Phytochem. Anal. 22, 258-262 (2011). TLC of gomisin A (1), gomisin N (2) and schisandrin (3) in the fruits of Schisandrae chinensis on silica gel with toluene - ethyl acetate - formic acid 14:6:1. Quantitative determination by direct analysis in real time mass spectrometry (DART-MS). Linearity of (1) - (3) was between 0.5 and 5 nmole. The limits of detection and quantification were 60-200 pmole for (1), and 58-192 pmole for (2) and (3). Recovery (by standard addition) for (1) - (3) was between 104.0 and 120.2 %. TLC-DART-MS method provides faster and more specific quantification compared with the conventional densitometric and HPLC-UV methods.

Planta Med. 76, 133-136 (2010). TLC of mitraphylline on silica gel with (1) dichloromethane - acetone 5:4, (2) diethyl ether - ethyl acetate 1:1, and (3) dichloromethane - ethanol 19:1. The hRf value in system (1) was 83, in (2) 73, and in (3) 68. Detection by spraying with sulfuric acid - acetic acid - water 1:20:4 followed by heating at 120 °C and by Dragendorff’s reagent.

J. Planar Chromatogr. 24, 376-380 (2011). HPTLC of stem bark extracts from D. melanoxylon and lupeol and betulin on silica gel, prewashed with methanol, with ethyl acetate - hexane 9:41 with chamber saturation for 3 min at 29 +/- 4 °C and 65 +/- 5 % relative humidity. The hRf value was 46 and 25 for lupeol and betulin, respectively. Quantitative determination by densitometry in absorption mode at 560 nm for lupeol and 510 nm for betulin. Detection by derivatization with 5 % methanol-sulfuric acid reagent. The LOD and LOQ was 40 and 100 ng/zone for lupeol and 50 and 100 ng/zone for betulin, respectively. The instrument precision and repeatability (n = 6) were 0.8 and 1.3 % for lupeol and 1.1 and 1.2 % for betulin, respectively. The linearity range was 100-500 ng/zone for both lupeol and betulin. The intra-day and inter-day precision was 1.1-1.7 % and 1.3-2.0 % for lupeol and 0.8-1.9 % and 1.9-2.2 % for betulin.

Planta Med. 77, 271-276 (2011). Analytical and preparative TLC of limonoids and quinoline alkaloids (1’,2’-didehydro-7,8-dimethoxyplatydesmine, 3-chloro-8,9-dimethoxygeibalansine, dasylactone A, dasylactone B, dictamnine, 7,8-dimethoxymyrtopsine, isofraxinellone, fraxinellone, obacunone, limonoic acid, rutaevine, and rutaevine acetate) on silica gel with chloroform - methanol 30:1, 10:1 and 6:1. Detection under UV 254 nm and by spraying with 98 % sulfuric acid - ethanol 1:19 followed by heating.

Food Chemistry 132, 671-674 (2012). TLC of saponins on silica gel with n-butanol - water - acetic acid 12:2:1. Detection by dipping into a suspension of sheep erythrocytes for 20 s, then plates were taken out and held vertically for 30 s. White spots against a pink background appeared. The plate was immersed in phosphate-buffered saline for 30 s to remove excess blood on the plate surface and again held vertically for 30 min. The method is simple, specific, convenient and time saving for analysis of saponins by TLC for purification, chemoprofiling of plants, and nutraceutical applications.

J. Planar Chromatogr. 24, 130-135 (2011). TLC of Juniperus essential oils (containing terpenoids, monoterpenes, sesquiterpenes and alcohols) with guaiazulene and cineole as standards on silica gel with ethyl acetate - toluene 1:19. Detection by spraying with anisaldehyde reagent followed by heating for 5 min at 100 °C and examination under daylight. The hRf values were 42 and 89 for cineole and guaiazulene, respectively.

Asian Journal of Chemistry 23 (5), 2046-2048 (2011). TLC of alpha- and beta-asarone in Acorus calamus on caffeine modified silica gel (with 10 % caffeine in dichloromethane and dried at 100 °C for 10 min) with toluene - ethyl acetate 93:7. The hRf value of alpha-asarone was 67 and of beta-asarone 77. Quantitative evaluation by absorbance measurement at 313 nm. The linearity was in the range of 50-1000 ng/band for beta-asarone. The alcoholic extracts of samples from different geographical regions were found to contain 0.2-0.8 % of alpha-asarone and 8.7-11.2 % of beta-asarone.

J. Planar Chromatogr. 25, 394-402 (2012). 2D-HPTLC of kaempferol, quercetin, rutin, hyperoside, ferulic acid, gallic acid, caffeic acid, chlorogenic acid, chinic acid, p-coumaric acid, catechin, epicatechin, and resveratrol in the flowers of Eupatorium cannabinum on cyano phase with propan-2-ol mixed with n-heptane, and ethyl acetate mixed with n-heptane as non-aqueous mobile phases in the first direction and after turning the plate 90 ° with methanol mixed with water in the second direction of development. Detection by spraying with diphenylborinic acid 2-aminoethylester and PEG 4000 or DPPH radical reagent. Evaluation under UV 254 nm and 366 nm. The 2D-HPTLC system allowed the separation of the phenolic fractions.