J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

Phytochemistry 23, 2505-2508 (1984). TLC of oleanolic acid glycosides on silica with the lower layers of chloroform - methanol - water 12:7:1 or chloroform - methanol - propanol - water 9:10:1:8. Detection with Komarousky reagent, Godin reagent and citrate blood reagent. Separation of oligosaccharide moieties split-off from the saponins by HPTLC on silica with isopropanol - pyridine - water 3:1:1, followed by hydrolysis on the HPTLC plate (by exposure to HCl, 30 min. at 100 °C) and subsequent chromatography in the second direction with chloroform - methanol - water 32:25:5.

Phytochemistry 25, 2501-2503 (1986). TLC of oleanolic acid glycosides on silica with chloroform - methanol - water 6:4:1. Detection with Godins reagent. After hydrolysis of the saponins, the aglycones are identified by TLC on silica with isopropylether -acetone 3:1 and the sugars by TLC on silica with ethyl acetate - methanol - formic acid water 13:3:4:3. Detection with naphthoresorcinol.

Phytochemistry 30, 3625- 3629 (1991). TLC of polyhydroxylated xanthones and their glycosides on silica with ethyl acetate - petrol ether 1:1 cont. 0.05% TFA and with chloroform - methanol 7:3; and HPTLC on RP-8 with methanol - water 9:1 and on RP-18 with methanol - water 3:2. Detection by spraying with 5% KOH in methanol. Also column chromatography and medium pressure liquid chromatography.

Planta med. 70, 143-146 (2004). Analytical and preparative TLC of hispidin (6-(3,4-dihydroxystyryl)-4-hydroxy-2-pyrone) on silica gel with benzene - ethyl acetate - acetic acid 10:1:1. Detection by spraying with iron(III) chloride reagent.

Planta med. 68, 770-775 (2002). Preparative and analytical TLC of chelirubine, sanguinarine, macarpine, and chelerythrine on silica gel and aluminium oxide with chloroform - methanol 49:1. Detection under UV light.

Planta med. 70, 59-64 (2004). Analytical and preparative TLC of withasteroids (physachenolide E and D) on silica gel with n-hexane - acetone 6:5 by 8-fold development and chloroform - acetone 7:3 by 4-fold development. Detection under UV light and by spraying with cerium sulfate - sulfuric acid reagent.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (8), Appendix 22-24 (2004). Study of the quality standard of Naoxinkang pills by TLC in order to optimize the manufacture technology and clinical application. TLC of Naoxinkang pill extracts on silica gel with 1) the lower phase of chloroform - methanol - water 13:7:2; 2) chloroform - methanol - water 28:8:1; 3) cyclohexane - ethyl acetate 9:1. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC; 2) under UV 365 nm. Identification by fingerprint technique. Discussion of employing the procedures in the optimization of the manufacture technology and its clinical application for the medicine.