Abstract No. 9933, IHCB (2009). HPTLC of colchicines in Gloriosa superba (collected from different parts of India) on silica gel with ethyl acetate - methanol 200:27. The hRf value of colchicine was 29. Quantitative determination by absorbance measurement at 350 nm. The method was linear in the range of 50-1000 ng/spot. The sample collected from Kerala was found to contain highest level of colchicines (0.24 %).

International Seminar on Herbal Drug Research, PN-064 (2009). HPTLC of gallic acid as marker compound in triphala fast dispersable tablets on silica gel with ethyl acetate - toluene - methanol - glacial acetic acid 75:20:3:2. Results from HPLC analysis were comparable. The method was suitable for routine quality control of dispersable tablets formulation.

J. AOAC Int. 93, 492-495 (2010). HPTLC of glycyrrhizin on silica gel with chloroform - methanol - water 130:72:15 in a twin-trough chamber saturated for 30 min. Detection under UV 254 nm and after spraying with anisaldehyde-sulfuric acid reagent. The hRf value of glycyrrhizin was 22. Quantitative determination by densitometry at 254 nm. The linearity was between 0.96-4.80 µg/spot, the correlation coefficient was r = 0.99904 and the standard deviation was 2.52 %. Average recovery was 99.6 %. LOQ and LOD was 246 and 81 ng/spot.

Abstract No. C-293, 61st IPC (2009). HPTLC of gallic acid in fruits of Phyllanthus emblica on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. The hRf value of gallic acid was 40. Quantitative absorbance measurement at 280 nm. The method was linear in the range of 40-240 ng/band. The method was reproducible and suitable for quality control.

International Journal of ChemTech Research 1(4), 1122-1128 (2009). TLC on silica gel with toluene - ethyl acetate 3:1. The hRf value of the furanocoumarin psoralen was 47. Densitometric evaluation at 299 nm. The method was linear in the range of 10-100 ng/band. The average recovery was 99.7 %.

Journal of Pharmacy Research 2(5), 975-977 (2009). Quantitative analysis of jatamansone in jatamansi oil obtained by steam distillation of rhizome extracts of Nardostachys jatamansi (Valerianaceae). HPTLC on silica gel with petroleum ether - acetone 3:1 in a saturated twin trough chamber. The hRf value of jatamansone was 43. Densitometric evaluation at 285 nm. The method was linear in the range of 250-1500 ng/band. The recovery was 98.8-102.2 %. The oil was found to contain 20.3 % of jatamansone.

Indian Drugs 47(6), 31-38 (2010). Pods of Prosopis were extracted with different solvents and the extracts were subjected to phytochemical evaluation using 2D paper chromatography and HPTLC. Analysis of phenolic acids by two-directional paper chromatography with toluene - acetic acid - water 6:7:3 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. For detection of phytoconstituents HPTLC on silica gel with different mobile phases and detection wavelengths: 1) beta-sitosterol with toluene - methanol 9:1, measured at 258 nm, 2) coumaric acid with toluene - ethanol 4:1, measured at 292 nm, 3) quercetin with toluene - methanol 4:1, measured at 271 nm, and 4) caffeine with toluene - acetone 7:3, measured at 272 nm.

Application to the detection of antimicrobial compounds from Cordia gilletii De Wild (Boraginaceae). J. Planar Chromatogr. 23, 245-249 (2010). TLC of methanol extracts of Cordia gilletii on silica gel with dichloromethane, dichloromethane - methanol 9:1 or 4:1, or petroleum ether - diethyl ether - acetic acid 90:10:1. Detection by spraying with 1 % vanillin in ethanol followed immediately by 10 % ethanolic sulfuric acid, and heating at 105 °C for 10 min. For bioautography, 1 mL of 0.5 McFarland microorganism suspension was diluted with 9 mL of the tested mixture of Mueller-Hilton broth and agar 9:1) at 37 °C. After distribution of the inoculated mixture over the plate and solidification of the medium at ambient temperature, the plate was incubated overnight at 37 °C. The bioautogram was subsequently sprayed with an aqueous solution of MTT 0.8 mg mL-1 and incubated at 37 °C for 4 h. Also application of the Bioluminex assay (a TLC detection method based on the natural bioluminescence of the non-pathogenic bacteria Vibrio fischerii). The bioluminescent bacterial coating was visualized by use of the Bioluminizer.