Chinese J. of Med. Guide 11 (1), 470-472 (2013). Shenshi Keli granule is a herbal TCM preparation for the treatment of urinary calculus. For quality control TLC on silica gel 1) for Dichondra repens Forst., with chloroform – acetone 19:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min and viewing under UV 366 nm; 2) for Chicken gizzard’s stomach lining, with n-butanol – glacial acetic acid – water 7:1:1, detection by spraying with 0.3 % ninhydrin in n-butanol and heating at 105 °C until the zones are visible in daylight.

J. of China Pharm. 21 (15), 49-50 (2012). Tongfenglishi Koufuye oral liquid is a herbal TCM preparation for the treatment of rheumatic arthralgia, the numbness of limbs, swollen carbuncles and hyperlipidemia. For quality control, TLC on silica gel 1) for Radix et Rhizoma Rhei, with petroleum ether (30-60°C) – ethyl formate – formic acid 15:5:1, detection in daylight; 2) for Fructus Crataegi, with cyclohexane – chloroform – ethyl acetate 20:5:8, detection by spraying with 10 % sulfuric acid in ethanol, heating at 110 °C and viewing in daylight; 3) for Semen Vaccariae, with chloroform – methanol – water 15:7:2, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and viewing in daylight. Quantification of emodin by HPLC.

J. of China Pharm. 26 (6), 620-622 (2012). Rheum palmatum L., a herbal TCM drug for clearing away body heat, eliminating toxin and nourishing skin, is commonly used as a component of herbal TCM preparations. However, a homologous herb, Rumex madaio Makino (syn. Rumex daiwoo Makino) is sometimes mistaken for Rheum palmatum in drug production. For quality control of rhaponiticin in antibacterial Kangjun Xiaoyan Jiaonang capsules, TLC of the extracts and the standard rhaponiticin on silica gel with ethyl acetate – acetone – water 10:7:1, detection under UV 366 nm. Quantification of rhaponiticin by HPLC. The method was successfully applied to nine batches of real life samples from five pharmaceutical factories.

J. Planar Chromatogr. 27, 102-106 (2014). HPTLC of betulinic acid in the leaves of Vitex negundo on silica gel with toluene - acetone - formic acid 17:3:2. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 445 nm. The hRf value for betulinic acid was 42. Linearity was in the range of 200-1200 ng/zone. The intermediate/interday/intra-day precisions were below 1.3 % (n=3). The LOD and LOQ were 6 and 18 ng/zone, respectively. Recovery was in the range of 99.7-101.5 %.

and Dendranthema indicum, on silica gel containing 1 % NaOH, with ethyl acetate – methanol – water 100:17:10, detection by spraying with 1 % aluminum trichloride in ethanol firstly, then with 5 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.

J. Planar Chromatogr. 27, 199-203 (2014). HPTLC of myotonin in QiYi capsules (preparation with astragalus, myotonin, thunder god vine and centipede) on silica gel with petroleum ether - diethyl ether - glacial acetic acid 83:17:1. Detection by spraying with vanillin reagent (1 % in sulfuric acid), followed by heating at 105 ºC for 3 min. Quantitative determination by absorbance measurement at 365 nm. The hRf value for myotonin was 19. The intermediate/interday/intra-day precisions were below 3.4 % (n=5).

Chinese J, Mod. Drug Appl. 7 (14), 1-3 (2013). Tongbianning Pian tablet is a herbal TCM for the treatment of habitual constipation disease. For quality control, TLC on silica gel 1) for Folium Sennae, with chloroform – methanol – ammonia 190:8:1, detection by exposure to ammonia vapors under UV 366 nm; 2) for Pharbitis nil (Linn.) Choisy, with toluene – glacial acetic acid 100:1, detection by spraying with 5 % vanillin in sulfuric acid – ethyl acetate 22:1 and heating mildly until the zones are visible in daylight; 3) for Amomum microcarpum C. F. Liang et D. Fang and Amomum kravanh, with cyclohexane – glacial acetic acid 100:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zones are visible in daylight. Quantification of aloe emodin by HPLC.

J. Planar Chromatogr. 28, 42-47 (2015). HPTLC of (1) homoeriodictyol and (2) persicogenin in the methanol extract of the aerial parts of Rhus retinorrhoea and Rhus tripartita on silica gel with toluene - ethyl acetate - methanol 16:4:1. Quantitative determination by absorbance measurement at 293 nm. The hRF values of (1) and (2) were 30 and 48. Linearity was between 100 and 800 ng/zone for both, (1) and (2). The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 26 and 77 ng/zone for (1) and 31 and 92 ng/zone for (2), respectively. Recoveries were in the range of 98.9-99.5 % for (1) and 98.3-99.2 % for (2).