J. Liq. Chromatogr. Relat. Technol. 36, 197-212 (2013). HPTLC of lupeol (1) and stigmasterol (2) in the stems of Costus igneus on silica gel with n-hexane - ethyl acetate 4:1 for (1) and toluene - acetone - acetic acid 89:9:2 for (2). Detection by dipping in a solution of 0.5 mL p-anisaldehyde in 50 mL glacial acetic acid and 1 mL of 97 % sulfuric acid, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 538 nm. The hRf values of (1) and (2) were 55 and 58, respectively. Linearity was in the range of 5-10 µg/zone for (1) and 1-6 µg/zone for (2). LOD and LOQ were 131 and 430 ng/zone for (1) and 80 and 212 ng/zone for (2). Intermediate precision was below 2.9 %. Average recovery (by standard addition) for (1) and (2) was 100.2 % and 99.9 %, respectively.

Chinese J. of Food & Drug 14 (7), 284-286 (2012). Kexian capsules are a herbal TCM preparation for treating epilepsy and phlegm blockage syndrome. For quality control TLC, on silica gel 1) for Gambir plant, with toluene – chloroform – acetone – concentrated ammonia – methanol 20:25:15:4, detection under UV 366 nm; 2) for Borneol, with petroleum ether (60-90 °C) – ethyl acetate – chloroform 11:1:3, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight. Quantification of cholic acid by UV spectrophotometry.

J. Anhui Med. & Pharm. 17 (2), 202-203 (2013). Banlangen granule, processed of Radix Isatidis, is a traditional Chinese herbal medicine prescribed clinically to treat sore throat, cheek swelling, acute and chronic tonsillitis and mumps. For quality control a method for the determination of leucine, a main antiviral effective component, is presented. TLC on silica gel with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C and viewing in daylight. Quantification by densitometry at 509 nm. Validation of the method by investigation of the linearity range (0.10-0.90 µg/zone, r = 0.998, n=5), precision (%RSD = 2.8 %, n=6), stability (%RSD = 0.8 %, n=6, towards single lane within one hour), and reproducibility (%RSD = 2.0 %, n=6, within plate). The recovery (by standard addition) was 98.8 % (%RSD = 1.1 %, n=6).

species by validated HPTLC–densitometric method. J. Planar Chromatogr. 26, 248-253 (2013). HPTLC of ardisicrispin A in the roots, stems, leaves, fruits and flowers of Lysimachia nemorum L. (1), Lysimachia nummularia L. (2), Lysimachia vulgaris L. (3), Lysimachia punctata L. (4), Lysimachia thyrsiflora L. (5), Lysimachia ephemerum L. (6), Lysimachia ciliata L. (7), and Lysimachia clethroides Duby (8) on silica gel with chloroform - methanol - water 8:7:1 for (1), (3) and (7) and n-butanol - acetic acid - water 6:1:3 for (2), (4), (5), (6) and (8). Detection by spraying with 25 % sulfuric acid in methanol, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 545 nm. The hRf values for ardisicrispin A in both development systems were 86 and 36, respectively. Linearity was in the range of 2-13 µg/zone. LOD and LOQ were 620 and 950 ng/zone for the chloroform mobile phase and 1890-2880 ng/zone for the n-butanol mobile phase. Average recovery was in the range of 94.3-95.1 %. Intermediate/interday/intra-day precision was below 4 % (n=3).

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 19 (4), 70-71 (2013). Pothos chinensis (Raf.) Merr. and three other medicinal herbs are used in traditional Yao medicine, they have anti venom, anti tumor, and antioxidant effects and reduce blood sugar. For quality control, TLC of 1) Pothos chinensis (Raf.) Merr., on polyamide phase with acetone – water 1:2, detection by spraying with 2 % aluminiumchloride in ethanol and heating at 105°C until the zones are visible in daylight, and under UV 366 nm; 2) for Mappianthus iodoides Hand.-Mazz., on silica gel with petroleum ether (60-90°C) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight, and under UV 366 nm; 3) Zanthoxylum nitidum (Roxb.) DC., on silica gel with chloroform – methanol – ammonia 150:5:1, detection under UV 366 nm; 4) for Saussurea woodiana Hemsl., on silica gel with petroleum ether (60-90°C) – ethyl formate – formic acid 75:25:2, detection under UV 366 nm.

in Shanhaidanpian tablets) (Chinese). J. of China Pharm. 26 (9), 997-999 (2012). Shanhaidanpian tablets are a herbal TCM preparation for the treatment of poor blood flow, blood stasis and chest pain syndrome. For quality control, TLC on silica gel with toluene – ethyl acetate – formic acid 20:2:1, detection by exposure to vapors until the zones are visible in daylight. Quantitative determination of 2,3,5,4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC.

Chinese J. of Med. Guide 10 (20), 99-102 (2013). Fufang Xiaoji Zhike Milian Gao ointment is herbal TCM preparation for clearing the body heat caused by inflammation, resolving phlegm and relieving asthma. For quality control, TLC on silica gel 1) for Pericarpium Citri Reticulatae and the standard hesperidin, first over 3 cm with ethyl acetate – methanol – water 99:17:13 and thenover 8 cm with toluene – ethyl acetate – formic acid – water 95:45:5:3, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Schisandra Chinensis and the standard schizandrin A, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; 3) for Crataegus pinnatifida and the standard ursolic acid, with toluene – ethyl acetate – formic acid – water 39:8:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 80 °C, detection under UV 366 nm. Quantification of tenuifolin by HPLC.

J. Planar Chromatogr. 27, 181-185 (2014). HPTLC of plumbagin in the root of Plumbago zeylanica on silica gel with n-butanol - chloroform - petroleum 2:3:1. Quantitative determination by absorbance measurement at 268 nm. The hRF value for plumbagin was 58. Linearity was in the range of 225-675 ng/zone. The intermediate/interday/intra-day precision were below 2 % (n=3). The LOD and LOQ were 30 and 90 ng/zone, respectively.