Rev. Bras. Farmacogn. 25, 193-198 (2015). HPTLC of mangiferin (1) and scopoletin (2) in Canscora decussata on silica gel with ethyl acetate - acetic acid - formic acid - water 20:1:1:3. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 22 and 78, respectively. Linearity was in the range of 100-600 ng/zone for (1) and (2). LOD and LOQ were 46 and 94 ng/zone for (1) and 31 and 78 ng/zone for (2). The intermediate precision was below 0.2 % (n=3). Average recovery was 100 % for both (1) and (2).

J. AOAC Int. 98, 871-875 (2015). TLC direct bioautography of 18 thyme (Thymus) specimens and species on silica gel with methanol - water 27:73. Bioautography by dipping into a suspension of Bacillus subtilis for 8 s, followed by incubation for 17 h at 37 °C. The plates were sprayed with 0.2 % aqueous solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and incubated for 30 min at 37 °C. Thymus citriodorus and Thymus marschallianus were selected as promising targets for further investigations.

Food Chem. 214, 162-168 (2017). HPTLC of cinnamic acid (1), ferulic acid (2), caffeic acid (3) and flavonoid-glycosides (4) in the grains of Kodo millet (Papsalum scrobiculatum) on silica gel with ethyl acetate – formic acid – water 30:2:2. Detection by exposure to iodine vapor. The hRF values for (1) to (4) were 52, 15, 7 and 10, respectively.

CBS 116, 2-4 (2016). HPTLC of steroid alkaloid saponins from potato sprouts on silica gel with chloroform – methanol – water 60:40:9 with chamber saturation without filter paper over a migration distance of 70 mm. Detection by dipping half of the plate in anisaldehyde-sulfuric acid reagent, drying for 10 min and heating at 70 °C for 5 min. The other half of the plate was used for the blood-gelatin test: dipping in 0.5 % polyisobutylmethacrylate in n-hexane – chloroform 20:1 for 10 s, coating with 40 mL blood-gelatin (2 % gelatin in PBS buffer pH 7.4 with 3 % human blood preservation), storage for 12-48 h at 4 °C. Evaluation under UV 366 nm and white light. Semiquantitative evaluation of the hemolytic activity using the substance digitonin from potato sprouts. The hemolytic activity of α-chaconine was comparable to the effect of 16-20 µg digitonin.

J. Ethnopharmacol. 194, 30-56 (2016). Review of information on the ethnobotany, phytochemistry, pharmacological research and toxicity of Anogeissus species, including the application of HPTLC for the quantitative investigation of gallic acid, ellagoc acid as well as flavonoids like quercetin and rutin.

Planta Medica 82(17), 1482-1486 (2016). TLC of the methanolic percolate of Elettaria cardamomum seeds on silica gel (with F254) with chloroform – ethyl acetate – methanol – formic acid 6:4:2:1, allowing the identification of kaempferol, rutin and quercetin by comparison to standards.

J. of China Pharm. 23 (5), 29-31 (2014). Xiaoke Maikang Keli granule is a herbal TCM for treatment of diabetes and peripheral neuropathy etc. For quality control, TLC for (1) Achyranthes bidentata, on silica gel with chloroform – methanol 40:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, evaluation under white light; (2) Salvia miltiorrhiza, on silica gel with chloroform – acetone – formic acid 79:11:10, detection under UV 254 nm; (3) for Fructus Lycii, on silica gel with ethyl acetate – chloroform – formic acid 5:4:2, detection under UV 366 nm; (4) for Taxillus sutchuenensis, on silica gel with water-saturated toluene – ethyl formate – formic acid 5:3:1, detection by spraying with 5 % aluminum trichloride in ethanol, evaluation under UV 366 nm; (5) for Corydalis yanhusuo, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 1:1, detection by exposure to iodine vapors for a few seconds and evaluation under UV 366 nm; (5) for Astragalus membranaceus, on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm.

Planta Medica 83(01/02), 104-110 (2017). TLC on silica gel 1) to monitor the column fractionation of the chloroform partition of a methanolic maceration of Caesalpinia pulcherrima roots (eluent not given); and for preparative isolation of 2) 8,9,11,14-didehydrovouacapen-5α-ol from a subfraction of the same (with n-hexane – dichloromethane – acetonitrile 50:50:1); 3) pulcherrimin E from another subfraction (with chloroform – methanol 49:1, followed by preparative HPLC); and 4) 6β-cinnamoyl-7β-acetoxyvouacapen-5α-ol and pulcherrimin D, obtained by acetylation (in pyridine and acetic anhydride) from cinnamoyl-7β-hydroxyvouacapen-5α-ol and pulcherrimin A, respectively (eluent not given). Detection by spraying with cerium sulfate and by exposure to iodine vapor._x000D_
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