collected from different phyto-geographical zones of India. J. Liq. Chromatogr. Relat. Technol. 36, 1810-1821 (2013). HPTLC of protodioscin (1) and prototribestin (2) in fruits of Tribulus terrestris on silica gel with n-butanol - glacial acetic acid - water 40:3:10. Quantitative determination by absorbance measurement at 366 nm. The hRf values of (1) and (2) were 41 and 39, respectively. Linearity was in the range of 100-1000 ng/zone for both (1) and (2). LOD and LOQ were 40 and 100 ng/zone for (1) and 35 and 100 ng/zone for (2). Intermediate precision was below 1.0 %. Recovery ranged 98.0-103.0 % for (1) and 99.1-101.9 % for (2).

J. Gansu Coll. of Trad. Chinese Med. 29 (6), 19-22 (2012). Yanyan tablets are a herbal TCM for treating chronic pharyngitis. For quality control, TLC on silica gel 1) for Radix Stemonae, with petroleum ether (60-90 °C) – methanol – ethyl acetate – ethylenediamine 20:20:40:1, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and viewing in daylight; 2) for Radix Ophiopogonis and flowers of common coltsfoot, with petroleum ether (60-90 °C) – acetone 6:1, detection under UV 254 nm. Quantification of rutin by HPLC.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (1), 116-117 (2011). Notopterygium root is the dried root of Notopterygium itwisum Ting ex H. T. Chang and Notopterygium forbesii Boiss and is used to relieve rheumatic pains. Development of a method for qualitative and quantitative determination of nodakenin, one of the key active components in the crude drugs for quality control. TLC of the extracts of the drugs on silica gel with chloroform – methanol 4:1, detection at UV 366 nm. Quantification of nodakenin was done by HPLC.

J. of China Pharm. 22 (9), 14-16 (2013). Sanyu Xugu Tie plaster is a herbal TCM preparation for the treatment of traumatic injury, contusion, stasis of blood and innominate toxic swelling. For quality control, TLC on silica gel 1) for Radix Cynanchi paniculati and the standard 2'-hydroxy-4'-methoxyacetopheone, with cyclohexane – ethyl acetate 3:1, detection by spraying with 5 % ferric chloride in ethanol and heating until the zones are visible in daylight; 2) for Oleum eucalypti and the standard cineole, with cyclohexane – ethyl acetate 19:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 3) for Herba sarcandrae and the standard isofraxidin, with toluene – ethyl acetate – formic acid 9:4:1, detection under UV 366 nm; 4) for Kusnezoff monkshood root and the standard acetylbenzoylaconine, with cyclohexane – ethyl acetate diethylamine 12:8:1, detection by spraying with 5 % potassium iodobismuthate in water – hydrochloric acid 200:1 and viewing in daylight.

Chinese J. Mod. Drug Appl. 7 (17), 228-229 (2013). Zhuangyao Zhitong Heji compound oral liquid is a herbal TCM preparation to treat deficiency of the kidney and liver, chills and pain of the waist and knee, wind-cold-dampness arthralgia and low back injury. For quality control, TLC on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm. Identification of Angelica sinensis, Ligusticum chuanxiong and Saposhnikovia divaricata roots by fingerprint comparison with reference drugs.

Chinese J. of Northern Pharmacy 10 (3), 5-6 (2013). Fufang Huoxue Tongluojiu liquor is a herbal TCM preparation for easing traumatic injury, soft tissue injury, arthralgia, and muscle soreness. For quality control, TLC on silica gel 1) for Caesalpiniaceae, with chloroform – acetone – formic acid 8:4:1, detection by heating at 105 °C until the zones are visible in daylight; 2) for Rhizoma Corydalis and the standard tetrahydropalmatine, with toluene – acetone 9:2, detection by exposure to iodine vapors for 3 min and viewing under UV 366 nm; 3) for Flos Carthami, with chloroform – ethyl acetate – glacial acetic acid 5:4:1, detection under UV 254 nm; 4) for semi quantification of aconitine, with chloroform – ethyl acetate 1:1, detection by saturation with ammonia vapors and spraying with 5 % potassium iodobismuthate in hydrochloric acid – water 1:200 and viewing in daylight. Semi quantitative analysis by comparison with aconitine.

Chinese J. of Ethnomed. & Ethnopharm. 3, 41-43 (2012). Yufukang Jiaonang capsules are a herbal TCM preparation for the treatment of numbness of the tongue, language disadvantage, choke and cough when eating, aphasia, etc. For quality control, identification of the main component crude drug, the root of Salvia miltiorrhiza and the standard tanshinone llA by TLC on silica gel with toluene – ethyl acetate 19:1, detection in daylight.

J. Planar Chromatogr. 27, 42-46 (2014). HPTLC of beta-sitosterol in the root cultures of Clitoria ternatea on silica gel with n-hexane - acetone 4:1. Detection by dipping into 5 % methanol - sulfuric acid reagent, followed by heating at 105 ºC for 3 min. Quantitative determination by absorbance measurement at 414 nm. The hRF value for beta-sitosterol was 31. Linearity was in the range of 100-500 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 40 and 100 ng/zone, respectively. Recovery was between 97.2 and 98.5 %.