J. Planar Chromatogr. 18, 319-322 (2005). HPTLC of emodin and phenolic acids (protocatechuic, homoprotocatechuic, caffeic, syringic, vanillic, ferulic, p-hydroxyphenylacetic, alpha-resorcylic, p-coumaric, gallic and ellagic acid) on silica gel in horizontal chambers with toluene - dichloromethane - ethyl acetate 4:4:1. Also two-dimensional TLC of phenolic acids on cellulose with benzene - methanol - acetic acid - acetonitrile 16:2:1:1 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. After drying the chromatograms were observed under UV light at 254 nm before and after treatment with ammonia vapor. Derivatization was performed by spraying with either diazotized sulfanilic acid in 20 % sodium carbonate solution or with 2 % aqueous iron(III) chloride. Detection limits between 10 and 64 ng. Videodocumentation and quantitation by densitometry.

The Pharma Review, Dec, 106-108 (2005). HPTLC of hexane, petroleum ether, chloroform, and methanol extracts of Arnebia nobilis (Ratanjot) roots on silica gel with n-hexane - methanol 9:1 and toluene - chloroform - methanol 14:5:1. Detection by spraying with anisaldehyde sulphuric acid reagent and densitometric fingerprint analysis by absorbance measurement at 366 nm. HPTLC fingerprinting profile provided the most reliable method for correct identification of the root.

Planta Med. 70, 834-840 (2004). Analytical and preparative TLC of flavonoids (lavandulifolioside, plantamajoside, acteoside, leucosceptoside, and martynoside) on silica gel, RP18, polyamide and cellulose. E. g. 2D-TLC on cellulose with n-butanol - acetic acid - water 4:1:5 in the first and acetic acid - water 15:85 in the second direction, preparatice TLC on polyamide with ethyl acetate - ethanol - water 20:3:2 (2 x), 50:3:10, and 25:3:3 (4 x). Detection under UV light at 366 nm before and after spraying with 0.1 % diphenylboric acid 2-aminoethylester (natural products reagent) or 1 % aluminium chloride solution in ethanol.

J. Chromatogr. A 1112 (1-2), 165-170 (2006). HPTLC of a herbal medicinal product containing 250 mg of Aesculus hippocastanum dry extract, 120 mg of Vitis vinifera dry extract and 50 mg of excipients. After purification with C18 SPE cartridges, HPTLC on silica gel with the upper layer of a mixture of acetic acid - water - butanol 1:4:5. Detection by spraying with anisaldehyde reagent followed by heating the plate for 5-10 min at 100-105 °C. Quantitative determination by measurement at 535 nm. The method was developed to analyze the total saponin content (also referred to as the aescin content) and is applicable for the quality control and stability investigation of both the Aesculus dry extract and HMP capsules thereof containing Vitis dry extract in combination with the Aesculus dry extract. The method was validated according to the International Conference on Harmonization (ICH) guidelines. The proposed assay method is specific for aescin in the presence of Vitis dry extract and formulation excipients. Analysis of stressed samples in forced degradation tests proves the method to be applicable for stability evaluation. The standard aescin curve is linear (r > 0.99) over a concentration range of 0.16–0.80 µg/spot. Recovery from the HMP capsules is statistically equal to 100 %. The precision of the method with respect to time and concentration is acceptable, with relative standard deviation values of 1.28 and 1.49 %, respectively.

Planta Med. 71, 54-58 (2005). Preparative TLC of microphyllaquinone and (1aS*,1bS*,7aS*,8aS*)-4,5-dimethoxy-1a,7a-dimethyl-1,1a,1b,2,7,7a,8,8a-octahydrocyclopropa[3,4]cyclopenta[1,2-b]naphthalene-3,6-dione on silica gel with hexane - ethyl acetate 7:3 and dichloromethane - chloroform 7:3. Detection under UV light at 250 nm and by spraying with a solution of vanillin - perchloric acid - ethanol, followed by heating at 100 °C for 5 min.

Planta Med. 71, 12-19 (2005). TLC of petasin and isopetasin on silica gel without chamber saturation with toluene - ethyl acetate 93:7. Detection with anisaldehyde - sulfuric acid reagent [anisaldehyde - 100 % acetic acid - methanol - sulfuric acid 1:20:170:10 followed by heating at 160 °C for 1.5 min. Observation under visible and UV light at 365 nm.

Planta Med. 70, 1011-1014 (2004). TLC bioautography of bellidin, bellidifolin and the respective glucosides on silica gel with chloroform - methanol - water 50:10:1 with huperzine A, galanthamin HBr, and physostigmine as reference substances.

J. Planar Chromatogr.19 , 282-287 (2006). HPTLC of ajmaline, ajmalicine, and reserpine on silica gel, prewashed with methanol, in an unsaturated twin-trough chamber with toluene - methanol 19:6. Detection by dipping in Dragendorff’s reagent. Quantitative determination by absorbance measurement at 520 nm.