J. AOAC Int. 94, 1422-1426 (2011). HPTLC of eleutherosides B, E, and E1 in the fruits and leaves of E. senticosus and five other species of Eleutherococcus species after extraction with SPE, on silica gel in the horizontal chamber with saturation for 20 min, with a two-step development with 1) chloroform - methanol - water 70:30:4 and 2) chloroform - methanol - toluene - ammonium hydroxide 9:6:3:2. Detection by immersion in Liebermann-Burchard reagent for 1 s and heating at 110 °C for 10 min. The hRf values for the eleutherosides E, B, and E1 were 43, 53, and 66, respectively.

Anal. Chim. Acta 633 (2) 188-196 (2009). HPTLC of lycorine and galanthamine from Narcissus jonquilla ‘Pipit’ on silica gel with chloroform - methanol - 25 % ammonia 18:1:1. Quantitative evaluation by absorbance measurement at 207 nm. The correlation coefficients were r=0.9882 and 0.9908, respectively, for the mean values of galanthamine and lycorine. Investigation of different extraction solvents showed that extraction with methanol and 1 % tartaric acid in methanol at default conditions (120 °C, p = 60 bar, time: 10 min, one static cycle) provide the highest yields of total alkaloids, whereas for toluene the lowest amounts were measured. Lycorine to galanthamine mean ratios were dependant on the type of solvent used, and in toluene galanthamine and related alkaloids were preferably extracted.

Asian Journal of Chemistry 23(5), 1917-1921 (2011). TLC of concentrated methanolic extracts of a polyherbal ayurvedic syrup formulation (containing vasaka as main ingredient) on silica gel with methanol - toluene - dioxane - 25 % ammonia 2:2:5:1. The hRf value of vasicine was 74. Quantitative evaluation by absorbance measurement at 254 nm using vasicine as marker for standardization of the formulation. The method was found to be linear in the range of 4-12 ng/band. Isolation of vasicine from Adhatoda varica is also described.

J. Planar Chromatogr. 24, 312-315 (2011) HPTLC of methanolic extracts of L. tibetica on silica gel (prewashed with methanol) with hexane - ethyl acetate - formic acid 12:7:1 for three furofuranolignans (sylvatesmin, (+)-piperrtol, horsfieldin), beta-sitosterol, and oleanolic acid, and with chloroform - methanol - water - formic acid 70:25:4:2 for four furofuranolignans (phillyrin, tibeticoside A, lantibeside C, and lantibeside), verbascoside and isoverbascoside in a twin-trough chamber with saturation for 20 min. Detection by immersion in ethanolic sulfuric acid for 2 s followed by heating for 5 min at 100 °C. The hRf values were 17, 25, 29, 51, 60, 70, 66, 49, 46, 19, and 21 for sylvatesmin, (+)-piperrtol, horsfieldin, oleanolic acid, beta-sitosterol, phyllirin, tibeticoside A, lantibeside C, lantiboside, verbascoside and isoverbascoside, respectively.

J. Planar Chromatogr. 25, 403-408 (2012). HPTLC of rosmarinic acid in Salvia species on silica gel with ethyl acetate - toluene - formic acid 6:3:1. Quantitative determination by absorbance measurement at 328 nm. The hRf value of rosmarinic acid was 42. Linearity was in the range of 200-1000 ng. The coefficient of variation (%) of intra-day and inter-day precision were 1.4 and 4.5, respectively (n=9). Recovery (by standard addition) was between 82.3 and 85.9 %.

J. Planar Chromatogr. 25, 575-580 (2012). HPTLC of echioidinin-5-O-beta-D-glucopyranoside in Andrographis echioides on silica gel with chloroform - methanol 31:9. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 200-1400 ng/zone. Limits of detection and quantification were found to be 54 and 68 ng/zone, respectively. Recovery (by standard addition) was 96.7 %.

J. of Yangtze Univ. (Nat. Sci., Med.) 9 (1), 64-65 (2012) . Angelica sinensis is a herbal TCM drug widely used in the preparations for gynaecological diseases, effective in promoting blood circulation, ease of pain and against inflammations. TLC of the extracts of the preparation on silica gel with n-hexane – ethyl acetate 9:1. Detection under UV 366 nm.

(Papaveraceae) tissues by TLC–in situ fluorography. J. Planar Chromatogr. 25, 358-360 (2012). TLC of berberine and sanguinarine on silica gel with n-butanol - water - ammonia 8:1:1. Detection by densitometry in fluorescence mode at 325 nm. The hRf of berberine and sanguinarine were 29 and 95, respectively.