J. Planar Chromatogr. 25, 403-408 (2012). HPTLC of rosmarinic acid in Salvia species on silica gel with ethyl acetate - toluene - formic acid 6:3:1. Quantitative determination by absorbance measurement at 328 nm. The hRf value of rosmarinic acid was 42. Linearity was in the range of 200-1000 ng. The coefficient of variation (%) of intra-day and inter-day precision were 1.4 and 4.5, respectively (n=9). Recovery (by standard addition) was between 82.3 and 85.9 %.

J. Planar Chromatogr. 25, 575-580 (2012). HPTLC of echioidinin-5-O-beta-D-glucopyranoside in Andrographis echioides on silica gel with chloroform - methanol 31:9. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 200-1400 ng/zone. Limits of detection and quantification were found to be 54 and 68 ng/zone, respectively. Recovery (by standard addition) was 96.7 %.

J. of Yangtze Univ. (Nat. Sci., Med.) 9 (1), 64-65 (2012) . Angelica sinensis is a herbal TCM drug widely used in the preparations for gynaecological diseases, effective in promoting blood circulation, ease of pain and against inflammations. TLC of the extracts of the preparation on silica gel with n-hexane – ethyl acetate 9:1. Detection under UV 366 nm.

(Papaveraceae) tissues by TLC–in situ fluorography. J. Planar Chromatogr. 25, 358-360 (2012). TLC of berberine and sanguinarine on silica gel with n-butanol - water - ammonia 8:1:1. Detection by densitometry in fluorescence mode at 325 nm. The hRf of berberine and sanguinarine were 29 and 95, respectively.

J. of China Pharm. 21 (12), 15-16 (2012). The leaf of Microcos paniculata L. is a popular medicinal herbal drug used for cardiovascular protection, blood fat adjusting, and anti-aging. Presentation of methods for identifying the drug by TLC and FTIR. TLC of the extracts of the drug on silica gel 1) for kaempferide, with toluene – ethyl acetate – formic acid 9:3:1, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm; 2) for vitexin and isovitexin, developed with ethyl acetate – methanol – water 100:17:13, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm. The methods have been applied to 15 batches of real life sample available from different places of origin, and proved that TLC procedures as well as FTIR are simple, sensitive, reproducible and robust and suitable for the identification of the herbal drug.

J. Liq. Chromatogr. Relat. Technol. 35, 1565-1584 (2012). HPTLC of negundoside (1), ursolic acid (2), eugenol (3), lupeol (4), and beta-sitosterol (5) in the leaves of Vitex Negundo on silica gel with toluene - methanol 9:1. Detection by dipping in anisaldehyde sulfuric acid reagent for approximately 1 min, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 525 nm. The hRf value of compounds (1) to (5) were 47, 61, 56, 54 and 38, and selectivity regarding matrix was given. Linearity was in the range of 200-800 ng/zone for (1), 72-576 ng/zone for (2), 200-1000 ng/zone for (3), 150-900 ng/zone for (4) and 80-480 ng/zone for (5). Limits of detection and quantification were 80 and 200 ng/zone for (1), 18 and 72 ng/zone for (2), 60 and 200 ng/zone for (3), 50 and 100 ng/zone for (4) and 20 and 60 ng/zone for (5), respectively. The method provides acceptable intra-day and inter-day precision for (1) to (5). The average recoveries for compounds (1) to (5) were found to be 99.9, 100.1, 99.7, 100.0, and 99.9 %, respectively.

J. Sep. Sci. 36, 2373-2378 (2013). HPTLC of 6alpha,7alpha-diacetoxy-13-hydroxy-8(9),14-labdadien (1), 13-hydroxy-5(10),14-halimadien-6-one (2), and 9-hydroxy-13(14)-labden-16,15-olide (3) in the leaves of Vitex trifolia L. on silica gel with chloroform - acetone 49:1. Detection by spraying with vanillin-sulfuric acid reagent. Quantitative determination by absorbance measurement at 610 nm. The hRf of compounds (1), (2) and (3) were 74, 57 and 51, respectively. Linearity was 333-1000 ng/zone for (1) and (2) and 670-2000 ng/zone for (3). LOD were in the range of 73, 56, and 92 ng/zone and LOQ were found to be 242, 186, and 307 ng/zone for (1), (2) and (3), respectively. The intra- and inter-assay precisions were in the range of 1.0-1.2 % and 1.1-1.3 %, respectively for compounds (1) to (3). Recovery (by standard addition) ranged 99.1-102.7 %.

J. of Jiangxi Univ. of TCM 24 (3), 54-57 (2012). Shenbao Heji compound oral liquid is a herbal TCM for the treatment of impotence, nocturnal emission, low back pain, lack of energy, aversion to cold, and women with menorrhagia. For quality control, TLC on silica gel 1) for Epimedium davidii Franch and the standard icariine, with ethyl acetate – methanol – water 100:17:13, detection by spraying with 1 % aluminium chloride in ethanol and heating at 105 °C, viewing at UV 254 nm; 2) for Radix Polygoni Multiflori and the standard emodin, with petroleum ether (60-90 °C) - ethyl formate – formic acid 15:10:1, detection by exposing to ammonia vapors and viewing in daylight; 3) for Leguminosae and the standard astragaloside A, with the lower phase of chloroform – methanol – water 13:6:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 4) for Fructus Psoraleae and the standard psoralen, with n-hexane - ethyl acetate 3:1, detection by spraying with 10 % KOH in methanol and viewing under UV 366 nm; 5) for Radix Angelicae Sinensis and Rhizoma Ligustici Chuanxiong, with n-hexane – ethyl acetate 9:1, detection under UV 366 nm. Quantification of icariine by HPLC.