa by densitometry in absorbance mode at 520 nm. The hRf value of L-dopa was 37. Linearity was given in the range of 600-1400 ng/zone.